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Developments in taxol production through endophytic fungal biotechnology: a review

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Abstract

Paclitaxel (taxol), a diterpene natural compound was first extracted from the bark of yew trees. However, the method cannot meet its increasing demand on the market due to unprecedented yew cutting, low amounts of taxol production, the laborious and slow process of taxol extraction. Recently, efforts have been made to develop alternative means of taxol production. Microbial fermentation would be a promising method in the production of taxol at industrial scale. Fungal endophytes have the capacity to produce bioactive compounds and can independently synthesize secondary metabolites similar to those made by the host plants. Optimization of the fermentation culture is one of the most important strategies in increasing taxol production by endophytic fungi supplemented with several substances including carbon sources, nitrogen sources, precursors, inducer and the metabolic bypass inhibitors. Improved fermentation techniques and different biotechnological strategies such as gene cloning, gene transformation, mutations are widely being used on endophytic fungi in order to increase the productivity of the taxol. In this review, the different strategies used to produce taxol from endophytic fungal biotechnology have been discussed.

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Acknowledgements

Authors are grateful to Dr. Syed Abrar for his kind help during preparation of the manuscript. Author also thankful to Kuvempu University for providing the laboratory facilities in Department of Applied Botany, University Grants Commission, India for providing financial support to B. Shankar Naik during the research period.

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Authors and Affiliations

  1. Department of P.G. Studies and Research in Applied Botany, Bio-Science Complex, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shimoga District, Karnataka, 577451, India

    B. Shankar Naik

  2. Department of Biology, Govt Science College, Chikmagalur, Karnataka, 577101, India

    B. Shankar Naik

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  1. B. Shankar Naik
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Shankar Naik, B. Developments in taxol production through endophytic fungal biotechnology: a review. Orient Pharm Exp Med 19, 1–13 (2019). https://doi.org/10.1007/s13596-018-0352-8

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