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Recent Advances in Visual Electrochemiluminescence Analysis

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Abstract

Due to the advantages of low background interference and high sensitivity, electrochemiluminescence (ECL)-based sensor has developed rapidly in recent years. The ECL sensors have shown the potential in the ultrasensitive and real-time analysis. Especially, the visual ECL analysis, including visual detection, cell imaging and single particle analysis, has offered many unique sensing platforms for analysis research and point-of-care testing. The high throughput ECL image analysis can not only provide ECL intensity but also reveal more information about the chemical reaction activity of particle and the physiological processes of cell operation. Therefore, we review the novel ECL luminophore, sensing systems, and successful applications in the visual ECL detection and imaging in this paper. First, the different ECL luminophore is summarized. Second, we discuss the ECL sensing mechanisms, focusing on the advantages and limitations of different sensing methods. Then, we highlight the recent advances in representative examples of visual ECL analysis, including aptasensing, multiplex immunoassays, cell imaging and single-particle analysis. At last, the outlook and prospects for the future visual ECL analysis are discussed based on the current development of ECL research.

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Fig. 1

Reprinted with permission from Ref. [45], American Chemical Society

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Reprinted with permission from Ref.[52], American Chemical Society

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Reprinted with permission from Ref. [27], American Chemical Society

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Reprinted with permission from Ref. [58], Elsevier

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Reprinted with permission from Ref. [59], Elsevier

Fig. 6

Reprinted with permission from Ref.[73], American Chemical Society

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Reprinted with permission from Ref. [43], American Chemical Society

Fig. 8

Reprinted with permission from Ref. [51], Elsevier

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Reprinted with permission from Ref. [61], Elsevier

Fig. 10

Reprinted with permission from Ref. [83], John Wiley and Sons

Fig. 11

Reprinted with permission from Ref. [84], American Chemical Society

Fig. 12

Reprinted with permission from Ref. [62], American Chemical Society

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Acknowledgements

The authors gratefully acknowledge financial support from Youth Science Fund of Jilin Province (20140520081JH) and “Thirteenth Five Year” Project of the Science and Technology Research in the Education Department of Jilin Province, China.

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  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China

    Qian Zhang, Xin Zhang & Qiang Ma

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  1. Qian Zhang
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  2. Xin Zhang
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  3. Qiang Ma
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Correspondence to Qiang Ma.

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Zhang, Q., Zhang, X. & Ma, Q. Recent Advances in Visual Electrochemiluminescence Analysis. J. Anal. Test. 4, 92–106 (2020). https://doi.org/10.1007/s41664-020-00129-w

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