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Identification of differential expressed transcripts in cervical cancer of Mexican patients

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Tumor Biology

Abstract

The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization and Southern blotting. After analyzing 1,800 cDNA clones, we found 198 upregulated genes, 166 downregulated, and no significant change of gene expression in 86 clones (p = 0.005). These results were validated by Northern blot analysis (p = 0.0001) in the identification of 28 overexpressed and 7 downregulated transcripts. We observed a set of genes related to the Notch signaling pathway that may be involved in the transformation of cervical cells and in the development to malignancy. The differentially expressed genes may provide useful information about the molecular mechanisms involved in human cervical carcinoma and as diagnostic markers.

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Acknowledgments

We thank Mrs. Sydney Robertson Jiménez for the English correction of the manuscript.

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Authors and Affiliations

  1. División de Biologia Molecular, Instituto Potosino de Investigación Cientifica y Tecnologica A.C. (IPICYT), San Luis Potosi, Mexico

    Leticia Santos, Ma. Fabiola León-Galván, Erika Nahomy Marino-Marmolejo, Ana Paulina Barba de la Rosa & Antonio De León Rodríguez

  2. Departamento de Inmunología, Facultad de Medicina, UASLP, San Luis Potosí, Mexico

    Roberto González-Amaro

  3. Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Celaya, Guanajuato, Mexico

    Ramón Gerardo Guevara-González

  4. Camino a la Presa San José 2055, Col. Lomas 4a. sección, 78216, San Luis Potosi, San Luis Potosi, Mexico

    Leticia Santos

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  1. Leticia Santos
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Correspondence to Leticia Santos.

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Communicated by Leticia Santos

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Santos, L., León-Galván, M.F., Marino-Marmolejo, E.N. et al. Identification of differential expressed transcripts in cervical cancer of Mexican patients. Tumor Biol. 32, 561–568 (2011). https://doi.org/10.1007/s13277-010-0151-4

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  • DOI: https://doi.org/10.1007/s13277-010-0151-4

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