Skip to main content
Log in

A rapid molecular method to detect the invasive golden apple snail Pomacea canaliculata (Lamarck, 1822)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The golden apple snail, Pomacea canaliculata has become a major agricultural and environmental pest across Asia. Here, using the mitochondrial cytochrome oxidase I as a diagnostic, we develop a multiplex PCR, that discriminates P. canaliculata from the most common non-invasive Pomacea species, thereby providing a fast and reliable diagnostic tool.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Anderson B (1993) The Philippines snail disaster. Ecologist 23:70–72

    Google Scholar 

  • Carlsson NOL, Bronmark C, Hansson L (2004) Invading herbivory: the golden apple snail alters ecosystem functioning in Asian wetlands. Ecology 85:1575–1580

    Article  Google Scholar 

  • Cowie RH (2002) Apple snails (Ampullariidae) as agricultural pests: their biology, impacts and management. In: Barker GM (ed) Molluscs as crop pests. CAB International, New York

    Google Scholar 

  • Estebenet AL, Martin PR (2003) Shell interpopulation variation and its origin in Pomacea canaliculata (Gastropoda: Ampullariidae) from southern pampas, Argentina. J Molluscan Stud 69:301–310

    Article  Google Scholar 

  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299

    PubMed  CAS  Google Scholar 

  • GenBank® National Centre for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA. http://www.ncbi.nlm.nih.gov/Genbank/

  • Halwart M (1994) The golden apple snail (Pomacea canaliculata) in Asian rice farming systems: present impact and future threat. Int J Pest Manage 40:199–206

    Article  Google Scholar 

  • Herbert PDN, Cywinksa A, Ball SL, DeWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc B Biol Sci 270:313–321

    Article  Google Scholar 

  • Hirai Y (1988) Apple snail in Japan—the present status and management. Japan Agricult Res Quart 22:161–165

    Google Scholar 

  • Matienzo LH (1984) Wilson Ang’s big foot snails. Greenfields 14:24–29

    Google Scholar 

  • Matsukura K, Okuda M, Kubota K, Wada T (2008) Genetic divergence of the genus Pomacea (Gastropoda: Ampullariidae) distributed in Japan, and a simple molecular method to distinguish P. canaliculata and P. insularum. Appl Entomol Zoo 43:535–540

    Article  CAS  Google Scholar 

  • Mochida O (1991) Spread of freshwater Pomacea snails (Pilidae, Mollusca) from Argentina to Asia. Micronesia Suppl 3:51–62

    Google Scholar 

  • Pain T (1946) On Pila canaliculata and its locality. Proceed Malacological Soc 27:58–59

    Google Scholar 

  • Rejesus JM, Sayshoc AS, Joshi RC (1990) The distribution and control of the introduced golden apple snail (Pomacea sp.) in the Philippines. In: Introduction of germplasm and quarantine procedure. PLANTI: Kula Lumpur, Malaysia, pp 213–224

Download references

Acknowledgments

This project was funded by Australian Government Department of Agriculture, Fisheries and Forestry Training. The authors thank Jacek Plazinski (Plant Biosecurity, DAFF), John MacDonald and Wayne Burcher (AQIS) for their support in the project. Matthias Glaubrecht for providing samples of Pomacea from SE Asia.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. N. Johnson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cooke, G.M., King, A.G., Miller, L. et al. A rapid molecular method to detect the invasive golden apple snail Pomacea canaliculata (Lamarck, 1822). Conservation Genet Resour 4, 591–593 (2012). https://doi.org/10.1007/s12686-011-9599-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9599-9

Keywords

Navigation