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α-Lipoic acid nanocapsules fortified cow milk application as a dietary supplement product for anemia

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Abstract

Anemia is an iron deficiency disease caused by the lack of red blood cells to carry sufficient oxygen to the body tissues. Lipoic acid is naturally occurring organosulfur compound with antioxidant property had chosen for the present work to maintain the red blood cell counts through its redox mechanism. The current study aimed to develop lipoic acid nanocapsules (LANCPs) fortified A2 cow milk, thereby this product may act as a natural nutritional supplement. Based on the concept of oil-lipid core with polymeric shell to improve the solubility of the hydrophobic drug. The polymeric surfactant poloxamer 188 increase the LA solubility, coconut oil act as a natural stabilizer and the emulsifier egg lecithin enhanced the stability of the formulation. By varying the concentrations of lipoic acid and poloxamer 188 different trials were performed. The results of FT-IR analysis exhibited the compatibility nature of LA, poloxamer 188, coconut oil, and egg lecithin. The morphology of the developed formulation was observed to be spherically arranged core shell with size ranging from 243.7 ± 9.46 to 394.6 ± 4.05 nm with a surface charge of − 8.04 ± 0.48 to − 1.8 ± 0.5 mV. Results of DPPH assay indicate that the antioxidant property of LANCPs-FM gets maintained even after fortification compared to LANCPs. RP-HPLC analysis showed the presence of LA in LANCPs-FM. In-vitro release of LA from LANCPs-FM showed that 96.28 ± 2.35% of LA were sustained release from LANCPs-FM at 4 h. The results of in-vivo studies in anemia induced rats showed that the blood counts (RBC and Hb) of LANCPs-FM supplemented rats gets increased compared to CM/LA-CM. This suggests that the developed LANCPs might be effective for the treatment of anemia progression especially for patients undergoing hemodialysis.

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Acknowledgements

The authors gratefully acknowledge the Department of Science and Technology (GoI), New Delhi supported National Facility for Drug Development for Academia, Pharmaceutical and Allied Industries (NFDD) (Ref No. VI-D&P/349/10-11/TDT/1 Dt: 21.10.2010) and National Facility for Bioactive Peptides from Milk (NFBP) Project (Ref No. VI- D&P/545/2016-17/TDT; Dt: 28.02.2017). We acknowledge the HRTEM FACILITY at SRMIST set up with support from MNRE (Project No. 31/03/2014-15/PVSE-R&D), Government of India. The authors acknowledge Madras Pharma, Chennai for providing gift drug sample of Alpha lipoic acid.

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  1. Centre for Excellence in Nanobio Translational Research (CENTRE), Department of Pharmaceutical Technology, University College of Engineering, Anna University, BIT Campus, Tiruchirappalli, Tamilnadu, 620 024, India

    Azeera Mohamed Saliq, Venkateshwaran Krishnaswami, Kumar Janakiraman & Ruckmani Kandasamy

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  1. Azeera Mohamed Saliq
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  2. Venkateshwaran Krishnaswami
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Mohamed Saliq, A., Krishnaswami, V., Janakiraman, K. et al. α-Lipoic acid nanocapsules fortified cow milk application as a dietary supplement product for anemia. Appl Nanosci 10, 2007–2023 (2020). https://doi.org/10.1007/s13204-020-01304-2

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