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Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2

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Abstract

Our previous study has showed that co-grafted Schwann cells (SCs) promote proliferation and migration of the grafted oligodendrocyte precursor cells (OPCs). However, how the co-grafted SCs affect OPCs has not been clarified. In the present study, we confirmed that SC-induced proliferation and migration of OPCs were mediated by SC-secreted factors using SC-conditioned medium (SCM). Then, we detected several candidate factors, PDGF-AA, FGF-2, and IGF-1, in SCs and SCM, and their receptors in OPCs. Finally, by using the selective inhibitors, the effects of these candidate factors on proliferation and migration of OPCs were examined. Our results showed that SCM-stimulated proliferation and migration of OPCs could be markedly decreased by both AG1295 (the inhibitor of PDGFR) and PD173074 (the inhibitor of FGFR). Together, our study suggests that SCs affect proliferation and migration of OPCs through secreting PDGF-AA and FGF-2. Identity of these molecules not only contributes to understand the mechanism of SC-induced proliferation and migration of OPCs but also provides possible target for treatment of CNS diseases.

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References

  • Acheson A, Barker PA, Alderson RF, Miller FD, Murphy RA (1991) Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: inhibition by antibodies to NGF. Neuron 7:265–275

    Article  CAS  PubMed  Google Scholar 

  • Banai S, Wolf Y, Golomb G, Pearle A, Waltenberger J, Fishbein I, Schneider A, Gazit A, Perez L, Huber R, Lazarovichi G, Rabinovich L, Levitzki A, Gertz SD (1998) PDGF-receptor tyrosine kinase blocker AG1295 selectively attenuates smooth muscle cell growth in vitro and reduces neointimal formation after balloon angioplasty in swine. Circulation 97:1960–1969

    Article  CAS  PubMed  Google Scholar 

  • Bandtlow CE, Heumann R, Schwab ME, Thoenen H (1987) Cellular localization of nerve growth factor synthesis by in situ hybridization. EMBO J 6:891–899

    CAS  PubMed Central  PubMed  Google Scholar 

  • Baumann N, Pham-Dinh D (2001) Biology of oligodendrocyte and myelin in the mammalian central nervous system. Physiol Rev 81:871–927

    CAS  PubMed  Google Scholar 

  • Cai F, Campana WM, Tomlinson DR, Fernyhough P (1999) Transforming growth factor-beta1 and glial growth factor 2 reduce neurotrophin-3 mRNA expression in cultured Schwann cells via a cAMP-dependent pathway. Brain Res Mol Brain Res 71:256–264

    Article  CAS  PubMed  Google Scholar 

  • Chau CH, Shum DK, Li H, Pei J, Lui YY, Wirthlin L, Chan YS, Xu XM (2004) Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury. FASEB J Off Publ Fed Am Soc Exp Biol 18:194–196

    CAS  Google Scholar 

  • Cui QL, Almazan G (2007) IGF-I-induced oligodendrocyte progenitor proliferation requires PI3K/Akt, MEK/ERK, and Src-like tyrosine kinases. J Neurochem 100:1480–1493

    CAS  PubMed  Google Scholar 

  • Ding Y, Yan Q, Ruan JW, Zhang YQ, Li WJ, Zeng X, Huang SF, Zhang YJ, Wang S, Dong H, Zeng YS (2011) Bone marrow mesenchymal stem cells and electroacupuncture downregulate the inhibitor molecules and promote the axonal regeneration in the transected spinal cord of rats. Cell Transplant 20:475–491

    Article  PubMed  Google Scholar 

  • Feron F, Perry C, Cochrane J, Licina P, Nowitzke A, Urquhart S, Geraghty T, Mackay-Sim A (2005) Autologous olfactory ensheathing cell transplantation in human spinal cord injury. Brain J Neurol 128:2951–2960

    Article  CAS  Google Scholar 

  • Fok-Seang J, Mathews GA, FFrench-Constant C, Trotter J, Fawcett JW (1995) Migration of oligodendrocyte precursors on astrocytes and meningeal cells. Dev Biol 171:1–15

