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Spermine-Induced Morphogenesis and Effect of Partial Immersion System on the Shoot Cultures of Banana

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Abstract

Contribution of exogenous polyamines (PAs) and polyamine-inhibitors on plantlet regeneration patterns of banana (cv. Nanjanagudu Rasabale-AAB) was studied and the performance of regenerated shoots in temporary immersion system was evaluated. The rhizome explants (without shoot bud) of in vitro shoots produced a mixture of embryogenic and nonembryogenic calli on modified MS medium. The analyses of endogenous pools of polyamines showed higher levels of PAs in embryogenic than in nonembryogenic calli. Supplementation of various levels of (10–50 μM) spermine (Spm), spermidine (Spd), and putrescine (Put) to cultures with secondary embryogenesis showed that about 50% of embryogenic calli rapidly produced secondary embryos only in the presence 40 μM Spm but not in other treatments. The crucial role of Spm was further confirmed by the use of 0.1 mM each of α-DL-Difluromethylornithine and α-DL-Difluromethylarginine along with Spm where the presence of inhibitors concomitantly inhibited the secondary embryogenesis. The shoots obtained from the embryogenic cultures were checked for their performance on solid medium (SM) and partial immersion system (PIS). The rate of shoot multiplication was higher in PIS than in SM throughout 6 weeks culture period. Uniformity in elongation of all the shoot buds was observed in PIS but not in SM. Evaluation for the acclimatization, survival under greenhouse conditions revealed the better performance of PIS-derived plants than those from SM.

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Abbreviations

2iP:

N6-(2-isopentenyl)adenine

BA:

Benzyladenine

DFMA:

α-DL-Difluromethylarginine

DFMO:

α-DL-Difluromethylornithine

GA:

Gibberellic acid

HPLC:

High performance liquid chromatography

PAs:

Polyamines

PIS:

Partial Immersion System

Put:

Putrescine

SM:

Solid Medium

Spd:

Spermidine

Spm:

Spermine

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Acknowledgment

First author acknowledges Council of Scientific and Industrial Research, India, for the financial support in the form of Research Fellowship. Encouragement by Dr. V. Prakash, Director, CFTRI and Dr. G.A. Ravishankar, Head, PCBT, CFTRI is also gratefully acknowledged.

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  1. Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore, 570 020, India

    L. Venkatachalam & N. Bhagyalakshmi

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  1. L. Venkatachalam
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  2. N. Bhagyalakshmi
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Correspondence to N. Bhagyalakshmi.

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Venkatachalam, L., Bhagyalakshmi, N. Spermine-Induced Morphogenesis and Effect of Partial Immersion System on the Shoot Cultures of Banana. Appl Biochem Biotechnol 151, 502–511 (2008). https://doi.org/10.1007/s12010-008-8226-z

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  • DOI: https://doi.org/10.1007/s12010-008-8226-z

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