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Isolation and characterization of heavy-metal-resistant bacteria and their applications in environmental bioremediation

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Abstract

Heavy metal pollution is one of the emerging problems of environmental contaminations due to the rapid  industrialization of geologic and anthropogenic activities. Among further metals, chromium originates to be noxious due to its connotation with other metals. Therefore, the present investigation aims to isolate, identify and characterize the bacteria from soil samples and to evaluate their heavy metal (chromium) degrading efficiency under batch mode condition. Among the 300 bacterial isolates, two strains such as Pseudomonas fluorescens (YPS3 GenBank number—MH580200) and Bacillus safensis (YKS2 GenBank number—H539636) have found to possess effective chromium degrading capacity up to 84% and 72%, respectively, which was identified by morphological, biochemical and molecular characterization. The 16S rRNA phylogenetic association was resolute by means of bioinformatics and recognized as bacterial species YKS2 and YPS3 with 100% sequence resemblance. In conclusion, the findings show effective chromium degradation level by the bacterial strains in the contaminated environment soil. These biological mediated metal degradation methods will be an effective green alternative for polluted environment.

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Acknowledgements

The authors are acknowledging the financial support received from the University Research Fellowship (Grant Number: URF-PU/A&A-3/URF/2014) in the year 2014–2016 from Periyar University, Salem, India. The authors are thankful to Sejong University, Republic of Korea, for their support.

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Authors and Affiliations

  1. Department of Environmental Science, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636 011, India

    D. Kalaimurugan, K. Durairaj, P. Vasudhevan & S. Venkatesan

  2. Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea

    B. Balamuralikrishnan

  3. Department of Biotechnology, School of Biosciences, Periyar University, Salem, Tamil Nadu, 636 011, India

    M. S. Shivakumar

  4. Nutritional Improvement of Crops, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    T. Kaul

  5. Department of Environmental Energy and Engineering, Kyonggi University, Youngtong-Gu, Suwon, Gyeonggi-Do, 16227, South Korea

    S. W. Chang & B. Ravindran

Authors
  1. D. Kalaimurugan
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  2. B. Balamuralikrishnan
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  3. K. Durairaj
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  4. P. Vasudhevan
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  5. M. S. Shivakumar
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  6. T. Kaul
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  7. S. W. Chang
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  8. B. Ravindran
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  9. S. Venkatesan
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Correspondence to S. Venkatesan.

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Editorial responsibility: M. Abbaspour.

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Kalaimurugan, D., Balamuralikrishnan, B., Durairaj, K. et al. Isolation and characterization of heavy-metal-resistant bacteria and their applications in environmental bioremediation. Int. J. Environ. Sci. Technol. 17, 1455–1462 (2020). https://doi.org/10.1007/s13762-019-02563-5

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  • DOI: https://doi.org/10.1007/s13762-019-02563-5

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