Skip to main content

Advertisement

SpringerLink
Log in

Investigations of Human Fascia Lata Elemental Composition—the Effect of Different Preservation and Mineralisation Methods

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Influence of fixation medium and storage conditions as well as impact of sample mineralisation procedure on determination of minerals in human fascia lata was examined and discussed. Freezing and storage in 10% neutral buffered formalin solution and in 2.5% glutaraldehyde were used as the preservation methods of the samples. The concentrations of, both essential and toxic, elements were measured by ICP-OES method in fascia lata samples mineralised with concentrated nitric acid in a closed microwave system and in open vessels heated on a hot plate. Freezing was found as the best preserving method of fascia lata samples because of the number of elements determined and determination precision. The trace element (Cd, Cr, Cu, Fe, Ni, Sr, Zn) concentrations obtained in samples decomposed using the conventional hot plate were different from analogous measurements in solutions obtained after application of closed vessels and microwave energy assistance. Differences between the mineral compositions of fascia samples variously preserved and mineralised were statistically evaluated and discussed. Interelement correlations were analysed taking into account an impact of various methods of sample conservation. Strong, positive association between element content was discovered for Cr-Ba, Mn-Ba, P-Ba, Sr-Ba, Sr-Ca, Zn-Ca, Mn-Cr, Pb-Cr, Sr-Cr, Mg-Fe, P-Fe, Pb-Ni, Ti-Ni and Sr-P pairs of elements.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Organ Donation and Transplantation Activities (2014) http://www.transplant-observatory.org; Web site designed, developed, and maintained by the Spanish National Transplant Organization (ONT) in collaboration with World Health Organization (WHO)

  2. Konkov AS, Pustovalova OL, Agapov II (2010) Biocompatible materials from regenerated silk for tissue engineering and medicinal therapy. Appl Biochem Microbiol 46(8):739–744

    Article  CAS  Google Scholar 

  3. Olender E, Uhrynowska-Tyszkiewicz I, Kaminski A (2011) Revitalization of biostatic tissue allografts: new perspectives in tissue transplantology. Transplant Proc 43(8):3137–3141. https://doi.org/10.1016/j.transproceed.2011.08.069

    Article  CAS  PubMed  Google Scholar 

  4. Stecco C, Macchi V, Porzionato A, Duparc F, De Caro R (2011) The fascia: the forgotten structure. Ital J Anat Embryol 3:127–138 Review

    Google Scholar 

  5. Simmonds N, Miller P, Gemmell H (2012) A theoretical framework for the role of fascia in manual therapy. J Bodywork Mov Therapies 16(1):83–93

    Article  Google Scholar 

  6. Stecco C, Tiengo C, Stecco A, Porzionato A, Macchi V, Stern R, De Caro R (2013) Fascia redefined: anatomical features and technical relevance in fascial flap surgery. Surg Radiol Anat 35(5):369–376. https://doi.org/10.1007/s00276-012-1058-0

    Article  PubMed  Google Scholar 

  7. Stecco C, Pavan P, Pachera P, De Caro R, Natali A (2014) Investigation of the mechanical properties of the human crural fascia and their possible clinical implications. Surg Radiol Anat 36(1):25–32

    Article  Google Scholar 

  8. Bordoni B, Zanier E (2014) Clinical and symptomatological reflections: the fascial system. J Multidiscip Healthc 7:401–411

    Article  Google Scholar 

  9. Blasi M, Blasi J, Domingo T, Pérez-Bellmunt A, Miguel-Pérez M (2015) Anatomical and histological study of human deep fasciae development. Surg Radiol Anat 37:571–578

    Article  Google Scholar 

  10. Dawidowicz J, Szotek S, Matysiak N, Mielańczyk Ł, Maksymowicz K (2015) Electron microscopy of human fascia lata: focus on telocytes. J Cell Mol Med 19(10):2500–2506. https://doi.org/10.1111/JCMM12665/epdf

    Article  PubMed  PubMed Central  Google Scholar 

  11. Dawidowicz J, Matysiak N, Szotek S, Maksymowicz K (2016) Telocytes of fascial structures. Adv Exp Med Biol 913:403–424. https://doi.org/10.1007/978-981-10-1061-3_26

    Article  CAS  PubMed  Google Scholar 

  12. Szotek S, Dawidowicz J, Eyden B, Matysiak N, Czogalla A, Dudzik G, Leśniewicz A, Maksymowicz K (2016) Morphological features of fascia lata in relation to fascia diseases. Ultrastruct Pathol 40:297–310. https://doi.org/10.1080/01913123.2016.1239665

    Article  PubMed  Google Scholar 

  13. Sclafani AP, McCormick SA, Cocker R (2002) Biophysical and microscopic analysis of homologous dermal and fascial materials for facial aesthetic and reconstructive uses. Arch Facial Plast Surg 4(3):164–171

