Abstract
Using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry, we investigated the distribution of the low abundant proteins that are involved in soybean seed development in four wild and 12 cultivated soybean genotypes. We found proteomic variation of low abundant proteins involved in soybean seed metabolism within and between groups. The major proteomic variation among these 16 genotypes was observed in the seed maturation proteins, which consisted of 4–6 protein spots. All genotypes showed 3 spots of sucrose-binding protein except one wild genotype which had 2 spots. Two protein spots were detected for a triacylglycerol (TAG) accumulation factor protein in 13 genotypes, while one spot was detected in one ancestral and one wild genotype, and no spot was detected in one of the wild genotypes. All genotypes showed 3 spots of alcohol dehydrogenase except one Asian landrace and one wild genotype that had only one spot. Minor proteomic variation was detected in 16 genotypes for an elongation factor (EF2) and formate dehydrogenase, with apparent absence of EF2 in one landrace and formate dehydrogenase in another landrace genotype. No differences in the proteomic distribution of dehydrin-like protein, seed biotin-containing protein, glyceraldehyde 3-phosphate dehydrogenase, and fructose bisphosphate aldolase were detected. This information will be useful in selecting genotypes for breeding programs, and forms a baseline to which variation in transgenic varieties can be compared.
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Abbreviations
- IPG:
-
immobilized pH gradient
- 2D-PAGE:
-
2- dimensional polyacrylamide gel electrophoresis
- MS:
-
mass spectrometry
- CHCA:
-
α-Cyanohydroxycinnamic acid
- MALDI-TOF-MS:
-
Matrix-assisted laser desorption/ionization time of flight mass spectrometry
- LC-MS:
-
liquid chromatography mass spectrometry
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Acknowledgements
We thank Dr. J. Slovin for critical review of this manuscript. Funding for this research was provided by ARS project 1275-21000-223-00D. Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable.
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Natarajan, S.S., Xu, C., Garrett, W.M. et al. Assessment of the natural variation of low abundant metabolic proteins in soybean seeds using proteomics. J. Plant Biochem. Biotechnol. 21, 30–37 (2012). https://doi.org/10.1007/s13562-011-0069-y
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DOI: https://doi.org/10.1007/s13562-011-0069-y