Abstract
Nowadays, nanomaterials that are smaller than 100 nm in size are very attractive owing to their enhanced physicochemical properties. Although they have been used widely for industrial applications, their toxicity still remains a problem. This article is a new record of the synthesis of titanium dioxide nanoparticles (TiO2 NPs) by a Mentha aquatica leaf extract and determination of its toxicity to rat marrow mesenchymal stem cells. In this study, we aimed to determine the genotoxic and cytotoxic effects of biologically synthetized TiO2 NPs. The characteristic peak of the nanomaterial was observed at 354 nm. The mean size of the nanomaterial was measured to be 69 nm from SEM images. According to zeta analysis, the surface charge of the nanomaterial was − 37.6 mV. The crystalline structure of the nanomaterial was determined using XRD analysis. It was concluded that the obtained nanomaterial was TiO2 The results of the FT-IR analysis showed that the functional groups that were found in the plant extract could play an important role in the formation and stabilization of TiO2 NPs. The effective size of the TiO2 NPs was found to be 304 nm using DLS analysis. The TGA analysis results showed that the total mass loss was 4% at 900 °C. According to DNA cleavage analysis results, TiO2 NPs cause damage to the plasmid pBR322 DNA in a concentration-dependant matter. It has been noted that TiO2 NPs lead to decreased cell viability during increased time and concentration of applications on rat marrow mesenchymal stem cells. It has also been determined that bulk TiO2 causes a greater reduction in the stem cell viability compared to the biosynthesized NPs. The obtained results could be useful for further application and toxicity studies.
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References
Acosta-Torres LS, Nuñez-Anita RE, Vanegas-Lancón RD, de la Fuente J, López-Marín LM, Castaño VM (2013) Nanoengineering of dental materials: applications to prosthetics. Recent Patents Nanomed 3:2–8. https://doi.org/10.2174/1877912311303010002
An H, Jin B (2012) Prospects of nanoparticle–DNA binding and its implications in medical biotechnology. Biotechnol Adv 30(6):1721–1732. https://doi.org/10.1016/j.biotechadv.2012.03.007
Armendariz V, Herrera I, Peralta-Videa JR, Jose-Yacaman M, Troiani H, Santiago P, Gardea-Torresdey LL (2004) Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology. J Nanopart Res 6:377–382. https://doi.org/10.1007/s11051-004-0741-4
Chellappa M, Anjaneyulu U, Manivasagam G, Vijayalakshmi U (2015) Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method. Int J Nanomed 10:31–41. https://doi.org/10.2147/IJN.S79978
Conforti F, Ioele G, Statti GA, Marrelli M, Ragno G, Menichini F (2008) Antiproliferative activity against human tumor cell lines and toxicity test on Mediterranean dietary plants. Food Chem Toxicol 46(10):3325–3332. https://doi.org/10.1016/j.fct.2008.08.004
Duman F, Ocsoy I, Ozturk F (2016) Chamomile flower extract-directed CuO nanoparticle formation for its antioxidant and DNA cleavage properties. Mater Sci Eng C 60:333–338. https://doi.org/10.1016/j.msec.2015.11.052
Fu PP, Xia Q, Hwang H, Ray PC, Yu H (2014) Mechanisms of nanotoxicity: generation of reactive oxygen species. J Food Drug Anal 22(1):64–75. https://doi.org/10.1016/j.jfda.2014.01
Gorbe M, Bhat R, Aznar E, Sancenon F, Marcos MD, Herraiz FJ, Prohens J, Venkataraman A, Martínez-Máñez R (2016) Rapid biosynthesis of silver nanoparticles using pepino (Solanum muricatum) leaf extract and their cytotoxicity on HeLa cells. Materials 9(5):1–15. https://doi.org/10.1111/j.1750-3841.2012.02944.x
Harshiny M, Iswarya CN, Matheswaran M (2015) Biogenic synthesis of iron nanoparticles using Amaranthus dubius leaf extract as a reducing agent. Powder Technol 286:744–749. https://doi.org/10.1016/j.powtec.2015.09.021
