Skip to main content
SpringerLink
Log in

Bentazone herbicide induces genotoxic effect and physiological disorders in non-targeted Allium cepa L.

  • Short Communication
  • Published:
Indian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Bentazone {3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide} categorized under thiadiazine group is a selective herbicide that is widely used for weed control in cereals, onions, garlic and other related crops. Field and laboratory experiments were conducted for 5 weeks to assess some of the responses of Allium cepa (a non-target plant) to herbicide treatment. Growth, physiological, cytotoxic and genotoxic parameters were determined. The result showed that bentazone significantly decreased plant growth and protein content; while level of malondialdehyde increased by 56.25 %. Chromosomal aberration and DNA damage were also associated with bentazone treatment on A. cepa. It was concluded that bentazone herbicide could cause genotoxicity and physiological disorders in non-targeted plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Ashton, F. M., & Crafts, A. S. (1973). Mode of action of herbicides. New York: Wiley.

    Google Scholar 

  • Badr, A., Ghareeb, A., & El-Din, H. M. (1992). Cytotoxicity of some pesticides in mitotic cells of V. faba roots. Egyptian Journal of Applied Sciences, 7, 457–468.

    Google Scholar 

  • Bradshaw, L., Barret, M., & Poneleit, C. (1992). Physiological basis for differential bentazone susceptibility among corn (Zea mays L.) inbreds. Weed Science, 40, 522–527.

    CAS  Google Scholar 

  • Burton, J. D., & Maness, E. P. (1992). Constitutive and inducible bentazone hydroxylation in Shatter cane (Sorghum bicolor) and Johnson grass (Sorghum halapense). Pesticide Biochemistry and Physiology, 44, 40–49.

    Article  CAS  Google Scholar 

  • Devine, M. D., & Preston, C. (2000). The molecular basis of herbicide resistance. In A. H. Cobb & R. C. Kirkwood (Eds.), Herbicides and their mechanisms of action (pp. 72–104). Sheffield: CRC Press.

    Google Scholar 

  • Eastin, E. F. (1978). Total nitrogen determination for plant material containing nitrate. Analytical Biochemistry, 85, 91–594.

    Article  Google Scholar 

  • Evseeva, T. I., Geras’kin, S. A., Shuktomova, I. I., & Taskaev, A. I. (2005). Genotoxicity and cytotoxicity assay of water sampled from the underground nuclear explosion site in the north of the Perm region (Russia). Journal of Environmental Radioactivity, 80, 59–74.

    Article  CAS  PubMed  Google Scholar 

  • Fleming, A. A., Banks, P. A., & Legg, J. G. (1988). Differential response of maize inbreds to bentazone and other herbicides. Canadian Journal of Plant Science, 68, 501–507.

    Article  CAS  Google Scholar 

  • Fusconi, A., Repetto, O., Bona, E., Massa, N., Gallo, C., Dumas-Gaudot, E., & Berta, G. (2006). Effect of cadmium on meristem activity and nucleus ploidy in roots of Pisum sativum L. cv. Frisson seedlings. Environmental and Experimental Botany, 58, 253–260.

    Article  CAS  Google Scholar 

  • Gronwald, J. W., & Connelly, J. A. (1991). Effect of monooxygenase inhibitors on bentazone uptake and metabolism in maize cell suspension cultures. Pesticide Biochemistry and Physiology, 40, 284–294.

    Article  CAS  Google Scholar 

  • Hess, F. D. (2000). Light-dependent herbicides: An overview. Weed Science, 48, 160–170.

    Article  CAS  Google Scholar 

  • Liman, R., Akyil, D., Eren, Y., & Konuk, M. (2010). Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test. Chemosphere, 80, 1056–1061.

    Article  CAS  PubMed  Google Scholar 

  • Michelle-Frainer, K., Antonio, C. F., Thais, S., & Solange, B. T. (2006). Effects of Pterocaulon polystachyum DC. (Asteraceae) on Onion (Allium cepa) root-tip cells. Genetics and Molecular Biology, 29, 539–542.

    Article  Google Scholar 

  • Nemat Alla, M. M., Badawi, A. M., Hassan, N. M., El-Bastawisy, Z. M., & Badran, E. G. (2008). Effect of metribuzin, butachlor and chlorimuron-ethyl on amino acid and protein formatin in wheat and maize seedlings. Pesticide Biochemistry and Physiology, 90, 8–18.

    Article  CAS  Google Scholar 

  • Osama, A., & Floyd, M. A. (1984). Bentazone influence on selected metabolic processes of isolated bean leaf cells. Journal of Plant Growth Regulation, 3, 121–126.

