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Characterization of siderophore produced by Pseudomonas syringae BAF.1 and its inhibitory effects on spore germination and mycelium morphology of Fusarium oxysporum

  • Microbial Physiology and Biochemistry
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Abstract

In this study, an antagonistic bacterium against Fusarium oxysporum was identified and designated as Pseudomonas syringae strain BAF.1 on the basis of 16S rDNA sequence analysis and physiological-biochemical characteristics. It produced catechol-species siderophore at a molecular weight of 488.59 Da and a maximum amount of 55.27 μg/ml with glucose as a carbon source and asparagine as a nitrogen source at a C/N ratio of 10:1, 30°C and pH 7. The siderophore exhibited prominent antagonistic activity against Fusarium oxysporum with a maximum inhibition rate of 95.24% and had also suppressive effects on other kinds of 11 phytopathogenic fungi in the absence of FeCl3·6H2O. Spore germination was completely inhibited by 50 μl of the siderophorecontaining solution, and the ultrastructures of mycelia and spores were also considerably suppressed by siderophore treatment as established by electron microscopy observation. These results indicate that the siderophore produced by Pseudomonas syringae BAF.1 could be potentially used for biocontrol of pathogenic Fusarium oxysporum.

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Authors and Affiliations

  1. College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, P. R. China

    Sumei Yu, Chunying Teng, Jinsong Liang, Tao Song, Liying Dong, Xin Bai, Yu Jin & Juanjuan Qu

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  1. Sumei Yu
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  2. Chunying Teng
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  3. Jinsong Liang
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  4. Tao Song
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  5. Liying Dong
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  6. Xin Bai
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  7. Yu Jin
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  8. Juanjuan Qu
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Correspondence to Juanjuan Qu.

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Yu, S., Teng, C., Liang, J. et al. Characterization of siderophore produced by Pseudomonas syringae BAF.1 and its inhibitory effects on spore germination and mycelium morphology of Fusarium oxysporum. J Microbiol. 55, 877–884 (2017). https://doi.org/10.1007/s12275-017-7191-z

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  • DOI: https://doi.org/10.1007/s12275-017-7191-z

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