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Cr-induced disturbance on expression of six COX genes in rice seedlings

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Abstract

Cytochrome c oxidase (COX) is one of the principle enzymes involved in cell apoptosis in plants, due to oxidative stresses and mitochondrial dysfunction. In this study, hydroponic experiment was conduced to investigate transcriptional changes of six selected COX genes in rice seedlings (Oryza sativa L. cv. XZX 45) exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) using quantitative real-time RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on COX activity were also determined. Results indicated that accumulation of Cr in rice tissues was evident, in which much more Cr was detected in roots rather than shoots from both Cr treatments. Significant increase in COX activity was only observed in shoots at the highest Cr treatment concentration from both Cr variants, whereas changes of COX activity in roots responded biphasically. Six selected target genes of COX1, COX2, COX5B, COX6A, COX6B, and COX7A from the rice COX gene family were expressed differentially in response to both Cr variants. Expression pattern of these selected COX genes was also variable in rice tissues. Information collected here suggests that selected COX genes from the rice COX gene family may play different roles during detoxification of Cr stress.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No: 41761094) and the Guangxi Talent Highland for Hazardous Waste Disposal Industrialization.

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  1. The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541004, People’s Republic of China

    X.-Z. Yu, C.-J. Lu, Y.-J. Lin & Y.-H. Li

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  1. X.-Z. Yu
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Correspondence to X.-Z. Yu.

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Editorial responsibility: M. Abbaspour.

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Yu, XZ., Lu, CJ., Lin, YJ. et al. Cr-induced disturbance on expression of six COX genes in rice seedlings. Int. J. Environ. Sci. Technol. 16, 2385–2394 (2019). https://doi.org/10.1007/s13762-018-1848-y

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  • DOI: https://doi.org/10.1007/s13762-018-1848-y

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