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Molecular Markers for Assessing Must Varietal Origin

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Abstract

Wine quality and market value greatly depend on the grapevine varietal composition, which may be characteristic of specific regions. In order to defend the distinct regions, Denominations of Origin were defined to protect against fraudulent practices. In this study, we evaluated the efficiency of two microsatellite-based systems (microsatellite (SSR) and intermicrosatellite (ISSR)) for must varietal composition determination and their potential role in certification purposes. Eleven Vitis vinifera L. varieties from leaf and monovarietal must DNA samples were screened with six SSR and 14 ISSR primers to discriminate polymorphisms. Principal coordinates analysis was performed with DCENTER on the resultant data using unweighted pair group mathematical average and revealed that ISSRs markers were not suitable for certification procedures, whereas nuclear SSR markers presented a complete correspondence between leaf and must samples, demonstrating that they were adequate for traceability purposes.

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Acknowledgements

This work was supported by the project Genomics Applied to Portuguese Wine Traceability (PTDC/AGR-ALI/69516/2006) and a PhD grant to Leonor Pereira (SFRH/BD/44781/2008) from the Fundação para a Ciência e Tecnologia-Portugal.

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Authors and Affiliations

  1. Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (CGB-IBB/UTAD), P.O. Box 1013, 5000-911, Vila Real, Portugal

    Leonor Pereira, Paula Martins-Lopes, Cláudia Batista & Henrique Guedes-Pinto

  2. National Institute of Biological Resources/National Institute of Agrarian Research (INRB/INIA Dois Portos), Quinta d’Almoinha, 2565-191, Dois Portos, Portugal

    Geni C. Zanol, Pedro Clímaco, João Brazão & José E. Eiras-Dias

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  1. Leonor Pereira
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  2. Paula Martins-Lopes
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  3. Cláudia Batista
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  4. Geni C. Zanol
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  8. Henrique Guedes-Pinto
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Correspondence to Paula Martins-Lopes.

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Pereira, L., Martins-Lopes, P., Batista, C. et al. Molecular Markers for Assessing Must Varietal Origin. Food Anal. Methods 5, 1252–1259 (2012). https://doi.org/10.1007/s12161-012-9369-7

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