Skip to main content
SpringerLink
Log in

Protective Effect of 3,4-Dihydroxybenzoic Acid Isolated from Cladophora wrightiana Harvey Against Ultraviolet B Radiation-Induced Cell Damage in Human HaCaT Keratinocytes

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The aim of the present study was to elucidate the protective properties of 3,4-dihydroxybenzoic acid (DBA) isolated from Cladophora wrightiana Harvey (a green alga) against ultraviolet B (UVB)-induced damage to human HaCaT keratinocytes. DBA exhibited scavenging actions against the 1,1-diphenyl-2-picrylhydrazyl radical, the superoxide anion, and the hydroxyl radical. Furthermore, DBA decreased the levels of intracellular reactive oxygen species generated by hydrogen peroxide or UVB treatment of the cells. DBA also decreased the UVB-augmented levels of phospho-histone H2A.X and the extent of comet tail formation, which are both indications of DNA damage. In addition, the compound safeguarded keratinocytes from UVB-induced injury by reversing the production of apoptotic bodies, overturning the disruption of mitochondrial membrane potential, increasing the expression of the anti-apoptotic protein, B-cell lymphoma 2, and decreasing the expression of the pro-apoptotic proteins, Bcl-2-associated X and cleaved caspase-3. Taken together, these results demonstrate that DBA isolated from a green alga protects human keratinocytes against UVB-induced oxidative stress and apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Vinayak, R. C., Sudha, S. A., & Chatterji, A. (2011). Journal of the Science of Food and Agriculture, 91, 2471–2476.

    Article  CAS  Google Scholar 

  2. Senthilkumar, P., & Sudha, S. (2011). Jundishapur Journal of Microbiology, 5, 411–415.

    Article  Google Scholar 

  3. Sabeena Farvin, K. H., & Jacobsen, C. (2013). Food Chemistry, 138, 1670–1681.

    Article  CAS  Google Scholar 

  4. Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Free Radical Biology & Medicine, 20, 933–956.

    Article  CAS  Google Scholar 

  5. Andreasen, M. F., Kroon, P. A., Williamson, G., & Garcia-Conesa, M. T. (2001). Free Radical Biology & Medicine, 31, 304–314.

    Article  CAS  Google Scholar 

  6. Yu, L., Haley, S., Perret, J., Harris, M., Wilson, J., & Qian, M. (2002). Journal of Agricultural and Food Chemistry, 50, 1619–1624.

    Article  CAS  Google Scholar 

  7. Yu, L., Perret, J., Harris, M., Wilson, J., & Haley, S. (2003). Journal of Agricultural and Food Chemistry, 51, 1566–1570.

    Article  CAS  Google Scholar 

  8. Hattori, Y., Nishigori, C., Tanaka, T., Uchida, K., Nikaido, O., Osawa, T., et al. (1996). The Journal of Investigative Dermatology, 107, 733–737.

    Article  CAS  Google Scholar 

  9. Ahmed, N. U., Ueda, M., Nikaido, O., Osawa, T., & Ichihashi, M. (1999). The British Journal of Dermatology, 140, 226–231.

    Article  CAS  Google Scholar 

  10. Dinkova-Kostova, A. T. (2008). Planta Medica, 74, 1548–1559.

    Article  CAS  Google Scholar 

  11. Amaral, S., Redmann, K., Sanchez, V., Mallidis, C., Ramalho-Santos, J., & Schlatt, S. (2013). Andrology, 1, 707–714.

    Article  CAS  Google Scholar 

  12. Lee, C. H., Wu, S. B., Hong, C. H., Yu, H. S., & Wei, Y. H. (2013). International Journal of Molecular Sciences, 14, 6414–6435.

    Article  CAS  Google Scholar 

  13. Harvey, W. H. (1860). Proceedings of the American Academy of Arts and Sciences, 4, 327–335.

    Google Scholar 

  14. Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D., & Mitchell, J. B. (1987). Cancer Research, 47, 943–946.

    CAS  Google Scholar 

  15. Ueno, I., Kohno, M., Yoshihira, K., & Hirono, I. (1984). Journal of Pharmacobio-Dynamics, 7, 563–569.

    Article  CAS  Google Scholar 

  16. Kohno, M., Mizuta, Y., Kusai, M., Masumizu, T., & Makino, K. (1994). Bulletin of the Chemical Society of Japan, 67, 1085–1090.

    Article  CAS  Google Scholar 

  17. Li, L., Abe, Y., Mashino, T., Mochizuki, M., & Miyata, N. (2003). Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry, 19, 1083–1084.

    Article  CAS  Google Scholar 

  18. Li, L., Abe, Y., Kanagawa, K., Usui, N., Imai, K., Mashino, T., et al. (2004). Analytica Chimica Acta, 512, 121–124.

    Article  CAS  Google Scholar 

  19. Rosenkranz, A. R., Schmaldienst, S., Stuhlmeier, K. M., Chen, W., Knapp, W., & Zlabinger, G. J. (1992). Journal of Immunological Methods, 156, 39–45.

    Article  CAS  Google Scholar 

  20. Singh, N. P. (2000). Mutation Research: Fundamental and Molecular Mechanisms of Mutagenesis, 455, 111–127.

    Article  CAS  Google Scholar 

  21. Rajagopalan, R., Ranjan, S. K., & Nair, C. K. (2003). Mutation Research, 536, 15–25.

    Article  CAS  Google Scholar 

  22. Troiano, L., Ferraresi, R., Lugli, E., Nemes, E., Roat, E., Nasi, M., et al. (2007). Nature Protocols, 2, 2719–2727.

    Article  CAS  Google Scholar 

  23. Yuan, J., Adamski, R., & Chen, J. (2010). FEBS Letters, 584, 3717–3724.

    Article  CAS  Google Scholar 

  24. Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., & Bonner, W. M. (1998). The Journal of Biological Chemistry, 273, 5858–5868.