    Article  Google Scholar 

  14. Sikka RS, Neault M, Guanche CA (2005) Reconstruction of the pectoralis major tendon with fascia lata allograft. Orthopedics 28(10):1199–1201

    PubMed  Google Scholar 

  15. Terzis JK, Olivares FS (2008) Uses of the superficial temporal fascial flap in facial paralysis. Plast Reconstr Surg 122(6):176e–185e. https://doi.org/10.1097/PRS.0b013e31818dc012

    Article  PubMed  Google Scholar 

  16. Collar RM, Zopf D, Brown D, Fung K, Kim J (2012) The versatility of the temporoparietal fascia flap in head and neck reconstruction. J Plast Reconstr Aesthet Surg 65(2):141–148. https://doi.org/10.1016/j.bjps.2011.05.00

    Article  PubMed  Google Scholar 

  17. Elias D, Honoré C, Dumont F, Goéré D (2014) Autologous peritoneo-fascial graft: a technique for vascular reconstruction. J Visc Surg 151(6):461–464

    Article  CAS  Google Scholar 

  18. Ishikura H, Fukui N, Takamure H, Ohashi S, Iwasawa M, Takagi K, Horita A, Saito I, Mori T (2015) Successful treatment of a fracture of a huge Achilles tendon ossification with autologous hamstring tendon graft and gastrocnemius fascia flap: a case report. BMC Musculoskelet Disord Open Access 16(1):24. https://doi.org/10.1186/s12891-015-0821-x

    Article  CAS  Google Scholar 

  19. Miyamoto Y, Watanabe M, Ishimoto T, Baba Y, Iwagami S, Sakamoto Y, Yoshida N, Masuguchi S, Ihn H, Baba H (2015) Fascia lata onlay patch for repairing infected incisional hernias. Surg Today 45(1):121–124

    Article  Google Scholar 

  20. Gondo M, Matsumura H, Watanabe K (2017) Clinical application of subdermal areolar tissue and superficial fascia graft as a new material for coverage of small exposure of bone cortex or orthopaedic fixation device. J Plast Surg Hand Surg 51(4):223–227

    Article  Google Scholar 

  21. Zeng L, Jiang C, Li N, Liu W, Wang F, Guo F (2017) Vascularized fascia lata for prevention of postoperative parotid fistula arising from partial parotidectomy during neck dissection. J Oral Maxillofac Surg 75(5):1071–1080

    Article  Google Scholar 

  22. Rose EH (2005) Autogenous fascia lata grafts: clinical applications in reanimation of the totally or partially paralyzed face. Plast Reconstr Surg 116(1):20–32 33-35

    Article  CAS  Google Scholar 

  23. Abuzayed B, Kafadar AM, Oğuzoğlu SA, Canbaz B, Kaynar MY (2009) Duraplasty using autologous fascia lata reenforced by on-site pedicled muscle flap: technical note. J Craniofac Surg 20(2):435–438

    Article  Google Scholar 

  24. Kargi E, Yeşilli C, Akduman B, Babucçu O, Hoşnuter M, Mungan A (2003) Fascia lata grafts for closure of secondary urethral fistulas. Urology 62(5):928–931

    Article  Google Scholar 

  25. Hersh D, Martin FJ, Rowe N (2006) Comparison of silastic and banked fascia lata in pediatric frontalis suspension. J Pediatr Ophthalmol Strabismus 43(4):212–218

    PubMed  Google Scholar 

  26. Cheng Y, Li ZQ, Huang JZ, Xue F, Jiang MJ, Wu KM, Wang QP (2009) Combination of autologous fascia lata and fat injection into the vocal fold via the cricothyroid gap for unilateral vocal fold paralysis. Arch Otolaryngol Head Neck Surg 135(8):759–763. https://doi.org/10.1001/archoto.2009.91

    Article  PubMed  Google Scholar 

  27. Windhofer C, Karlbauer A, Papp C (2009) Bone, tendon, and soft tissue reconstruction in one stage with the composite tensor fascia lata flap. Ann Plast Surg 62(6):665–668. https://doi.org/10.1097/SAP.0b013e31817e9c0f

    Article  CAS  PubMed  Google Scholar 

  28. Hubel A, Spindler R, Skubitz APN (2014) Storage of human biospecimens: selection of the optimal storage temperature. Biopreserv and Biobank 12(3):165–175

    Article  Google Scholar 

  29. Rahil-Khazen R, Botann BJ, Myking A, Ulvik R (2002) Multi-element analysis of trace element levels in human autopsy tissues by using inductively coupled atomic emission spectrometry technique (ICP-AES). J Trace Elem Med Biol 16:15–25

    Article  CAS  Google Scholar 

  30. Unhale SA, Skubitz APN, Solomon R, Hubel A (2012) Stabilization of tissue specimens for pathological examination and biomedical research. Biopreserv and Biobank 10(6):493–500

