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Enhanced topical delivery of fish scale collagen employing negatively surface-modified nanoliposome

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Abstract

In this study, we investigated the effect of fish scale collage (FSC) on type I procollagen expression in cultured human dermal fibroblasts using real-time polymerase chain reaction and ELISA, under normal and UV exposure condition. Also, FSC encapsulated negatively surface modified nanoliposome (NLsurf mod) was fabricated, and the physicochemical characteristics of the liposomes and skin permeation properties were evaluated. FSC increased type I procollagen mRNA and protein expression in human dermal fibroblasts. The mean particle size, zeta potential value and encapsulation efficiency of the NLsurf mod were about 175 nm, −44 mV and 11%, respectively. The skin localization effect was enhanced by NLsurf mod. Conclusively, FSC is able to elevate the type I procollagen expression in human dermal fibroblasts and FSC-loaded NLsurf mod would be a good candidate for the topical delivery of FSC.

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Acknowledgements

The present research has been supported by the Korea Research Foundation (KRF) grant funded by the Korea government (MEST) (No. 2009–0067835) and Genom & Medicine. All authors (J. Seo, M.J. Kim, S.O. Jeon, D.H. Oh, K.H Yoon, Y.W. Choi, S. Bashyal and S. Lee) declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Dalseo-Gu, Daegu, 42601, Republic of Korea

    Joeun Seo, Sang-Ok Jeon, Dong-Ho Oh, Santosh Bashyal & Sangkil Lee

  2. Department of Smart Foods and Drugs, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 50834, Republic of Korea

    Min-Ju Kim, Sang-Ok Jeon & Dong-Ho Oh

  3. Genom & Medicine, Gwangpyung building 901, Chunho-daero, Dongdaemun-gu, Seoul, 02633, Republic of Korea

    Kyu-Hyung Yoon

  4. College of Pharmacy Chung-Ang University, Heuksuk-ro 47, Dongjak-gu, Seoul, 06911, Republic of Korea

    Young Wook Choi

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  1. Joeun Seo
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  2. Min-Ju Kim
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  3. Sang-Ok Jeon
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  4. Dong-Ho Oh
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  5. Kyu-Hyung Yoon
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  6. Young Wook Choi
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  7. Santosh Bashyal
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  8. Sangkil Lee
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Correspondence to Sangkil Lee.

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Seo, J., Kim, MJ., Jeon, SO. et al. Enhanced topical delivery of fish scale collagen employing negatively surface-modified nanoliposome. J. Pharm. Investig. 48, 243–250 (2018). https://doi.org/10.1007/s40005-017-0303-2

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  • DOI: https://doi.org/10.1007/s40005-017-0303-2

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