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Enhanced Enzymatic Hydrolysis of Palm Pressed Fiber Based on the Three Main Components: Cellulose, Hemicellulose, and Lignin

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Abstract

The enzymatic hydrolysis of the native and the pretreated palm pressed fiber (PPF) was deeply investigated by using the enzyme cocktail ACCELLERASE 1500. Together with the spent PPF from the first hydrolysis and the further doubly-treated PPF, the proportions of three main components were determined and analyzed based on a triangle figure. The proportion (cellulose/hemicelluloses/lignin) in the spent PPF was equal to 44:23:33 and the surface morphology of the spent PPF looks very similar to the native PPF surface showing poor hydrolysis efficiency. After further double treatment, the proportion was changed evidently from the original 44:23:33 to 54:21:25 and the surface structure was significantly disrupted showing a potential to be hydrolyzed completely. Additionally, all samples were characterized by Fourier transform infrared spectroscopy and X-ray diffractogram through considerations of alkaline solution treatment, so as to understand better the nature of biomass hydrolysis, from the aspect of three biomass components.

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Acknowledgments

The authors gratefully acknowledge the financial support of the Sichuan Provincial Science and Technology support program (2013SZ0090), and are grateful for Genencor International Incorporation for gifting the enzyme preparation ACCELLERASE 1500.

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Authors and Affiliations

  1. Department of Environmental Science and Engineering, College of Resource and Environment, Sichuan Agricultural University, Chengdu, 610065, People’s Republic of China

    Lili Lin, Fei Shen, Xiaohong Zhang, Yanzong Zhang & Shihuan Deng

  2. Institute of Environmental Science and Engineering, Nanyang Technological University, Innovation Center, Block 2, Unit 237, 18 Nanyang Drive, Singapore, 637723, Singapore

    Rong Yan

  3. College of Architecture and Environment, Sichuan University, Chengdu, 610065, People’s Republic of China

    Wenju Jiang

  4. College of Agriculture, Sichuan Agricultural University, Chengdu, 610065, People’s Republic of China

    Zhuang Li

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  1. Lili Lin
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Correspondence to Lili Lin.

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Lin, L., Yan, R., Jiang, W. et al. Enhanced Enzymatic Hydrolysis of Palm Pressed Fiber Based on the Three Main Components: Cellulose, Hemicellulose, and Lignin. Appl Biochem Biotechnol 173, 409–420 (2014). https://doi.org/10.1007/s12010-014-0848-8

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  • DOI: https://doi.org/10.1007/s12010-014-0848-8

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