Skip to main content
SpringerLink
Log in

Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

ρ-Benzoquinone-activated alginate beads were presented as a new carrier for affinity covalent immobilization of glucoamylase enzyme. Evidences of alginate modification were extracted from FT-IR and thermal gravimetric analysis and supported by morphological changes recognized through SEM examination. Factors affecting the modification process such as ρ-benzoquinone (PBQ) concentration, reaction time, reaction temperature, reaction pH and finally alginate concentration, have been studied. Its influence on the amount of coupled PBQ was consequently correlated to the changes of the catalytic activity and the retained activity of immobilized enzyme, the main parameters judging the success of the immobilization process. The immobilized glucoamylase was found kept almost 80% of its native activity giving proof of non-significant substrate, starch, diffusion limitation. The proposed affinity covalent immobilizing technique would rank among the potential strategies for efficient immobilization of glucoamylase enzyme.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Loska, J., Wlodarczyk, W., & Zaborska, J. (1999). Journal of Molecular Catalysis. B, Enzymatic, 6, 549–553.

    Article  Google Scholar 

  2. Arica, M. Y., & Alaeddinoglu, N. G. V. (1998). Enzyme and Microbial Technology, 22, 152–157.

    Article  CAS  Google Scholar 

  3. Arica, M. Y., Handan, Y., Patir, S., & Denizli, A. (2000). Journal of Molecular Catalysis. B, Enzymatic, 11, 127–138.

    Article  CAS  Google Scholar 

  4. Arica, M. Y., Hasirci, V., & Alaeddinogˇlu, N. G. (1995). Biomaterials, 16, 761.

    Article  CAS  Google Scholar 

  5. Aksoy, S., Tümtürk, H., & Hasirci, N. (1998). Journal of Biotechnology, 60, 37.

    Article  CAS  Google Scholar 

  6. Crabb, W. D., & Mitchinson, C. (1997). Trends in Biotechnology, 15, 349–352.

    Article  CAS  Google Scholar 

  7. Silva, R. N., Asquieri, E. R., & Fernandes, K. F. (2005). Process Biochemistry, 40, 1155–1159.

    Article  CAS  Google Scholar 

  8. Tanriseven, A., Bozkurt Uluda, Y., & Dogan, S. (2002). Enzyme and Microbial Technology, 30, 406–409.

    Article  CAS  Google Scholar 

  9. Bahar, T., & Celebi, S. S. (1998). Enzyme and Microbial Technology, 23, 301–304.

    Article  CAS  Google Scholar 

  10. Yajima, H., Hirose, A., Ishii, T., Ohsawa, T., & Endo, R. (1989). Biotechnology and Bioengineering, 33, 795–798.

    Article  CAS  Google Scholar 

  11. Oh, J. T., & Kim, J. H. (2000). Enzyme and Microbial Technology, 27, 356–366.

    Article  CAS  Google Scholar 

  12. Tanaka, A. (1996). Bioscience, Biotechnology, and Biochemistry, 60, 2055–2058.

    Article  CAS  Google Scholar 

  13. Tanaka, H., Kurosawa, H., Kokufuta, E., & Veliky, I. A. (1984). Biotechnology and Bioengineering, 26, 1393–1399.

    Article  CAS  Google Scholar 

  14. Katchalski-Katzir, E. (1993). Trends in Biotechnology, 11, 471–478.

    Article  CAS  Google Scholar 

  15. Chibata, I., Tosa, T., & Sato, T. (1986). Journal of Molecular Catalysis. B, Enzymatic, 37, 1–24.

    CAS  Google Scholar 

  16. Hong, J., Xu, D., Gong, P., Yu, J., Ma, H., & Yao, S. (2008). Microporous and Mesoporous Materials, 109, 470–477.

    Article  CAS  Google Scholar 

  17. Li, Z. F., Kang, E. T., Neoh, K. G., & Tan, K. L. (1998). Biomaterials, 19, 45–53.

    Article  CAS  Google Scholar 

  18. Liu, C., Kuwahara, T., Yamazaki, R., & Shimomura, M. (2000). European Polymer Journal, 36, 2095–2103.

    Article  Google Scholar 

  19. Ying, L., Kang, E. T., & Neoh, K. G. (2002). Journal of Membrane Science, 208, 361–374.

    Article  CAS  Google Scholar 

  20. Liu, C., Kuwahara, T., Yamazaki, R., & Shimomura, M. (2007). European Polymer Journal, 43, 3264–3276.

    Article  CAS  Google Scholar 

  21. Mohy Eldin, M. S. (2005). Deutsch lebensmittel-Rundschau, 101, 193–198.

    CAS  Google Scholar 

  22. Mohy Eldin, M. S., Hassan, E. A., & Elaassar, M. R. (2005). Deutsch lebensmittel-Rundschau, 101, 255–259.

    CAS  Google Scholar 

  23. Mohy Eldin, M. S. (2005). Deutsch lebensmittel-Rundschau, 101, 309–314.

    CAS  Google Scholar 

  24. Schroën, C. G. P. H., Mohy Eldin, M. S., Janssen, A. E. M., Mita, G. D., & Tramper, J. (2001). Journal of Molecular Catalysis. B, Enzymatic, 15, 163–172.

    Article  Google Scholar 

  25. Mohy Eldin, M. S., Bencivenga, U., Rossi, S., Canciglia, P., Gaeta, F. S., Tramper, J., et al. (2000). Journal of Molecular Catalysis. B, Enzymatic, 8, 233–244.

    Article  CAS  Google Scholar 

  26. Rosell, G. M., Fernandez-Lafuente, R., & Jusian, J. M. (1995). Biocatalysis and Biotransformation, 12, 67–76.

    Article  CAS  Google Scholar 

  27. Blanco, R. M., & Jusian, J. M. (1989). Enzyme and Microbial Technology, 11, 360–366.

    Article  CAS  Google Scholar 

  28. Sardar, M., & Gupta, M. N. (1998). Bioseparation, 7, 159–165.

    Article  CAS  Google Scholar 

  29. Sharma, A., Sharma, S., & Gupta, M. N. (2000). Protein Expression and Purification, 18, 111–114.

    Article  CAS  Google Scholar 

  30. Roy, I., Sastry, M. S. R., Johri, B. N., & Gupta, M. N. (2000). Protein Expression and Purification, 20, 162–168.

    Article  CAS  Google Scholar 

  31. Lee, P. M., Lee, K. H., & Siaw, Y. S. (1993). Journal of Chemical Technology and Biotechnology, 58, 65–70.

    CAS  Google Scholar 

  32. Li, T., Wang, N., Li, S., Zhao, Q., & Guo, M. (2007). Biotechnological Letters, 29, 1411–1416.

    Google Scholar 

  33. Teotia, S., Lata, R., Khare, S. K., & Gupta, M. N. (2001). Journal of Molecular Recognition, 14, 295–299.

    Article  CAS  Google Scholar 

  34. Serour, E., & Antranikian, G. (2002). Antonie van Leeuwenhoek, 81, 73–83.

    Article  CAS  Google Scholar 

  35. Sanjay, G., & Sugunan, S. (2005). Cataly Comuna, 6, 525–530.

    Article  CAS  Google Scholar 

  36. deOlive iraa, A. N., de Santanaa, H., Zaiab, C. T. B. V., & Zaiaa, D. A. M. (2004). Journal of Food Composition and Analysis, 17, 165–177.

    Article  Google Scholar 

  37. Kalman, M., Szajani, B., & Boross, L. (1983). Applied Biochemistry and Biotechnology, 8, 515–522.

    Article  CAS  Google Scholar 

  38. Chun, M., Dickopp, G., & Sernetz, M. (1980). Journal of Solid-Phase Biochemistry, 5, 211–221.

    Article  CAS  Google Scholar 

  39. Rebroˇs, M., Rosenberg, M., Mlichová, Z., Krištofíkovš, L., & Paluch, M. (2006). Enzyme and Microbial Technology, 39, 800–804.

    Article  Google Scholar 

  40. Dhar, G. M., Mitsutomi, M., & Ohtakara, A. (1993). Bulletin of the Faculty of Agriculture, Saga University, 74, 59–68.

    CAS  Google Scholar 

  41. Mateescu, M., Weltrowska, G., Agostinelli, E., Saint-Andre, R., Weltrowski, M., & Mondovi, B. (1989). Biotechnology Techniques, 3, 415–420.

    Article  CAS  Google Scholar 

  42. Sanjay, G., & Sugunan, S. (2005). Catalysis Communications, 6, 525–530.

    Article  CAS  Google Scholar 

  43. Park, D., Haam, S., Jang, K., Ahn, Ik, & Kim, W. (2005). Process Biochemistry, 40, 53–61.

    Article  CAS  Google Scholar 

  44. Mohy Eldin, M. S., Soliman, E. A., Hashem, A. I., & Tamer, T. M. (2008). Trends in Biomaterials and Artificial Organs, 22, 154–164.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Polymer Materials Research Department, Advanced Technologies and New Material Research Institute, Mubarak City for Scientific Research and Technology Applications, New Borg El-Arab City, 21934, Alexandria, Egypt

    M. S. Mohy Eldin, M. R. El-Aassar & H. A. Tieama

  2. Department of Chemistry, Faculty of Science, Alexandria University, Alexandria, Egypt

    M. A. Nasr

  3. Protein Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, New Borg El-Arab City, 21934, Alexandria, Egypt

    E. I. Seuror

Authors
  1. M. S. Mohy Eldin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. I. Seuror
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. A. Nasr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. R. El-Aassar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. A. Tieama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. S. Mohy Eldin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mohy Eldin, M.S., Seuror, E.I., Nasr, M.A. et al. Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization. Appl Biochem Biotechnol 164, 10–22 (2011). https://doi.org/10.1007/s12010-010-9110-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-010-9110-1

Keywords

Search

Navigation