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Role of Synthesized Organoselenium Compounds on Protection of Rat Erythrocytes from DMBA-Induced Oxidative Stress

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Abstract

Formation of free radicals is not limited to normal cellular process but also occur upon exposure to certain chemicals (polycyclic aromatic hydrocarbon, cadmium, lead, etc.), cigarette smoke, radiation, and high-fat diet. Free-radical damage is an important factor in many pathological and toxicological processes. Selenium, an essential micronutrient, is a associated with antioxidant functions, physiological defense mechanisms against different diseases including several types of cancers. Search for new selenium compounds with more chemopreventive activities and less toxicities are in progress. In addition, there has been a growing interest in the synthesis of organoselenium compounds with respect to their use in enzymology and bioorganic chemistry. In the present study, adult female Wistar rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA) and the organoselenium compounds [1-isopropyl-3-methylbenzimidazole-2-selenone (Se I) and 1, 3-di-p-methoxybenzylpyrimidine-2-selenone (Se II)] in determined doses. The protective effects of synthetic organoselenium compounds (Se I and Se II) against DMBA-induced changes in antioxidant enzyme (superoxide dismutase, glutathione peroxidase (GSH-Px), catalase (CAT), glutathione reductase (GR)) activities, total GSH, and malondialdehyde (MDA) levels of rat erythrocyte were investigated. The DMBA-treated group exhibited significant decreases in the levels of erythrocyte GSH-Px, CAT, and GR activities, an increase in MDA levels, and a decrease in total GSH level compared to the control. Se I and Se II fully or partially restored enzyme activity. Lipid peroxidation was also decreased in Se-I- and Se-II-treated groups.

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Abbreviations

DMBA:

7,12-dimethylbenz[a]anthracene

PAH:

polycyclic aromatic hydrocarbon

SOD:

superoxide dismutase

GSH-Px:

glutathione peroxidase

CAT:

catalase

GR:

glutathione reductase

Total GSH:

total glutathione

MDA:

malondialdehyde

NADPH:

nicotinamide adenine dinucleotide phosphate

TNB:

5-thio-2-nitrobenzoate

ROS:

reactive oxygen species

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Acknowledgement

We thank Technological and Scientific Research Council of Turkey TUBITAK TBAG-2259 (102T185) for the financial support of this work.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Science, Nigde University, Nigde, 51200, Turkey

    Zeliha Selamoglu Talas

  2. Department of Chemistry, Faculty of Science and Art, Inonu University, 44280, Malatya, Turkey

    Ismet Yilmaz, Ilknur Ozdemir, Burhan Ates & Yetkin Gok

  3. Department of Chemistry, Faculty of Science, Ege University, 35100, Bornova Izmir, Turkey

    Bekir Cetinkaya

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  1. Zeliha Selamoglu Talas
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  2. Ismet Yilmaz
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  6. Bekir Cetinkaya
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Correspondence to Zeliha Selamoglu Talas.

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Selamoglu Talas, Z., Yilmaz, I., Ozdemir, I. et al. Role of Synthesized Organoselenium Compounds on Protection of Rat Erythrocytes from DMBA-Induced Oxidative Stress. Biol Trace Elem Res 128, 167–175 (2009). https://doi.org/10.1007/s12011-008-8262-0

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