    Article  CAS  PubMed  Google Scholar 

  • Givogri MI, Galbiati F, Fasano S, Amadio S, Perani L, Superchi D, Morana P, Del Carro U, Marchesini S, Brambilla R, Wrabetz L, Bongarzone E (2006) Oligodendroglial progenitor cell therapy limits central neurological deficits in mice with metachromatic leukodystrophy. J Neurosci Off J Soc Neurosci 26:3109–3119

    Article  CAS  Google Scholar 

  • Harrison B (1985) Schwann cell and oligodendrocyte remyelination in lysolecithin-induced lesions in irradiated rat spinal cord. J Neurol Sci 67:143–159

    Article  CAS  PubMed  Google Scholar 

  • Hu JG, Fu SL, Zhang KH, Li Y, Yin L, Lu PH, Xu XM (2004) Differential gene expression in neural stem cells and oligodendrocyte precursor cells: a cDNA microarray analysis. J Neurosci Res 78:637–646

    Article  CAS  PubMed  Google Scholar 

  • Hu J, Deng L, Wang X, Xu XM (2009) Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro. J Neurosci Res 87:2854–2862

    Article  CAS  PubMed  Google Scholar 

  • Hu JG, Wang XF, Deng LX, Liu NK, Gao X, Chen JH, Zhou FC, Xu XM (2013) Cotransplantation of glial restricted precursor cells and Schwann cells promotes functional recovery after spinal cord injury. Cell Transplant 22:2219–2236

    Article  PubMed  Google Scholar 

  • Kodelja V, Muller C, Tenorio S, Schebesch C, Orfanos CE, Goerdt S (1997) Differences in angiogenic potential of classically vs alternatively activated macrophages. Immunobiology 197:478–493

    Article  CAS  PubMed  Google Scholar 

  • Lee JC, Mayer-Proschel M, Rao MS (2000) Gliogenesis in the central nervous system. Glia 30:105–121

    Article  CAS  PubMed  Google Scholar 

  • Luk F, Yu Y, Walsh WR, Yang JL (2011) IGF1R-targeted therapy and its enhancement of doxorubicin chemosensitivity in human osteosarcoma cell lines. Cancer Invest 29:521–532

    Article  CAS  PubMed  Google Scholar 

  • Ma Z, Cao Q, Zhang L, Hu J, Howard RM, Lu P, Whittemore SR, Xu XM (2009) Oligodendrocyte precursor cells differentially expressing Nogo-A but not MAG are more permissive to neurite outgrowth than mature oligodendrocytes. Exp Neurol 217:184–196

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ma YH, Zhang Y, Cao L, Su JC, Wang ZW, Xu AB, Zhang SC (2010) Effect of neurotrophin-3 genetically modified olfactory ensheathing cells transplantation on spinal cord injury. Cell Transplant 19:167–177

    Article  PubMed  Google Scholar 

  • McDermott U, Ames RY, Iafrate AJ, Maheswaran S, Stubbs H, Greninger P, McCutcheon K, Milano R, Tam A, Lee DY, Lucien L, Brannigan BW, Ulkus LE, Ma XJ, Erlander MG, Haber DA, Sharma SV, Settleman J (2009) Ligand-dependent platelet-derived growth factor receptor (PDGFR)-alpha activation sensitizes rare lung cancer and sarcoma cells to PDGFR kinase inhibitors. Cancer Res 69:3937–3946

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Milner R, Anderson HJ, Rippon RF, McKay JS, Franklin RJ, Marchionni MA, Reynolds R, Ffrench-Constant C (1997) Contrasting effects of mitogenic growth factors on oligodendrocyte precursor cell migration. Glia 19:85–90

    Article  CAS  PubMed  Google Scholar 

  • Miyamoto Y, Yamauchi J, Tanoue A (2008) Cdk5 phosphorylation of WAVE2 regulates oligodendrocyte precursor cell migration through nonreceptor tyrosine kinase Fyn. J Neurosci Off J Soc Neurosc 28:8326–8337

