Abstract
A cytogenetic map was established for the trifoliate orange, Poncirus trifoliata. Chromosome size, centromere position and CMA+ heterochromatin content were determined for each chromosome pair, together with the position of BAC clones previously described as chromosome-specific markers. Although P. trifoliata is a true biological species, heteromorphisms for the size of the heterochromatic bands in two chromosome pairs were observed. A minimum set of four BACs was proposed for chromosome identification in P. trifoliata, as well as a chromosome nomenclature that can be applied to all citrus species. The Ctv locus was assigned to chromosome 9 and the remaining linkage groups can now be integrated to this physical map. This standard nomenclature for trifoliate orange will be valuable for genome-sequencing projects in all citrus species.
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Abbreviations
- BAC:
-
Bacterial artificial chromosome
- DAPI:
-
4′6-diamidino-2-phenylindole
- CMA:
-
Chromomycin A3
- FISH:
-
Fluorescence in situ hybridization
- CTV:
-
Citrus tristeza virus
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Acknowledgements
We thank Getúlio de Souza Vieira (Embrapa Cassava&Fruits) for collecting the seeds. This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Communicated by Paul Moore
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da Costa Silva, S., Marques, A., dos Santos Soares Filho, W. et al. The Cytogenetic Map of the Poncirus trifoliata (L.) Raf.—A Nomenclature System for Chromosomes of All Citric Species. Tropical Plant Biol. 4, 99–105 (2011). https://doi.org/10.1007/s12042-011-9072-7
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DOI: https://doi.org/10.1007/s12042-011-9072-7