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Pharmacokinetic interaction studies of fenugreek with CYP3A substrates cyclosporine and carbamazepine

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Abstract

The present study investigated the effect of fenugreek seed powder on disposition of CYP3A substrates, cyclosporine and carbamazepine. Rabbits were treated with fenugreek seed powder (300 mg/kg p.o.) for 8 days and on 8th day the single dose of cyclosporine (30 mg/kg, p.o.) and carbamazepine (40 mg/kg, p.o.) were administered to the corresponding group after 1 h of fenugreek administration. Blood samples were drawn at several time points and analyzed by using UPLC-MS (cyclosporine) and HPLC (carbamazepine). Pharmacokinetic parameters were calculated by using PKSolver. The present investigation reveals that there was no statistically significant difference between pre- and post-treated pharmacokinetic parameters such as AUC0–t , AUC0–∞, C max, T max, T 1/2, K el, MRT0–∞, V z/F , and Cl/F for cyclosporine and carbamazepine. Two tailed “P” values for all these pharmacokinetic parameters were more than 0.05, indicating insignificant impact of fenugreek treatment on the disposition of cyclosporine and carbamazepine. Further, fenugreek may also not have any significant effect on the functionality of P-glycoprotein as cyclosporine is a substrate to P-glycoprotein. The outcomes of present study suggested that fenugreek may not likely to interfere cyclosporine and carbamazepine pharmacokinetics, when co-administered with these drugs.

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Acknowledgments

This work is supported by the National Plan for Science and Technology (NPST), King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia, under the project no. BIO1046-02-10.

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The authors do not have any declaration of interest.

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Correspondence to Mohd Aftab Alam.

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Al-Jenoobi, F.I., Alam, M.A., Alkharfy, K.M. et al. Pharmacokinetic interaction studies of fenugreek with CYP3A substrates cyclosporine and carbamazepine. Eur J Drug Metab Pharmacokinet 39, 147–153 (2014). https://doi.org/10.1007/s13318-013-0149-6

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  • DOI: https://doi.org/10.1007/s13318-013-0149-6

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