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Effect and Modeling of Glucose Inhibition and In Situ Glucose Removal During Enzymatic Hydrolysis of Pretreated Wheat Straw

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Abstract

The enzymatic hydrolysis of lignocellulosic biomass is known to be product-inhibited by glucose. In this study, the effects on cellulolytic glucose yields of glucose inhibition and in situ glucose removal were examined and modeled during extended treatment of heat-pretreated wheat straw with the cellulolytic enzyme system, Celluclast® 1.5 L, from Trichoderma reesei, supplemented with a β-glucosidase, Novozym® 188, from Aspergillus niger. Addition of glucose (0–40 g/L) significantly decreased the enzyme-catalyzed glucose formation rates and final glucose yields, in a dose-dependent manner, during 96 h of reaction. When glucose was removed by dialysis during the enzymatic hydrolysis, the cellulose conversion rates and glucose yields increased. In fact, with dialytic in situ glucose removal, the rate of enzyme-catalyzed glucose release during 48–72 h of reaction recovered from 20–40% to become ≈70% of the rate recorded during 6–24 h of reaction. Although Michaelis–Menten kinetics do not suffice to model the kinetics of the complex multi-enzymatic degradation of cellulose, the data for the glucose inhibition were surprisingly well described by simple Michaelis–Menten inhibition models without great significance of the inhibition mechanism. Moreover, the experimental in situ removal of glucose could be simulated by a Michaelis–Menten inhibition model. The data provide an important base for design of novel reactors and operating regimes which include continuous product removal during enzymatic hydrolysis of lignocellulose.

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Acknowledgment

The authors thank Novozymes A/S for providing the enzymes. Dong Energy A/S is acknowledged for supplying the pretreated wheat straw. Dr. Gürkan Sin, Department of Chemical and Biochemical Engineering, Technical University of Denmark, is acknowledged for his help with the numerical simulations. This work is part of the CHEC (Combustion and Harmful Emission Control) Research Center funded a.o. by the Technical University of Denmark, the Danish Technical Research Council, the European Union, the Nordic Energy Research, Dong Energy A/S, Vattenfall A.B., F L Smidth A/S, and Public Service Obligation funds from Energinet.dk and the Danish Energy Research program.

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  1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs, Lyngby, Denmark

    Pavle Andrić, Anne S. Meyer, Peter A. Jensen & Kim Dam-Johansen

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  1. Pavle Andrić
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  2. Anne S. Meyer
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  4. Kim Dam-Johansen
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Correspondence to Anne S. Meyer.

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Andrić, P., Meyer, A.S., Jensen, P.A. et al. Effect and Modeling of Glucose Inhibition and In Situ Glucose Removal During Enzymatic Hydrolysis of Pretreated Wheat Straw. Appl Biochem Biotechnol 160, 280–297 (2010). https://doi.org/10.1007/s12010-008-8512-9

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