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The Interaction of Chromium(VI) with Urease in Solution

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Abstract

The interaction between K2Cr2O7 and urease was investigated using fluorescence, UV-vis absorption, and circular dichroism (CD) spectroscopy. The experimental results showed that the fluorescence quenching of urease by K2Cr2O7 was a result of the formation of K2Cr2O7–urease complex. The apparent binding constant K A between K2Cr2O7 and urease at 295, 302, and 309 K were obtained to be 2.14 × 104, 1.96 × 104, and 1.92 × 104 L mol−1, respectively. The thermodynamic parameters, ΔH° and ΔS° were estimated to be −5.90 kJ mol−1, 43.67 J mol−1 K−1 according to the Van’t Hoff equation. The electrostatic interaction played a major role in stabilizing the complex. The distance r between donor (urease) and acceptor (K2Cr2O7) was 5.08 nm. The effect of K2Cr2O7 on the conformation of urease was analyzed using UV-vis absorption, CD, synchronous fluorescence spectroscopy, and three-dimensional fluorescence spectra, the environment around Trp and Tyr residues were altered.

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Acknowledgments

The Fund of Excellent Innovation Team of Jiangsu Provincial Department of Education (No. 2009-23), the Natural Science Foundation of Education Department of Jiangsu Province (Grant No. 07KJA18017); the Educational Bureau (Grant No.09KJD150007), Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection (JLCBE09016), the Jiangsu Fundament of “Qilan Project” and “333 Project”, and the Scientific Foundation of Yancheng Teachers University (06YSYJB0205) supported this work.

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Authors and Affiliations

  1. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng City, Jiangsu Province, 224002, People’s Republic of China

    Hong-Mei Zhang & Yan-Qing Wang

  2. Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People’s Republic of China

    Hong-Mei Zhang, Gen-Cheng Zhang & Yan-Qing Wang

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  1. Hong-Mei Zhang
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  2. Gen-Cheng Zhang
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  3. Yan-Qing Wang
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Correspondence to Gen-Cheng Zhang or Yan-Qing Wang.

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Zhang, HM., Zhang, GC. & Wang, YQ. The Interaction of Chromium(VI) with Urease in Solution. Biol Trace Elem Res 141, 53–64 (2011). https://doi.org/10.1007/s12011-010-8718-x

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  • DOI: https://doi.org/10.1007/s12011-010-8718-x

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