Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies

https://doi.org/10.1016/0003-2697(66)90246-6Get rights and content

Abstract

A method is deseribed for quantitative analysis of proteins with a charge differing from that of the bulk of the immunoglobulins. The method utilizes the difference between the rate of electrophoretic migration of proteins and of their antibody complexes in agarose gel.

The method has an error of a few per cent; it is rapid, suitable for serial analysis, and requires 2 to 0.5 μg protein antigen. The amount of antiserum needed is slightly less than with the radial immunodiffusion techniques.

References (7)

  • A. Bussard

    Biochim. Biophys. Acta

    (1959)
  • N. Ressler

    Clin. Chim. Acta

    (1960)
  • C.-B. Laurell

    Anal. Biochem

    (1965)
There are more references available in the full text version of this article.

Cited by (0)

View full text