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Effects of Cadmium, Chromium and Lead on Growth, Metal Uptake and Antioxidative Capacity in Typha angustifolia

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Abstract

This study investigates the modulation of antioxidant defence system of Typha angustifolia after 30 days exposure of 1 mM chromium (Cr), cadmium (Cd), or lead (Pb). T. angustifolia showed high tolerance to heavy metal toxicity with no visual toxic symptom when exposed to metal stress, and Cd/Pb addition also increased plant height and biomass especially in Pb treatment. Along with increased Cr, Cd, and Pb uptake in metal treatments, there was enhanced uptake of plant nutrients including Ca and Fe, and Zn in Pb treatment. A significant increase in malondialdehyde (MDA) content and superoxide dismutase (SOD) and peroxidase (POD) activities were recorded in plants subjected to Cr, Cd, or Pb stress. Furthermore, Pb stress also improved catalase (CAT), ascorbate peroxidase (APX), and glutathione peroxidase (GPX) activities; whereas Cr stress depressed APX and GPX. The results indicate that enzymatic antioxidants and Ca/Fe uptake were important for heavy metal detoxification in T. angustifolia, stimulated antioxidative enzymes, and Ca, Fe, and Zn uptake could partially explain its hyper-Pb tolerance.

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References

  1. Chen F, Wu FB, Dong J, Vincze E, Zhang G, Wang F, Huang Y, Wei K (2007) Cadmium translocation and accumulation in developing barley grains. Planta 227:223–232

    Article  PubMed  CAS  Google Scholar 

  2. Pilon-Smits E (2005) Phytoremediation. Annu Rev Plant Biol 56:15–39

    Article  PubMed  CAS  Google Scholar 

  3. Wu FB, Dong J, Qian QQ, Zhang GP (2005) Subcellular distribution and chemical form of Cd and Cd–Zn interaction in different barley genotypes. Chemosphere 60:1437–1446

    Article  PubMed  CAS  Google Scholar 

  4. Mulligan CN, Yong RN, Gibbs BF (2001) Remediation technologies for metal-contaminated soil and groundwater: an evaluation. Environ Geol 60:193–207

    Google Scholar 

  5. Järup L, Berglund M, Elinder CG, Nordberg G, Vahter M (1998) Health effects of cadmium exposure — a review of the literature and a risk estimate. Scand J Work Environ Health 24:1–51

    PubMed  Google Scholar 

  6. Davies B, Wixson B (eds) (1988) Lead in soil: issues and guidelines, environmental geochemistry and health monograph series 4, supplement to volume 9 of environmental geochemistry and health. Science Reviews, Northwood, UK

    Google Scholar 

  7. Sinha S, Rai UN, Tripathi RD, Chandra P (1993) Chromium and manganese uptake by Hydrilla verticillata (l.f.) Royle: amelioration of chromium toxicity by manganese. J Environ Sci Health Part A — Toxic/Hazard Subst Environ Eng 28:1545–1552

    Article  Google Scholar 

  8. Chandra P, Sinha S, Rai UN (1997) Bioremediation of Cr from water and soil by vascular aquatic plants. In: Kruger EL, Anderson TA, Coats JR (eds) Phytoremediation of soil and water contaminants (ACS symposium series 664. American Chemical Society, Washington, DC, pp 274–282

    Chapter  Google Scholar 

  9. Muhammad D, Chen F, Zhao J, Zhang GP, Wu FB (2009) Comparison of EDTA and citric acid-enhanced phytoextraction of heavy metals in artificially metal contaminated soil by Typha Angustifolia. Int J Phytorem 11:558–574

    Article  CAS  Google Scholar 

  10. Demirezen D, Aksoy A (2004) Accumulation of heavy metals in Typha angustifolia L. and Potamogeton pectinatus L. living in Sultan Marsh (Kayseri, Turkey). Chemosphere 56:685–696

    Article  PubMed  CAS  Google Scholar 

  11. Dong J, Wu FB, Huang RG, Zhang GP (2007) A chromium-tolerant plant growing in Cr-contaminated land. Int J Phytorem 9:167–179

