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Wsi18 promoter from wild rice genotype, Oryza nivara, shows enhanced expression under soil water stress in contrast to elite cultivar, IR20

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Abstract

Identification and characterization of plant promoters from wild rice genotypes showing inducible expression under soil water stress (SWS) is desirable for transgene expression to generate stress tolerant rice cultivars. A comparative expression profiling of Wsi18, a group 3 LEA gene, revealed differential response under SWS conditions between modern cultivated rice (IR20) and its wild progenitor (Oryza nivara). Wsi18 promoter from O. nivara showed enhanced inducible expression of the reporter gusA gene, encoding β-glucuronidase, in transgenic rice plants in comparison to similar promoter from IR20. Deletion analysis unravelled the cis-acting regulatory elements minimally required for optimal expression of Wsi18 promoter from O. nivara under SWS condition. This is the first report of characterization of an inducible promoter from a wild rice genotype to drive the gene expression under water stress conditions. The Wsi18 promoter element from the wild rice genotype can be used in future genetic manipulation strategies for the generation of SWS tolerant rice cultivars with improved yield characteristics.

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Abbreviations

SWS:

Soil water stress

LEA:

Late embryogenesis abundant

ABA:

Abscisic acid

BS:

Before stress

AS:

After stress

AR:

After recovery

TF:

Transcription factor

GUS:

β-glucuronidase

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Acknowledgments

Financial assistance from National Agricultural Innovation Project, Indian Council of Agricultural Research (NAIP/ICAR) in terms of grant support to laboratory and fellowship to RK are thankfully acknowledged. The authors extend their sincere gratitude to two anonymous reviewers whose incisive comments have helped to improve the clarity of the manuscript.

Author contribution statement

SKS conceived the research. SKS, RK and AC designed the research. RK, AC, RKB and JB performed the experiments. SKS, AKG, RK and AC analyzed the data. AKG and AB provided valuable scientific inputs during the course of the study. RK and AC wrote the manuscript in consultation with AKG and SKS.

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Authors and Affiliations

  1. Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology-Kharagpur, Kharagpur, 721302, India

    Ranjeet Kaur, Anirban Chakraborty, Rupam Kumar Bhunia, Jagannath Bhattacharyya, Asitava Basu & Soumitra Kumar Sen

  2. Department of Biotechnology, Indian Institute of Technology-Kharagpur, Kharagpur, 721302, India

    Ananta Kumar Ghosh

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  1. Ranjeet Kaur
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  2. Anirban Chakraborty
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  3. Rupam Kumar Bhunia
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Kaur, R., Chakraborty, A., Bhunia, R.K. et al. Wsi18 promoter from wild rice genotype, Oryza nivara, shows enhanced expression under soil water stress in contrast to elite cultivar, IR20 . J. Plant Biochem. Biotechnol. 26, 14–26 (2017). https://doi.org/10.1007/s13562-016-0355-9

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