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Formulation and ex-vivo evaluation of metronidazole microemulsion loaded hydrogel for prevention of periodontitis

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Abstract

Periodontal disease comprises a group of inflammatory conditions of periodontal tissues with common etiologic agent (bacteria) in the form of dental plaque. The objective of this research was to prepare a water-in-oil type microemulsion comprising metronidazole and equate its potency towards the effective treatment of periodontitis. A pseudo ternary phase diagram for microemulsion investigated using quaternary system containing water/captex 500/ tween 80/acconon CC6. The microemulsion was preferred from the microemulsion area on the phase diagram. Stable water-in-oil type metronidazole microemulsion was prepared successfully using the quaternary system of water/captex 500/ tween 80/acconon CC6 at 4:1 ratio having droplet size in the range of 81 ± 12.91 to 196 ± 10.73 nm, conductivity 50.6 ± 0.8 to 330.7 ± 1.1 μs/cm. In-vitro drug release, in vitro and ex vivo antimicrobial activities by agar well diffusion were investigated. Metronidazole Microemulsion loaded hydrogel formulations (F9 and F10) were found to be optimized for maximum release and antimicrobial activity in terms of zone of inhibition. The in vitro release evidenced that metronidazole microemulsion loaded hydrogel release rate was maximum as compared to other plain metronidazole gels. Stability study proved that microemulsion persisted stable for at least 6 months; with no changes in clarity, characteristic properties, and no sign of crystallization of metronidazole. In ex vivo evaluation, microemulsion based hydrogels were effective against the microbial flora of the human oral cavity suffering from periodontitis. The system was found to be appropriate for application and more effective in reducing the clinical symptoms of periodontitis.

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References

  • Abd-Allah FI, Dawaba HM, Ahmed MS (2010a) Development of a microemulsion-based formulation to improve the availability of poorly water-soluble drug. Drug Discov Ther 4:257–266

    CAS  PubMed  Google Scholar 

  • Abd-Allah FI, Dawaba HM, Ahmed AMS (2010b) Development of a microemulsion based formulation to improve the availability of poorly water-soluble drug. Drug Discov Therapeutics 4:257–266

    CAS  Google Scholar 

  • Ahmed TA, Ibrahim HM, Ibrahim F, Samy AM, Fetoh E, Nutan MT (2011) In vitro release, rheological, and stability studies of mefenamic acid coprecipitates in topical formulations. Pharm Dev Technol 16:497–510

    Article  CAS  PubMed  Google Scholar 

  • Albandar JM, Brunelle JA, Kingman A (1999) Destructive periodontal disease in adults 30 years of age and older in the United States, 1988–1994. J Periodontol 70:13–29

    Article  CAS  PubMed  Google Scholar 

  • Chen H, Chang X, Du D, Li J, Xu H, Yang X (2006) Microemulsion-based hydrogel formulation of ibuprofen for topical delivery. Int J Pharm 315:52–58

    Article  CAS  PubMed  Google Scholar 

  • Dhanikula AB, Panchagnula R (2004) Development and characterization of biodegradable chitosan films for local delivery of paclitaxel. AAPS PharmSciTech 6:88–99

    Article  Google Scholar 

  • Edwards PC, Kanjirath P (2010) Recognition and management of common acute conditions of the oral cavity resulting from tooth decay, periodontal disease, and trauma: an update for the family physician. J Am Board Fam Med 23:285–294

    Article  PubMed  Google Scholar 

  • Ercan E, Dulgergil T, Yavuz I (2006) The effects of antibacterial solutions on microorganisms isolated from infected root canals in vivo. Biotechnol Biotechnol Eq 20:149–156

    Article  CAS  Google Scholar 

  • Fontell K (1992) Some aspects on the cubic phases in surfactant and surfactant like lipid systems. Adv Colloid Interface Sci 41:127–147

    Article  CAS  Google Scholar 

  • Gordon JM, Walker CB (1993) Current status of systemic antibiotic usage in destructive periodontal disease. J Periodontol 64:760–771