Iswarya V, Bhuvaneshwari M, Alex SA, Iyer S, Chaudhuri G, Chandrasekaran PT, Bhalerao GM, Chakravarty S, Raichur AM, Chandrasekaran N, Mukherjee A (2015) Combined toxicity of two crystalline phases (anatase and rutile) of titania nanoparticles towards freshwater microalgae: Chlorella sp. Aquat Toxicol 161:154–169. https://doi.org/10.1016/j.aquatox
Jamuna KS, Banu S, Brindha P, Kurian GA (2014) Nano-scale preparation of titanium dioxide by Desmodium gangeticum root aqueous extract. Ceram Int 40:11933–11940. https://doi.org/10.1016/j.ceramint.2014.04.029
Jin C, Zhu BS, Wang X, Lu Q (2008) Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Chem Res Toxicol 21(9):1871–1877. https://doi.org/10.1021/tx800179f
Karatoprak GŞ, Aydın G, Altınsoy B, Altınkaynak C, Koşar M, Ocsoy I (2017) The effect of Pelargonium endlicherianum Fenzl. root extracts on formation of nanoparticles and their antimicrobial activities. Enzyme Microbial Technol 97:21–26. https://doi.org/10.1016/j.enzmictec.2016.10.019
Kharissova OV, Dias HVR, Kharisov BI, Perez BO, Perez VMJ (2013) The greener synthesis of nanoparticles. Trends Biotechnol 31(4):240–248. https://doi.org/10.1016/j.tibtech.2013.01.003
Kirthi AV, Rahuman AA, Rajakumar G, Marimuthu S, Santhoshkumar T, Jayaseelan T, Elango G, Zahir AA, Kamaraj C, Bagavan A (2011) Biosynthesis of titanium dioxide nanoparticles using bacterium Bacillus subtilis. Mater Lett 65(17–18):2745–2747. https://doi.org/10.1016/j.matlet.2011.05.077
Krishnasamy A, Sundaresan M, Velan P (2015) Rapid phytosynthesis of nano-sized titanium using leaf extract of Azadirachta indica. Int J Chem Tech Res 8(4):2047–2052
Kucková L, Jomová K, Švorcová A, Valko M, Segľa P, Monco J, Kožíšek J (2015) Synthesis, crystal structure, spectroscopic properties and potential biological activities of salicylate-neocuproine ternary copper(II) complexes. Molecules 20:2115–2137. https://doi.org/10.3390/molecules20022115
Kumar PS, Balachandran C, Duraipandiyan V, Ramasamy D, Ignacimuthu S, Al-Dhabi NA (2015) Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties. Appl Nanosci 5:169–180. https://doi.org/10.1007/s13204-014-0304-7
Kushwaha HB, Malik CP (2012) Nanofabrication of silver nanoparticles from the stem and leaf extract of Verbesina encelioides. Natl Acad Sci Lett 35(6):555–563. https://doi.org/10.1007/s40009-012-0097-8
Makumire S, Chakravadhanula VS, Köllisch G, Redel E, Shonhai A (2014) Immunomodulatory activity of zinc peroxide (ZnO2) and titanium dioxide (TiO2) nanoparticles and their effects on DNA and protein integrity. Toxicol Lett 227(1):56–64. https://doi.org/10.1016/j.toxlet.2014.02.027
Malarkodi C, Chitra K, Rajeshkumar S, Gnanajobitha G, Paulkumar K, Vanaja M, Annadurai G (2013) Novel eco-friendly synthesis of titanium oxide nanoparticles by using Planomicrobium sp. and its antimicrobial evaluation. Pelagia Res Library 4(3):59–66
Manke A, Wang L, Rojanasakul Y (2013) Mechanisms of nanoparticle-ınduced oxidative stress and toxicity. Biomed Res Int 2013:1–15. https://doi.org/10.1155/2013/942916
Ocsoy I, Paret ML, Ocsoy MA, Kunwar S, Chen T, You M, Tan W (2013) Nanotechnology in plant disease management: dna-directed silver nanoparticles on graphene oxide as an antibacterial against Xanthomonas perforans. ACS Nano 7:8973–8980. https://doi.org/10.1021/nn4034794
Ocsoy I, Demirbaş A, McLamore ES, Altınsoy B, Ildız N, Baldemir A (2017) Green synthesis with incorporated hydrothermal approaches for silver nanoparticles formation and enhanced antimicrobial activity against bacterial and fungal pathogens. J Mol Liq 238:263–269. https://doi.org/10.1016/j.molliq.2017.05.012
Roopan SM, Bharathi A, Prabhakarn A, Rahuman AA, Velayutham K, Rajakumar G, Padmaja RD, Lekshmi M, Madhumitha G (2012) Efficient phyto-synthesis and structural characterization of rutile TiO2 nanoparticles using Annona squamosa peel extract. Spectrochim Acta Part A Mol Biomol Spectrosc 98:86–90. https://doi.org/10.1016/j.saa.2012.08.055
