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Wound healing applications of sericin/chitosan-capped silver nanoparticles incorporated hydrogel

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Abstract

Microbial contamination in wounds leading to severe sepsis can be treated by silver-based antiseptics. However, frequent application of silver-based antiseptics, staining of skin, burning, and irritation at application site resulted to poor patient compliances. Thus, we formulated sericin- and chitosan-capped silver nanoparticle (S/C-SNP)-loaded hydrogel for accelerated wound healing and antimicrobial properties. The wound healing property of sericin, antibacterial nature of chitosan and silver, and mucoadhesive property of carbopol were utilized in development of novel wound dressing hydrogel to investigate the combined effect of these materials for effective treatment of wounds. The chemical reduction method was successfully employed for the synthesis of SNPs using sericin and chitosan as a capping/reducing agent. The SNPs were characterized by ultraviolet-spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The optimized SNPs were further used for preparation of carbopol hydrogel (0.5, 0.75, and 1.0 % w/v). The prepared hydrogels were characterized for pH, viscosity, and texture analysis. The antimicrobial activity and wound healing activity of the optimized hydrogel (S/C-SNPs G-1) demonstrated higher bactericidal activity and wound closure, as supported by results of histopathology. Hydrogel containing capped SNPs has application in wound healing treatment.

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Acknowledgments

The authors thank Department of Applied Animal Science, BBA University, Lucknow, India for providing cocoons of Bombyx mori.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India

    Jyoti Verma, Jovita Kanoujia, Poonam Parashar, Chandra Bhusan Tripathi & Shubhini A. Saraf

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  1. Jyoti Verma
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  2. Jovita Kanoujia
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  3. Poonam Parashar
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  4. Chandra Bhusan Tripathi
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  5. Shubhini A. Saraf
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Corresponding author

Correspondence to Shubhini A. Saraf.

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The animal experiments were performed with the approval of Institutional Animal Ethical Committee (registration number SDCOP&VS/AH/CPCSEA/01/0026).

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The authors declare that they have no conflict of interest.

Additional information

Jyoti Verma and Jovita Kanoujia have equally contributed in doing the benchwork.

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Verma, J., Kanoujia, J., Parashar, P. et al. Wound healing applications of sericin/chitosan-capped silver nanoparticles incorporated hydrogel. Drug Deliv. and Transl. Res. 7, 77–88 (2017). https://doi.org/10.1007/s13346-016-0322-y

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