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Enhanced In Vitro Antimicrobial Activity of Polymyxin B–Coated Nanostructured Lipid Carrier Containing Dexamethasone Acetate

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Abstract

Purpose

The increase in ocular infections mainly by Pseudomonas aeruginosa is directly related to the emergence and widespread use of contact lenses; Polymyxin B has been used in therapy for treating this ophthalmic pathogen. Recently, nanostructured systems have been proposed to enhance antimicrobial activity of antibiotics.

Methods

In this study, a nanostructured lipid carrier (NLC) containing dexamethasone acetate (DEX-NLC) was developed, and its surface was modified by polymyxin B sulfate (DEX-NLC+PS) with the purpose of the antimicrobial activity enhancement against P. aeruginosa. DEX-NLC was obtained by high-pressure homogenization and coated with PS.

Results

The NLC Z-average was 244.73 ± 7.82 nm; the PI and ZP showed 0.226 ± 0.048 and 2.724 ± 0.458 mV, respectively. The formulation showed adequate stability and physicochemical characteristics. The developed nanosystem enhanced the PS antimicrobial activity 2- to 3-fold against Pseudomonas aeruginosa.

Conclusion

This result suggests that DEX-NLC+PS can offer a promising alternative against Gram-negative bacterial infections accompanied by inflammation.

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Authors and Affiliations

  1. Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil

    Emmily Dantas Rocha, Marcia Regina Spuri Ferreira, Edson dos Santos Neto, Eduardo José Barbosa, Felipe Rebello Lourenço & Nadia Bou-Chacra

  2. Faculty of Pharmacy and Pharmaceutical Science, University of Alberta, Edmonton, T6G 2E1, Canada

    Raimar Löbenberg

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  1. Emmily Dantas Rocha
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Rocha, E.D., Ferreira, M.R.S., dos Santos Neto, E. et al. Enhanced In Vitro Antimicrobial Activity of Polymyxin B–Coated Nanostructured Lipid Carrier Containing Dexamethasone Acetate. J Pharm Innov 16, 125–135 (2021). https://doi.org/10.1007/s12247-020-09427-3

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