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Synergistic Effects of Dietary Selenomethionine and Vitamin C on the Immunity, Antioxidant Status, and Intestinal Microbiota in Sea Cucumber (Apostichopus japonicus)

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Abstract

A 30-day feeding trial was carried out to investigate the interactive effects of dietary selenium (selenomethionine) and vitamin C (Vc) in Apostichopus japonicus. Two selenium (0 and 5 mg/kg) and three vitamin C (0, 5000, and 10,000 mg/kg) combined groups of feed were formulated (Designated as LSeLVc, LSeMVc, LSeHVc, HSeLVc, HSeMVc and HSeHVc, respectively) and fed the sea cucumbers. Our results showed no significant effects on the growth-related parameters in sea cucumber (P > 0.05). Furthermore, the reciprocal action between Se and Vc had significant (P < 0.05) effects on Se accumulation in the respiratory tree and intestines. Also, the lysozyme, glutathione peroxidase activity, and the relative expression levels such as LZM, GPX, Hsp70, and Hsp90 in different tissues were significantly increased in the group of sea cucumber fed diet with 5 mg Se in combination with 5000 mg Vc compared with the control group (P < 0.05). However, MDA and H2O2 contents in the body wall were significantly reduced in the HSeHVc group (P < 0.05). In addition, analysis of intestinal flora revealed that Haloferula abundance was highest in the LSeMVc group than other treatment groups, and Vibrio abundance was decreased with combined Se and Vc supplement. Finally, the species diversity of the gut microbial community of sea cucumber in HSeMVc group was lower than those in other treatment groups. The results showed that the interaction of selenium and vitamin C had positive effects on improving the immune status, antioxidant capacity, and digestive ability of A. japonicus.

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Acknowledgments

Authors express their gratitude to Prof. Dr. Tongjun Ren, Dr. Yuzhe Han, and Mr. Lianshun Wang who supported the management and plan of this experiment. We thank Zhuoan Bai, Jing Zhou, Zhengzheng Bi, and Anran Dong for their contribution to the laboratory works. Finally, thanks to Md Hasim Rabbi and Yanan Hu for their help on writing and editing English language text.

Funding

This work is supported by the grants from National Oceanic Administration (No. 201405003) and Liaoning marine industrial technology research institute (2018-CY-30).

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  1. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China

    Fanshuang Zeng, Md Hasim Rabbi, Yanan Hu, Zequn Li, Xue Ren, Yuzhe Han & Tongjun Ren

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  1. Fanshuang Zeng
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Zeng, F., Rabbi, M.H., Hu, Y. et al. Synergistic Effects of Dietary Selenomethionine and Vitamin C on the Immunity, Antioxidant Status, and Intestinal Microbiota in Sea Cucumber (Apostichopus japonicus). Biol Trace Elem Res 199, 3905–3917 (2021). https://doi.org/10.1007/s12011-020-02483-3

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