Controlled fragmentation of DNA by DNase I

doi:10.1016/0003-2697(72)90089-9

Analytical Biochemistry

Volume 48, Issue 2, August 1972, Pages 369-377

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Abstract

Large amounts of purified DNA have been prepared by a method based on that of Britten et al. (3). This DNA is of relatively uniform molecular weight and can be sheared reproducibly by DNase I to yield small molecular weight fragments. Large-scale preparations using alkaline sucrose gradients in a titanium BXIV zonal rotor yield milligram amounts of DNA fragments of defined size range.

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Controlled fragmentation of DNA by DNase I

Abstract

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