    Article  CAS  Google Scholar 

  25. Burma, S., Chen, B. P., Murphy, M., Kurimasa, A., & Chen, D. J. (2001). The Journal of Biological Chemistry, 276, 42462–42467.

    Article  CAS  Google Scholar 

  26. Rogakou, E. P., Boon, C., Redon, C., & Bonner, W. M. (1999). The Journal of Cell Biology, 146, 905–916.

    Article  CAS  Google Scholar 

  27. Kulms, D., Zeise, E., Poppelmann, B., & Schwarz, T. (2002). Oncogene, 21, 5844–5851.

    Article  CAS  Google Scholar 

  28. Liebel, F., Kaur, S., Ruvolo, E., Kollias, N., & Southall, M. D. (2012). The Journal of Investigative Dermatology, 132, 1901–1907.

    Article  CAS  Google Scholar 

  29. De Gruijl, F. R., Sterenborg, H. J., Forbes, P. D., Davies, R. E., Cole, C., Kelfkens, G., et al. (1993). Cancer Research, 53, 53–60.

    Google Scholar 

  30. Fisher, G. J., Wang, Z. Q., Datta, S. C., Varani, J., Kang, S., & Voorhees, J. J. (1997). The New England Journal of Medicine, 337, 1419–1428.

    Article  CAS  Google Scholar 

  31. Ryoo, I., Moon, E. Y., Kim, Y. H., Lee, I., Choo, S. J., Bae, K. H., et al. (2010). Biomolecules & Therapeutics, 18, 300–307.

    Article  CAS  Google Scholar 

  32. Tseng, T. H., Wang, C. J., Kao, E. S., & Chu, H. Y. (1996). Chemico-Biological Interactions, 101, 137–148.

    Article  CAS  Google Scholar 

  33. Sroka, Z., & Cisowski, W. (2003). Food and Chemical Toxicology, 41, 753–758.

    Article  CAS  Google Scholar 

  34. Tanaka, T., Kojima, T., Kawamori, T., Yoshimi, N., & Mori, H. (1993). Cancer Research, 53, 2775–2779.

    CAS  Google Scholar 

  35. Kawamori, T., Tanaka, T., Kojima, T., Suzui, M., Ohnishi, M., & Mori, H. (1994). Japanese Journal of Cancer Research, 85, 686–691.

    Article  CAS  Google Scholar 

  36. Tanaka, T., Kojima, T., Kawamori, T., & Mori, H. (1995). Cancer, 75, 1433–1439.

    Article  CAS  Google Scholar 

  37. Nakamura, H., Nishikawa, A., Furukawa, F., Kasahara, K., Miyauchi, M., Son, H. Y., et al. (2000). Anticancer Research, 20, 3423–3427.

    CAS  Google Scholar 

  38. Babich, H., Sedletcaia, A., & Kenigsberg, B. (2002). Pharmacology & Toxicology, 91, 245–253.

    Article  CAS  Google Scholar 

  39. Lin, H. H., Chen, J. H., Huang, C. C., & Wang, C. J. (2007). Journal International du Cancer, 120, 2306–2316.

    Article  CAS  Google Scholar 

  40. Yasui, H., & Sakurai, H. (2000). Biochemical and Biophysical Research Communications, 269, 131–136.

    Article  CAS  Google Scholar 

  41. Fang, Y., Hu, X. H., Jia, Z. G., Xu, M. H., Guo, Z. Y., & Gao, F. H. (2012). The Australasian Journal of Dermatology, 53, 172–180.

    Article  Google Scholar 

  42. Vostalova, J., Zdarilova, A., & Svobodova, A. (2010). Archives of Dermatological Research, 302, 171–181.

    Article  Google Scholar 

  43. Paz, M. L., Gonzalez Maglio, D. H., Weill, F. S., Bustamante, J., & Leoni, J. (2008). Photodermatology Photoimmunology and Photomedicine, 24, 115–122.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (Korean Ministry of Education, Science, and Technology (MEST)) (NRF-C1ABA001-2011-0021037).

Author information

Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, Republic of Korea

    Ji Won Cha, Mei Jing Piao, Ki Cheon Kim, Jian Zheng, Cheng Wen Yao, Chang Lim Hyun, Hee Kyoung Kang, Eun Sook Yoo, Young Sang Koh & Jin Won Hyun

  2. Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    Nam Ho Lee

  3. Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Republic of Korea

    Mi Hee Ko

Authors
  1. Ji Won Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mei Jing Piao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ki Cheon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cheng Wen Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chang Lim Hyun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hee Kyoung Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Eun Sook Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Young Sang Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Nam Ho Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Mi Hee Ko
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Jin Won Hyun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin Won Hyun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cha, J.W., Piao, M.J., Kim, K.C. et al. Protective Effect of 3,4-Dihydroxybenzoic Acid Isolated from Cladophora wrightiana Harvey Against Ultraviolet B Radiation-Induced Cell Damage in Human HaCaT Keratinocytes. Appl Biochem Biotechnol 172, 2582–2592 (2014). https://doi.org/10.1007/s12010-013-0711-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0711-3

Keywords

Search

Navigation