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Preparation of Chlorophyll-free Young Barley Leaf Extract Powders Using Supercritical Carbon Dioxide Modified with Cosolvent

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  • Bioprocess Engineering
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Abstract

In this study, we attempted to prepare chlorophyll-free young barley leaf extract powders using three different supercritical carbon dioxide (SCCO2) drying processes. The pure SCCO2 drying process for a methanolic extract solution resulted in a ~30% increase in the antioxidant (saponarin and isovitexin) content and a ~75% decrease in the chlorophyll content compared to a solvent evaporation method. The drying process with ethanol-modified SCCO2 increased the antioxidant content considerably and completely removed the chlorophylls. However, this process could not dry an aqueous ethanolic extract solution with a more than 30% water content. The ethanol-modified SCCO2 drying process that employed a periodic flush with pure SCCO2 was found to effectively dry an aqueous ethanolic extract solution with more than 30% water content.

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Acknowledgements

This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ00950201)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Korea

    Jin-Hee Hong & Seungjoo Haam

  2. Department of Chemical Engineering, The University of Suwon, Suwon, 18323, Korea

    Heaung-Shig Oh, Giobin Lim & Jong-Hoon Ryu

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  1. Jin-Hee Hong
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  2. Seungjoo Haam
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Correspondence to Giobin Lim or Jong-Hoon Ryu.

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Hong, JH., Haam, S., Oh, HS. et al. Preparation of Chlorophyll-free Young Barley Leaf Extract Powders Using Supercritical Carbon Dioxide Modified with Cosolvent. Biotechnol Bioproc E 24, 997–1006 (2019). https://doi.org/10.1007/s12257-019-0236-x

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  • DOI: https://doi.org/10.1007/s12257-019-0236-x

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