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External spermine prevents UVA-induced damage of Synechocystis sp. PCC 6803 via increased catalase activity and decreased H2O2 and malonaldehyde levels

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Abstract

Common polyamines, putrescine (Put), spermidine (Spd), and spermine (Spm), are cationic compounds known as beneficial factors for many cellular processes including cell division, proliferation, differentiation, and stress response in all living organisms. Effects of exogenous Spm on the protective responses of Synechocystis sp. PCC 6803 exposed to UVA were investigated. The presence of 0.5 mM Spm in the culture medium significantly reduced cell growth after 60 min under white light condition but protected the cells after growing for 60 min under UVA. The stress-tolerant response of Synechocystis cells represented by the ratio of putrescine/spermidine (Put/Spd) showed about a 6-fold increase after 60 min UVA in the presence of Spm. In addition, those levels of chlorophyll a, carotenoids, and photosynthetic oxygen evolution were increased by Spm supplementation in UVA-treated cells. Exogenous Spm induced the activity of catalase but not superoxide dismutase in cells under UVA treatment. On the other hand, Spm treatment enabled cells to apparently decrease the intracellular free radical H2O2 and malonaldehyde (MDA) levels. Overall results suggested that Spm supplementation could protect Synechocystis sp. PCC 6803 cells via the increase of Put/Spd ratio and the reduction of both H2O2 and MDA levels in conjunction with the induction of catalase activity. Interestingly, UVA-treated cells as compared to non-treated cells with exogenous Spm showed a decrease of Spm with an increase of Put and no change in Spd. This suggested the back conversion of Spm to Spd and finally to Put as cellular mechanism in response to UVA.

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Funding

S.J. received research funds from the Faculty of Science, CU (A1B1-NS), Chulalongkorn University and the Graduate School of Chulalongkorn University provided post-doctoral fellowship to W.B. The work is financially supported by the Commission on Higher Education, Thailand (WCU-013-FW-57), by Chulalongkorn University (RES560530052-FW), and by Thailand Research Fund (IRG 5780008) to A.I.

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  1. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Saowarath Jantaro, Wipawee Baebprasert & Aran Incharoensakdi

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  1. Saowarath Jantaro
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  2. Wipawee Baebprasert
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  3. Aran Incharoensakdi
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Correspondence to Saowarath Jantaro.

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Jantaro, S., Baebprasert, W. & Incharoensakdi, A. External spermine prevents UVA-induced damage of Synechocystis sp. PCC 6803 via increased catalase activity and decreased H2O2 and malonaldehyde levels. Ann Microbiol 68, 697–704 (2018). https://doi.org/10.1007/s13213-018-1376-5

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  • DOI: https://doi.org/10.1007/s13213-018-1376-5

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