    Article  Google Scholar 

  • Ozakca, D. U., & Silah, H. (2013). Genotoxicity effects of Flusilazole on the somatic cells of Allium cepa. Pesticide Biochemistry and Physiology, 107, 38–43.

    Article  CAS  PubMed  Google Scholar 

  • Pateiro-Moure, M., Arias-Estévez, M., López-Periago, E., Martínez-Carballo, E., & Simal-Gándara, J. (2008). Occurrence and downslope mobilization of quaternary herbicide residues in vineyard-devoted soils. Bulletin of Environmental Contamination and Toxicology, 80, 407–411.

    Article  CAS  PubMed  Google Scholar 

  • Qian, H., Hu, H., Mao, Y., Ma, J., Zhang, A., Liu, W., & Fu, Z. (2009). Enantioselective phytotoxicity of the herbicide imazethapyr in rice. Chemosphere, 76, 885–892.

    Article  CAS  PubMed  Google Scholar 

  • Ramalho, J. C., Pons, T. L., Groeneveld, H. W., Azinheira, H. G., & Nunes, M. A. (2000). Photosynthetic acclimation to high light conditions in mature leaves of Coffea arabica L.: Role of xanthophylls quenching mechanisms and nitrogen nutrition. Australian Journal of Plant Physiology, 27, 43–51.

    CAS  Google Scholar 

  • Rutherford, A. W., & Krieger-Liszkay, A. (2001). Herbicide-induced oxidative stress in photosystem II. Trends in Biochemical Science, 26, 648–653.

    Article  CAS  Google Scholar 

  • Saxena, P. N., Gupta, S. K., & Murthy, R. C. (2010). Carbofuran induced cytogenetic effects in root meristem cells of Allium cepa and Allium sativum: A spectroscopic approach for chromosome damage. Pesticide Biochemistry and Physiology, 96, 93–100.

    Article  CAS  Google Scholar 

  • Sharma, K. K., Lavanya, M., Sharma, V., Lavanya, M., & Anjaiah, V. (2000). A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Molecular Biology Reporter, 18, 393a–393h.

    Article  Google Scholar 

  • Sharma, S., & Vig, A. P. (2012). Genotoxicity of Atrazine, Avenoxan, Diuron and Quizalofop-P-ethyl herbicides using the Allium cepa root chromosomal aberration assay. Terrestrial and Aquatic Environmental Toxicology, 6, 90–95.

    Google Scholar 

  • Shigenaga, M. K., & Ames, B. N. (1991). Assays for 8-Hydroxy-2′-deoxyguanosine: A biomarker of in vivo oxidative DNA damage. Free Radical Biology and Medicine, 10, 211–216.

    Article  CAS  PubMed  Google Scholar 

  • Sterling, T. M., & Balke, N. E. (1989). Differential bentazone metabolism and retention of bentazone metabolites by plant cell cultures. Pesticides Biochemistry and Physiology, 34, 39–48.

    Article  CAS  Google Scholar 

  • Ukaegbu, M. C., & Odeigah, P. G. C. (2009). Genotoxic effects of sewage effluent on Allium cepa. Rep Opin, 1, 36–41.

    Google Scholar 

  • Wang, H., & Jin, J. Y. (2005). Photosynthetic rate, chlorophyll fluorescence parameters, and lipid peroxidation of maize leaves as affected by zinc deficiency. Photosynthetica, 43, 591–596.

    Article  CAS  Google Scholar 

  • Wang, Y., Lu, X., Sun, M., Song, F., Li, L., Gao, T., & Yan, J. (2008). Physiological research on the difference of bentazone tolerance in wild type rice and sensitive lethal mutants. Acta Agronomica Sinica, 34, 1615–1622.

    Article  CAS  Google Scholar 

  • Wu, C., & Wang, C. (2003). Physiological study on bentazone tolerance in inbred corn (Zea mays). Weed Technology, 17, 565–570.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Lagos, Akoka, Lagos, Nigeria

    V. J. Odjegba & R. A. Adeniran

Authors
  1. V. J. Odjegba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Adeniran
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. J. Odjegba.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Odjegba, V.J., Adeniran, R.A. Bentazone herbicide induces genotoxic effect and physiological disorders in non-targeted Allium cepa L.. Ind J Plant Physiol. 20, 375–379 (2015). https://doi.org/10.1007/s40502-015-0168-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40502-015-0168-1

Keywords

Search

Navigation