Sahni C, Panwar A, Kaur B (2015) Controlled green synthesis of silver nanoparticles by Allium cepa and Musa acuminata with strong antimicrobial activity. Int Nano Lett 5:93–100. https://doi.org/10.1007/s40089-015-0142-y
Salam HA, Sivaraj R (2014) Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae mediated green synthesis and characterization of titanium dioxide nanoparticles. Adv Biores 5(3):10–16
Sangeetha G, Rajeshwari S, Venckatesh R (2011) Green synthesis of zinc oxide nanoparticles by Aloe barbadensis miller leaf extract: structure and optical properties. Mater Res Bull 46:2560–2566. https://doi.org/10.1016/j.materresbull.2011.07.046
Sankar R, Manikandan P, Malarvizhi V, Fathima T, Shivashangari KS, Ravikumar V (2014) Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation. Spectrochim Acta Part A Mol Biomol Spectrosc 121:746–750. https://doi.org/10.1016/j.saa.2013.12.020
Sankar R, Rizwana K, Shivashangari KS, Ravikumar V (2015) Ultra-rapid photocatalytic activity of Azadirachta indica engineered colloidal titanium dioxide nanoparticles. Appl Nanosci 5(6):731–736. https://doi.org/10.1007/s13204-014-0369-3
Santhoshkumar T, Rahuman AA, Jayaseelan C, Rajakumar G, Marimuthu S, Kirthi AV, Velayutham K, Thomas J, Venkatesan J, Kim S (2014) Green synthesis of titanium dioxide nanoparticles using Psidium guajava extract and its antibacterial and antioxidant properties. Asian Pac J Trop Med 2014:968–976. https://doi.org/10.1016/S1995-7645(14)60171-1
Sha B, Gao W, Wang S, Xu F, Lu T (2011) Cytotoxicity of titanium dioxide nanoparticles differs in four liver cells from human and rat. Compos B 42(8):2136–2144. https://doi.org/10.1016/j.compositesb.2011.05.009
Shah M, Fawcett D, Sharma S, Tripathy SK, Poinern GEJ (2015) Green synthesis of metallic nanoparticles via biological entities. Materials 8(11):7278–7308. https://doi.org/10.3390/ma8115377
Shende S, Ingle AP, Gade A, Rai M (2015) Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity. World J Microbiol Biotechnol 31:865–873. https://doi.org/10.1007/s11274-015-1840-3
Sivaraj R, Rahman PKSM, Rajiv P, Narendhran S, Venckatesh R (2014) Biosynthesis and characterization of Acalypha indica mediated copper oxide nanoparticles and evaluation of its antimicrobial and anticancer activity. Spectrochim Acta Part A Mol Biomol Spectrosc 129:255–258. https://doi.org/10.1016/j.saa.2014.03.027
Sivaranjani V, Philominathan P (2016) Synthesize of titanium dioxide nanoparticles using Moringa oleifera leaves and evaluation of wound healing activity. Wound Med 12:1–5. https://doi.org/10.1016/j.wndm.2015.11.002
Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79–84. https://doi.org/10.1007/s00449-008-0224-6
Valli DrG, Geetha S (2015) A green method for the synthesis of titanium dioxide using Cas sia auriculata leaves extract. Eur J Biomed Pharm Sci 2(3):490–497
Velayutham K, Rahuman AA, Rajakumar G, Santhoshkumar T, Marimuthu S, Jayaseelan C, Bagavan A, Kirthi AV, Kamaraj C, Zahir AA, Elango G (2012) Evaluation of Catharanthus roseus leaf extract-mediated biosynthesis of titanium dioxide nanoparticles against Hippobosca maculata and Bovicola ovis. Parasitol Res 111(6):2329–2337. https://doi.org/10.1007/s00436-011-2676-x
Wang Y, Cui H, Zhou J, Li F, Wang J, Chen M, Liu Q (2015) Cytotoxicity, DNA damage, and apoptosis induced by titanium dioxide nanoparticles in human non-small cell lung cancer A549 cells. Environ Sci Pollut Res 22(7):5519–5530. https://doi.org/10.1007/s11356-014-3717-7
Acknowledgements
This study was supported by Erciyes University Scientific Research Projects (BAP) unit FDK-2015-5650. We thank Dilek Bahar at Erciyes University Betul-Ziya Eren Genome and Stem Cell Center for assistance with the cytotoxic activity analysis.
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Koca, F.D., Duman, F. Genotoxic and cytotoxic activity of green synthesized TiO2 nanoparticles. Appl Nanosci 9, 815–823 (2019). https://doi.org/10.1007/s13204-018-0712-1
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DOI: https://doi.org/10.1007/s13204-018-0712-1