    Article  CAS  PubMed  Google Scholar 

  • Hauss DJ (2007) Oral lipid-based formulations. Adv Drug Deliv Rev 59:667–676

    Article  CAS  PubMed  Google Scholar 

  • Jones DS, Woolfson AD, Brown AF, Coulter WA, Mcclelland C, Irwin CR (2000) Design, characterization and preliminary clinical evaluation of a novel mucoadhesive topical formulation containing tetracycline for the treatment of periodontal disease. J Contr Rel 67:357–368

    Article  CAS  Google Scholar 

  • Kanga BK, Leea JS, Chona SK, Jeongc SY, Yukb SH, Khanga G (2004) Development of self-microemulsifying drug delivery systems (SMEDDS) for oral bioavailability enhancement of simvastatin in beagle dogs. Int J Pharm 274:65–73

    Article  Google Scholar 

  • Khanna R, Agrawal SP, Ahuja A (1997) Preparation and evaluation of buccal films of clotrimazole for oral candida infections. Indian J Pharm Sci 59:299–305

    CAS  Google Scholar 

  • Lo I, Madsen F, Florence AT, Treguier JP, Seiller M, Puisieux F (1977) The influence of surfactant HLB and the nature of the oil phase diagrams of non-ionic surfactant oil water systems. J Colloid Interface Sci 59:319–325

    Article  CAS  Google Scholar 

  • Ming-Yan G, Zheng-Wu W, Hui-Xin Y, Gan-Zuo L (2006) Microemulsion of Tween 80/n Butyl alcohol/H2O System and Its Entrapment Efficiency of ATRA. J Dispers Sci Technol 27:949–954

    Article  Google Scholar 

  • Oliver RC, Brown LJ, Loe H (1998) Periodontal diseases in the United States population. J Periodontol 69:269–278

    Article  CAS  PubMed  Google Scholar 

  • Salarian M, Solati-Hashjin M, Shafiei SS (2009) Template-directed hydrothermal synthesis of dandelion-like hydroxyapatite in the presence of cetyltrimethyl ammonium bromide and polyethylene glycol. Ceram Int 35:2563–2569

    Article  CAS  Google Scholar 

  • Schick MJ (1977) Nonionic surfactants. Marcel Dekker, New York, pp 245–247

    Google Scholar 

  • Shukla D, Chakraborty S, Singh S, Mishra B (2011) Lipid-based oral multiparticulate formulations—advantages, technological advances and industrial applications. Expert Opin Drug Deliv 8:207–224

    Article  CAS  PubMed  Google Scholar 

  • Slots J, Ting M (2002) Systemic antibiotics in the treatment of periodontal disease. Periodontology 28:106–176

    Article  Google Scholar 

  • The United States Pharmacopeia (USP 24) (2000) The National Formulary (NF 19). By authority of the United States pharmacopeial convention. Rockville: vol II, USP convention, pp 1941–1944

  • Tirnaksiz F, Kayis A, Celebi N, Adisen E, Erel A (2012) Preparation and evaluation of topical microemulsion system containing metronidazole for remission in rosacea. Chem Pharm Bull 60:583–592

    CAS  PubMed  Google Scholar 

  • Zhu W, Guo C, Yu A, Gao Y, Cao F, Zhai G (2009) Microemulsion-based hydrogel formulation of penciclovir for topical delivery. Int J Pharm 378:152–158

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to Macleods Pharmaceuticals Ltd., Mumbai, India for providing metronidazole as a gift sample and Abitec Corporation, Janesville, USA for Captex 500, Acconon CC6. Authors are also thankful to Head Department of Pharmaceutical Sciences; RTM Nagpur University for proving research facilities.

Conflict of interest

All authors (S Pandey, U Das and A Patil) declare that they have no conflict of interest. No human or animal studies were performed in this study

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur, 440 033, Maharashtra, India

    Suneel Pandey, Ujjalkumar Das & Arun Patil

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  1. Suneel Pandey
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  2. Ujjalkumar Das
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  3. Arun Patil
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Correspondence to Suneel Pandey.

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Pandey, S., Das, U. & Patil, A. Formulation and ex-vivo evaluation of metronidazole microemulsion loaded hydrogel for prevention of periodontitis. Journal of Pharmaceutical Investigation 44, 225–236 (2014). https://doi.org/10.1007/s40005-014-0119-2

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  • DOI: https://doi.org/10.1007/s40005-014-0119-2

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