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Nitrogen-Fixing and Plant Growth-Promoting Ability of Two Endophytic Bacterial Strains Isolated from Sugarcane Stalks

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Abstract

Two N2-fixing bacteria isolates, B11S and B8S, were obtained from the surface-sterilized stalks of sugarcane cultivars GT11 and B8. Both isolates showed acetylene reduction activity in nitrogen-free media. The two nitrogen-fixing bacterial isolates produced siderophores, and the ability of B8Ss was higher than that of B11S. The two isolates were also able to dissolve Ca3(PO4)2, and the ability of B11S was higher. It was found that only the strain B8S displayed ACC deaminase activity. The IAA production ability of strain B8S was higher than that of B11S. Both the B11S and B8S isolates were classified on the basis of 16 S rRNA gene sequences and their biological characteristics. The 16 S rRNA gene sequence of isolates B11S and B8S showed 98.5 and 100 % similarities with Stenotrophomonas maltophilia and Agrobacterium tumefaciens (Rhizobium radiobacter), respectively. For both growth and nitrogenase activity of the isolates, the optimum temperature was 31 °C. The optimum pH for their growth was 6.0, but it ranged from 6.5 to 7.0 for nitrogenase activity. The two strains grew faster and showed increased nitrogenase activity with increasing concentration of carbon in the cultural media, for which the sucrose was better compared to glucose. Further, the growth and nitrogenase activity of the strains were found better when cultured in the media containing suitable concentrations of NH4 + and NO3 −; however, the growth of both isolates was hindered significantly with increasing concentration of both nitrogen sources.

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Acknowledgments

The present study was supported by the National High Technology Research and Development Program (“863” Program) of China (2013AA102604), National Natural Science Foundation of China (31101122, 31171504, 31471449), International Scientific Exchange Program projects (2013DFA31600), Guangxi Special Funds for Bagui Scholars and Distinguished Experts, Guangxi R & D Research Program projects (Gui Ke Chan 1123008-1, Gui Ke Gong 1222009-1B, Gui Ke He 1347004-2) and Doctoral Start-up Project of Guangxi University (X160059).

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Authors and Affiliations

  1. State Key Laboratory for Subtropical Agri-Bioresources Conservation and Utilization, College of Agriculture, Guangxi University, Nanning, 530004, Guangxi, China

    Yong-Xiu Xing, Chun-Yan Wei, Yao Mo, Li-Tao Yang & Yang-Rui Li

  2. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Yong-Xiu Xing, Li-Tao Yang, Si-Liang Huang & Yang-Rui Li

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  1. Yong-Xiu Xing
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  2. Chun-Yan Wei
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Correspondence to Li-Tao Yang or Yang-Rui Li.

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Xing, YX., Wei, CY., Mo, Y. et al. Nitrogen-Fixing and Plant Growth-Promoting Ability of Two Endophytic Bacterial Strains Isolated from Sugarcane Stalks. Sugar Tech 18, 373–379 (2016). https://doi.org/10.1007/s12355-015-0397-7

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  • DOI: https://doi.org/10.1007/s12355-015-0397-7

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