    Article  Google Scholar 

  31. Ozaki S, Kawase I, Yamashita H, Uchida S, Nozawa Y, Takatoh M, Hagiwara S (2014) A total of 404 cases of aortic valve reconstruction with glutaraldehydedehyde-treated autologous pericardium. J Thorac Cardiovasc Surg 147(1):301–306. https://doi.org/10.1016/j.jtcvs.2012.11.012

    Article  PubMed  Google Scholar 

  32. Figueiredo RL, Silva Dantas MS, Oréfice RL (2011) Thermal welding of biological tissues derived from porcine aorta for manufacturing bioprosthetic cardiac valves. Biotechnol Lett 33:1699–1703. https://doi.org/10.1007/s10529-011-0605-9

    Article  CAS  PubMed  Google Scholar 

  33. Uekusa K, Hayashida M, Ohno Y (2015) Forensic toxicological analyses of drugs in tissues in formalin solutions and in fixatives. Forensic Sci Int 249:165–172. https://doi.org/10.1016/j.forsciint.2015.01.028

    Article  CAS  PubMed  Google Scholar 

  34. Hammer N, Voigt CH, Werner M, Hoffmann F, Bente K, Kunze H, Scholz R, Steinke H (2014) Ethanol and formaldehyde fixation irreversibly alter bones’ organic matrix. J Mech Behav Biomed Mater 29:252–258

    Article  CAS  Google Scholar 

  35. Hamdana L, Bost M, Chazot G, Bui-Xuan B, Vaillant F, Dehina L, Descotes J, Tabib A, Mamou Z, Timour Q (2012) Involvement of neuroleptic drugs in selenium deficiency and sudden death of cardiac origin: study and human post-mortem examination. J Trace Elem Med Biol 26:170–173

    Article  Google Scholar 

  36. Gellein K, Flaten TP, Erikson KM, Aschner M, Syversen T (2008) Leaching of trace elements from biological tissue by formalin fixation. Biol Trace Elem Res 121:221–225

    Article  CAS  Google Scholar 

  37. Leśniewicz A, Furtak M, Żyrnicki W, Dawidowicz J, Maksymowicz K, Szotek S (2014) Mineral composition of human fascia lata. Biol Trace Elem Res 159:440–444

    Article  Google Scholar 

  38. Maksymowicz K, Marycz K, Szotek S, Kaliński K, Serwa E, Łukomski R, Czogała J (2012) Chemical composition of human and canine fascia lata. Acta Biochim Pol 59(4):531–535

    CAS  PubMed  Google Scholar 

  39. Kirilova-Doneva M, Pashkouleva D, Kavardzhikov V (2016) The effects of strain amplitude and localization on viscoelastic mechanical behaviour of human abdominal fascia. Acta Bioeng Biomech 18(4):127–133

    PubMed  Google Scholar 

  40. Schleip R (2010) Biomechanical properties of fascial tissues and their role as pain generators. J Musculoskelet Pain 18(4):393–395

    Article  Google Scholar 

Download references

Funding

The work was financed by a statutory activity subsidy from the Polish Ministry of Science and Higher Education for the Faculty of Chemistry of Wrocław University of Science and Technology and by National Science Centre grant no. N N518 286540.

Author information

Authors and Affiliations

  1. Analytical Chemistry and Chemical Metallurgy Division, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże St. Wyspiańskiego 27, 50-370, Wrocław, Poland

    Anna Leśniewicz, Milena Furtak & Wiesław Żyrnicki

  2. Veterinary Clinic “Brynów”, Brynowska 25c, 40-585, Katowice, Poland

    Joanna Dawidowicz

  3. Faculty of Medicine, Department of Forensic Medicine, Wrocław Medical University, J. Mikulicza-Radeckiego 4, 50-368, Wrocław, Poland

    Krzysztof Maksymowicz

  4. Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Faculty of Mechanical Engineering, Wrocław University of Science and Technology, Łukasiewicza 7/9, 50-371, Wrocław, Poland

    Sylwia Szotek

Authors
  1. Anna Leśniewicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Milena Furtak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wiesław Żyrnicki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joanna Dawidowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Krzysztof Maksymowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sylwia Szotek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anna Leśniewicz.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This study was approved by the Bioethics Committee of Wroclaw Medical University (KB-262/2010).

Statement of Human Rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Bioethics Committee of Wroclaw Medical University and with the 1964 Helsinki declaration and its later amendments.

Additional information

In memory of our friend and mentor, Wiesław Żyrnicki, who passed away on July 4, 2017.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Leśniewicz, A., Furtak, M., Żyrnicki, W. et al. Investigations of Human Fascia Lata Elemental Composition—the Effect of Different Preservation and Mineralisation Methods. Biol Trace Elem Res 187, 357–366 (2019). https://doi.org/10.1007/s12011-018-1389-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-018-1389-8

Keywords

Search

Navigation