Abstract
Poly (2-methacryloyloxyethyl phosphorylcholine) (PMPC) has been widely used for surface modification of titanium (Ti) alloys to improve antifouling properties and blood compatibility. To achieve antifouling and anticoagulant capacities via a facile and efficient technique, PMPC coatings were covalently immobilized on a Ti surface simply by a sequential dip-coating method. The Ti surface was first dipped into hydrochloric acid (HCl) solution to remove surface contaminants and acquire a hydroxyl-enriched surface. Then, the surface was dipped into dopamine solution to acquire the surface bearing –NH2 groups. N-acryloxysuccinimide solution was used for introduction of double linkage which could graft with PMPC via in situ free-radical polymerization. Thus, the surface modification processes were under mild conditions and easy scale up. The obtained PMPC coatings endowed the Ti surface with smooth finish and relatively high packing density. Water contact angle measurements showed that the hydrophilicity of the Ti surface was markedly improved by coating PMPC with the water contact angle of 6.2°. Furthermore, the PMPC coatings could effectively resist protein adsorption and suppress platelet adhesion and activation. The facile and efficient method of immobilization of PMPC on a Ti surface could be promising for a variety of cardiovascular applications.
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Ye, SH, Johnson, CA, Jr, Woolley, JR, Murata, H, Gamble, LJ, Ishihara, K, