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Superparamagnetic Polymer Emulsion Particles from a Soap-Free Seeded Emulsion Polymerization and their Application for Lipase Immobilization

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Abstract

Using emulsion copolymer of styrene (St), glycidyl methacrylate (GMA) and 2-hydroxyethyl methacrylate (HEMA) as seed latexes, the superparamagnetic polymer emulsion particles were prepared by seeded emulsion copolymerization of butyl methacrylate (BMA), vinyl acetate (VAc) and ethylene glycol dimethacrylate in the presence of the seed latexes and superparamagnetic Fe3O4/SiOx nanoparticles (or Fe3O4-APTS nanoparticles) through a two-step process, without addition of any emulsifier. The magnetic emulsion particles named P(St-GMA-HEMA)/P(BMA-VAc) were characterized by transmission electron microscope and vibrating sample magnetometry. The results showed that the magnetic emulsion particles held a structure with a thinner shell (around 100 nm) and a bigger cavity (around 200 nm), and possessed a certain level of magnetic response. The resulting magnetic emulsion particles were employed in the immobilization of lipase by two strategies to immobilized lipase onto the resulting magnetic composites directly (S-1) or using glutaraldehyde as a coupling agent (S-2), thus, experimental data showed that the thermal stability and reusability of immobilized lipase based on S-2 were higher than that of S-1.

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Acknowledgments

The authors thank the financial supports from the National Natural Science Foundation of China (No.21374045, No.21074049), the National Natural Science Foundation for the scientific research ability training of undergraduate students majoring in chemistry by the two patterns based on the tutorial system and top students (J1103307) and the Opening Foundation of State Key Laboratory of Applied Organic Chemistry (SKLAOC-2009-35).

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Authors and Affiliations

  1. State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China

    Yanjun Cui, Xia Chen, Yanfeng Li, Xiao Liu, Lin Lei, Yakui Zhang & Jiayu Qian

  2. Lanzhou Petrochemical Research Center of PetroChina, Lanzhou, 730060, China

    Yanjun Cui

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  1. Yanjun Cui
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  2. Xia Chen
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  3. Yanfeng Li
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  4. Xiao Liu
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  7. Jiayu Qian
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Correspondence to Yanfeng Li.

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Cui, Y., Chen, X., Li, Y. et al. Superparamagnetic Polymer Emulsion Particles from a Soap-Free Seeded Emulsion Polymerization and their Application for Lipase Immobilization. Appl Biochem Biotechnol 172, 701–712 (2014). https://doi.org/10.1007/s12010-013-0563-x

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  • DOI: https://doi.org/10.1007/s12010-013-0563-x

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