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Seasonal Variability of Elemental Composition and Phytochemical Analysis of Moringa oleifera Leaves Using Energy-Dispersive X-ray Fluorescence and Other Related Methods

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Abstract

The elemental contents and the phytochemical components of Moringa oleifera are crucial for nutrition and medical purposes. Therefore, the monthly and seasonal variations of the elemental composition and phytochemical analysis of Moringa oleifera leaves collected from the same ecological area have been investigated. For this purpose, Moringa oleifera leaves were collected monthly from the same tree’s branches during the whole year from January 2019 to December 2019. A non-destructive elemental analysis technique was used, namely energy-dispersive X-ray fluorescence spectrometry. The seasonal variations are different from one season to another. The highest concentrations of Mo, Cr, Fe, Ti, and Si were found during winter, whereas the highest concentrations of Br, Cl, and Cu were found during the summer seasons. Based on Pearson’s correlation analysis, a strong correlation between Ca and Sr was found, whereas Sr has a negative correlation with other detected elements. Similarly, Cu and Zn as well as Br and Cl have a strong correlation. Remarkable different concentrations were found during May which has the lowest positive correlation. The phytochemical analysis revealed that Moringa oleifera leaves collected during the spring season resulted in the highest chlorophyll content, phenol content, and the greatest scavenging activity. Therefore, the mineral contents and phytochemical compounds are affected by the changing of the seasons of the year. Collectively, the current results are useful for optimizing the harvest time of Moringa oleifera leaves with respect to the quality.

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Funding

Dr. Fahmy A. S. Hassan acknowledges Taif University Researchers Supporting Project number (TURSP-2020/143), Taif University, Taif, Saudi Arabia.

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Authors and Affiliations

  1. Spectroscopy Department, Physics Division, National Research Centre, El Behooth Str., 12622 Dokki, Cairo, Egypt

    Abdallah A. Shaltout

  2. Department of Biology, College of Science, Taif University, P. O. Box 11099, Taif, 21944, Saudi Arabia

    Fahmy A. S. Hassan

  3. Horticulture Department, Faculty of Agriculture, Tanta University, Tanta, Egypt

    Fahmy A. S. Hassan

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  1. Abdallah A. Shaltout
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Correspondence to Abdallah A. Shaltout.

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Shaltout, A.A., Hassan, F.A.S. Seasonal Variability of Elemental Composition and Phytochemical Analysis of Moringa oleifera Leaves Using Energy-Dispersive X-ray Fluorescence and Other Related Methods. Biol Trace Elem Res 199, 4319–4329 (2021). https://doi.org/10.1007/s12011-020-02523-y

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