    Article  CAS  Google Scholar 

  12. Metwally A, Safronova VI, Belimov AA, Dietz KJ (2005) Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J Exp Bot 56:167–178

    PubMed  CAS  Google Scholar 

  13. Pandey V, Dixit V, Shyam R (2005) Antioxidative responses in relation to growth of mustard (Brassica juncea cv. Pusa Jai Kisan) plants exposed to hexavalent chromium. Chemosphere 61:40–47

    Article  PubMed  CAS  Google Scholar 

  14. Cho UH, Seo NH (2005) Oxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation. Plant Sci 168:113–120

    Article  CAS  Google Scholar 

  15. Fu J, Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114

    Article  PubMed  CAS  Google Scholar 

  16. Somashekaraiah BV, Padmaja K, Prasad AK (1992) Phytoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris), involvement of lipid peroxides in chlorophyll degradation. Physiol Plantarum 85:85–89

    Article  CAS  Google Scholar 

  17. Hegeduś A, Erdei S, Horváth G (2001) Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci 160:1085–1093

    Article  PubMed  Google Scholar 

  18. Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A (2001) Cadmium induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in scots pine roots. Plant Physiol 127:887–898

    Article  PubMed  Google Scholar 

  19. Wu FB, Zhang GP, Dominy P (2003) Four barley genotypes respond differently to cadmium: lipid peroxidation and activities of antioxidant capacity. Environ Exp Bot 50:67–78

    Article  CAS  Google Scholar 

  20. Sharma SS, Kaul S, Metwally A, Goyal KC, Finkemeier I, Dietz KJ (2004) Cadmium toxicity to barley (Hordeum vulgare) as affected by varying Fe nutritional status. Plant Sci 166:1287–1295

    Article  CAS  Google Scholar 

  21. Shah K, Kumar R, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161:1135–1144

    Article  CAS  Google Scholar 

  22. Shaw BP (1995) Effects of mercury and cadmium on the activities of antioxidative enzymes in the seedlings of Phaseolus aureus. Biol Plant 37(4):587–596

    Article  CAS  Google Scholar 

  23. Sandalio L, Dalurzo H, Gomez M, Romero-Puertas M, del Rio LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126

    PubMed  CAS  Google Scholar 

  24. Gallego SM, Benavides MP, Tomaro ML (1996) Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci 121:151–159

    Article  CAS  Google Scholar 

  25. Cheng WD, Zhang GP, Yao HG, Dominy P, Wu W, Wang RY (2004) Possibility of predicting heavy-metal contents in rice grains based on DTPA-extracted levels in soil. Commun Soil Sci Plant Anal 35:2731–2745

    Article  CAS  Google Scholar 

  26. Fang R (1991) Application of atomic absorption spectroscopy in sanitary test. Beijing University Press, Beijing, pp 148–158

    Google Scholar 

  27. Zhang XZ (1992) The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system. In: Zhang XZ (ed) Research methodology of crop physiology. Agriculture Press, Beijing, pp 208–211

    Google Scholar 

  28. Ruan HH, Shen WB, Ye MB, Xu LL (2001) Protective effects of nitric oxide on salt stress-induced oxidative damage to wheat (Triticum aestivum L.) leaves. Chinese Sci Bull 46(23):1993–1997

    Google Scholar 

  29. Drotar A, Phelps P, Fall R (1985) Evidence for glutathione peroxidase activities in cultured plant cells. Plant Sci 42:35–40

    Article  CAS  Google Scholar 

  30. Tang Q, Feng MG (1997) Practical statistics and its DPS statistical software package. China Agriculture Press, Bejing

    Google Scholar 

  31. Wójcik M, Vangronsveld J, Tukiendorf A (2005) Cadmium tolerance in Thlaspi caerulescens: I. Growth parameters, metal accumulation and phytochelatin synthesis in response to cadmium. Environ Exp Bot 53:151–161