    Article  CAS  Google Scholar 

  • Morrissey TK, Kleitman N, Bunge RP (1991) Isolation and functional characterization of Schwann cells derived from adult peripheral nerve. J Neurosci Off J Soc Neurosci 11:2433–2442

    CAS  Google Scholar 

  • Neuberger TJ, De Vries GH (1993) Distribution of fibroblast growth factor in cultured dorsal root ganglion neurons and Schwann cells. I Localization during maturation in vitro. J Neurocytol 22:436–448

    Article  CAS  PubMed  Google Scholar 

  • Oudega M, Xu XM (2006) Schwann cell transplantation for repair of the adult spinal cord. J Neurotrauma 23:453–467

    Article  PubMed  Google Scholar 

  • Pardo OE, Latigo J, Jeffery RE, Nye E, Poulsom R, Spencer-Dene B, Lemoine NR, Stamp GW, Aboagye EO, Seckl MJ (2009) The fibroblast growth factor receptor inhibitor PD173074 blocks small cell lung cancer growth in vitro and in vivo. Cancer Res 69:8645–8651

    Article  CAS  PubMed  Google Scholar 

  • Raff MC, Miller RH, Noble M (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303:390–396

    Article  CAS  PubMed  Google Scholar 

  • Rao MS, Mayer-Proschel M (2000) Precursor cells for transplantation. Prog Brain Res 128:273–292

    Article  CAS  PubMed  Google Scholar 

  • Springer JE, Mu X, Bergmann LW, Trojanowski JQ (1994) Expression of GDNF mRNA in rat and human nervous tissue. Exp Neurol 127:167–170

    Article  CAS  PubMed  Google Scholar 

  • Vora P, Pillai PP, Zhu W, Mustapha J, Namaka MP, Frost EE (2011) Differential effects of growth factors on oligodendrocyte progenitor migration. Eur J Cell Biol 90:649–656

    Article  CAS  PubMed  Google Scholar 

  • Xu XM, Martin GF (1989) Developmental plasticity of the rubrospinal tract in the North American opossum. J Comp Neurol 279:368–381

    Article  CAS  PubMed  Google Scholar 

  • Xu XM, Onifer SM (2009) Transplantation-mediated strategies to promote axonal regeneration following spinal cord injury. Respir Physiol Neurobiol 169:171–182

    Article  PubMed Central  PubMed  Google Scholar 

  • Xu XM, Guenard V, Kleitman N, Bunge MB (1995) Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J Comp Neurol 351:145–160

    Article  CAS  PubMed  Google Scholar 

  • Xu XM, Chen A, Guenard V, Kleitman N, Bunge MB (1997) Bridging Schwann cell transplants promote axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J Neurocytol 26:1–16

    Article  CAS  PubMed  Google Scholar 

  • Zha J, Smith A, Andreansky S, Bracchi-Ricard V, Bethea JR (2014) Chronic thoracic spinal cord injury impairs CD8+ T-cell function by up-regulating programmed cell death-1 expression. J Neuroinflammation 11:65

    Article  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (nos. 81171465 and 81471277).

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    Authors and Affiliations

    1. Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      Yue-Juan Chen, Jing-Xing Zhang, Zheng-Rong Zhong & Jian-Guo Hu

    2. Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      Yue-Juan Chen, Jing-Xing Zhang, Lin Shen, Qi Qi, Zheng-Rong Zhong, Zhi-Quan Jiang, Rui Wang, He-Zuo Lü & Jian-Guo Hu

    3. Laboratory of Cell Biology and Molecular Genetics, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, People’s Republic of China

      Xiao-Xin Cheng

    4. Central Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      He-Zuo Lü

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    1. Yue-Juan Chen
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    Correspondence to He-Zuo Lü or Jian-Guo Hu.

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    Yue-Juan Chen and Jing-Xing Zhang contributed equally to this work.

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    Chen, YJ., Zhang, JX., Shen, L. et al. Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2. J Mol Neurosci 56, 999–1008 (2015). https://doi.org/10.1007/s12031-015-0570-1

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    • DOI: https://doi.org/10.1007/s12031-015-0570-1

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