Abstract
Cold stress has been shown to induce the production of reactive oxygen species (ROS), which can elicit a potentially damaging oxidative burden on cellular metabolism. Here, the expression of a metallothionein gene (AtMT2a) was upregulated under low temperature and hydrogen peroxide (H2O2) stresses. The Arabidopsis T-DNA insertion mutant, mt2a, exhibited more sensitivity to cold stress compared to WT plants during the seed germination, and H2O2 levels in mt2a mutant were higher than that in WT plants during the cold stress. Synthetic GFP fused to AtMT2a was observed to be localized in cytosol. These results indicated that AtMT2a functions in tolerance against cold stress by mediating the ROS balance in the cytosol. Interestingly, mRNA level of AtMT2a was increased in seedlings of Arabidopsis cat2 mutant after cold treatment compared to WT seedlings, and overexpression of AtMT2a in cat2 could improve CAT activity under chilling stress. Moreover, the enzymatic activity of CAT in mt2a was higher than that in WT plants after cold treatment, suggesting that AtMT2a and CAT might complement each other in antioxidative process potentially in Arabidopsis. Taken together, our results provided a novel insight into the relationship between MTs and antioxidative enzymes in the ROS-scavenging system in plants.
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Acknowledgements
We thank Dr. J. Haseloff (MRC Laboratory of Molecular Biology, Cambridge, UK) for the GFP construction pBINmGFP5-ER. This work was supported by the National Basic Research Program (Grant No. 2006CB1001006), the National Natural Science Foundation (Grants 30970230), and the National Special Program for Research and Industrialization of Transgenic Plants (Grant No. 2009ZX08009-092B) in China.
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Zhu, W., Zhao, DX., Miao, Q. et al. Arabidopsis thaliana Metallothionein, AtMT2a, Mediates ROS Balance during Oxidative Stress. J. Plant Biol. 52, 585–592 (2009). https://doi.org/10.1007/s12374-009-9076-0
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DOI: https://doi.org/10.1007/s12374-009-9076-0