    Google Scholar 

  32. Lima AIG, Pereira SIA, de Almeida Paula Figueira EM, Caldeira GCN, de Matos Caldeira HDQ (2006) Cadmium detoxification in roots of Pisum sativum seedlings: relationship between toxicity levels, thiol pool alterations and growth. Environ Exp Bot 55:149–162

    Article  CAS  Google Scholar 

  33. Wu FB, Chen F, Wei K, Zhang GP (2004) Effect of cadmium on free amino acid, glutathione and ascorbic acid concentrations in two barley genotypes (Hordeum vulgare L.) differing in cadmium tolerance. Chemosphere 57:447–454

    Article  PubMed  CAS  Google Scholar 

  34. Alkorta I, Hernández-Allica J, Becerril JM, Amezaga I, Albizu I, Garbisu C (2004) Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic. Rev Environ Sci Biotechnol 3:71–90

    Article  CAS  Google Scholar 

  35. Alkorta I, Hernández-Allica J, Becerril JM, Amezaga I, Albizu I, Onaindia M, Garbisu C (2004) Chelate-enhanced phytoremediation of soils polluted with heavy metals. Rev Environ Sci Biotechnol 3:55–70

    Article  CAS  Google Scholar 

  36. Bachir DM, Wu FB, Zhang GP, Wu HX (2004) Genotypic difference in effect of cadmium on development and mineral concentrations of cotton. Commun Soil Sci Plant Anal 35:285–299

    Article  Google Scholar 

  37. Wen ZL, Xiu HN, Mao YF (1999) The utilization and exploitation of cattail plant in environmental protection. Environ Protec 10(39–40):42

    Google Scholar 

  38. Pain SJ (1995) Lead in the environment. Handbook of ecotoxicology. CRC

  39. Brennan MA, Shelley ML (1999) A model of the uptake, translocation and accumulation of lead (Pb) by maize for the purpose of phytoextraction. Ecol Eng 129:271–297

    Article  Google Scholar 

  40. Wojcik M, Vangronsveld J, Tukiendorf A (2005) Cadmium tolerance in Thlaspi caerulescens: I. Growth parameters, metal accumulation and phytochelatin synthesis in response to cadmium. Environ Exp Bot 53:151–161

    CAS  Google Scholar 

  41. Greger M (1999) Metal availability and bioconcentration in plants. In: Prasad MNV, Hagemeyer J (eds) Heavy metal stress in plants (from molecules to ecosystems). Springer, Berlin, pp 1–27

    Google Scholar 

  42. Choudhary M, Jetley UK, Khan MA, Zutshi S, Fatma T (2007) Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotoxicol Environ Saf 66:204–209

    Article  PubMed  CAS  Google Scholar 

  43. Lin CC, Kao CH (2000) Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul 30:151–155

    Article  CAS  Google Scholar 

  44. Baisak RD, Rana PBB, Acharya MK (1994) Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water-stress. Plant Cell Physiol 35:489–495

    CAS  Google Scholar 

  45. Lee MY, Shin HW (2003) Cadmium-induced changes in antioxidant enzymes from the marine alga Nannochloropsis oculata. J Appl Phycol 15:13–19

    Article  CAS  Google Scholar 

  46. Gallego SM, Benavides MP, Tomaro ML (1999) Effect of cadmium ions on antioxidant defense system in sunflower cotyledons. Biol Plant 42(1):49–55

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (30671256). We appreciate Mr. Fei Chen from Agronomy Department of Zhejiang University, for his helpful assistance during the experimental work.

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Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou, 310029, People’s Republic of China

    Alieu Mohamed Bah, Huaxin Dai, Jing Zhao, Hongyan Sun, Fangbin Cao, Guoping Zhang & Feibo Wu

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  1. Alieu Mohamed Bah
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  2. Huaxin Dai
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  4. Hongyan Sun
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  7. Feibo Wu
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Correspondence to Feibo Wu.

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Bah, A.M., Dai, H., Zhao, J. et al. Effects of Cadmium, Chromium and Lead on Growth, Metal Uptake and Antioxidative Capacity in Typha angustifolia . Biol Trace Elem Res 142, 77–92 (2011). https://doi.org/10.1007/s12011-010-8746-6

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