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Cobalt-induced retrotransposon polymorphism and humic acid protection on maize genome

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Abstract

Retrotransposon activity and genomic template stability (GTS) are one of the most significant rearranging mechanisms in environmental stress. Therefore, in this study, it is aimed to elucidate effecting of Cobalt (Co) on the instability of genomes and Long Terminal Repeat retrotransposon polymorphism in Zea mays and whether humic acid (HA) has any role on these parameters. For this purpose, Retrotransposon-microsatellite amplified polymorphism (REMAP) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers were applied to evaluate retrotransposon polymorphism and the GTS levels. It was found that IRAP and REMAP primers generate unique polymorphic band structures on maize plants treated with various doses of Co. Retrotransposon polymorphism increased and GTS decreased while increasing Co concentration. On the other hand, there was a reduction in negative effects of Co on retrotransposon GTS and polymorphism after treatment with HA. The results indicate that HA may be used effectively for the protection of maize seedlings from the destructive effects of Co toxicity.

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The datasets used and analyzed during the current study are provided in complete in the ‘Results’ section of this paper.

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Acknowledgements

We thank all of the participants in the study.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Esma Yigider, Mahmut Sinan Taspinar & Murat Aydin

  2. Department of Biology, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey

    Guleray Agar

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  1. Esma Yigider
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  2. Mahmut Sinan Taspinar
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  4. Guleray Agar
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Contributions

MST and EY designed the study. MST realized that the submitting and the spelling of the article. Applications, DNA isolation and IRAP and REMAP PCR reactions were evaluated and performed by MA and EY. GA and EY analyzed IRAP and REMAP assay. The authors have brought together their data. All authors approved the final manuscript.

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Correspondence to Mahmut Sinan Taspinar.

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Yigider, E., Taspinar, M.S., Aydin, M. et al. Cobalt-induced retrotransposon polymorphism and humic acid protection on maize genome. BIOLOGIA FUTURA 71, 123–130 (2020). https://doi.org/10.1007/s42977-020-00001-z

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