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Biosynthesis of Silver Nanoparticles by Methylophilus quaylei, Characterization and Its Impact on Established Biofilms

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Abstract

In this study, Methylophilus quaylei proved to possess the ability to reduce AgNO3 and produce silver nanoparticles (AgNPs) under specific conditions via bacterial cells and cell-free supernatant (CFS). The obtained AgNPs via bacterial cells were examined by the transmission of electron microscopy (TEM) consistent with X-ray microanalysis and for AgNPs via CFS by visible (UV–Vis) absorption spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The purpose of this study was to evaluate the antibiofilm effect of AgNPs. The effectiveness of the minimum inhibitory concentration (MIC) of AgNPs in supplementation with CFS was determined on established biofilms on polypropylene by the number of colony forming unit (CFU). These nanoparticles exhibit an antibiofilm effect. CFS was tested individually and in supplementation with the MIC AgNPs obtained on established biofilms of M. quaylei MT and M. quaylei SM. The CFS individually decreased established biofilm for both strains. Further, the obtained MIC of AgNPs in combination with CFS exhibited significant antibiofilm effect against established biofilms for both strains by the destruction of biofilm architecture and detecting cell morphological abnormalities that were observed by SEM. These results showed that the obtained AgNPs had a strong antibiofilm effect against the examined strains.

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Authors and Affiliations

  1. Department of Biotechnology and Industrial Pharmacy, M. V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, Moscow, Russian Federation, 119571

    Abir M. H. A. Mohamed & Anna B. Pshenichnikova

  2. Agricultural Microbiology Department, Faculty of Agriculture, Fayoum University, 63514, Faiyum, Egypt

    Abir M. H. A. Mohamed

  3. Federal State Institution, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow, Russian Federation

    Vladimir V. Sorokin & Dmitry A. Skladnev

  4. Gamaleya Federal Research Centre for Microbiology and Immunology of the Ministry of Public Health, Moscow, Russian Federation

    Natalia V. Shevlyagina & Vladimir G. Zhukhovitsky

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  1. Abir M. H. A. Mohamed
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  2. Vladimir V. Sorokin
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Mohamed, A.M.H.A., Sorokin, V.V., Skladnev, D.A. et al. Biosynthesis of Silver Nanoparticles by Methylophilus quaylei, Characterization and Its Impact on Established Biofilms. BioNanoSci. 10, 885–898 (2020). https://doi.org/10.1007/s12668-020-00780-8

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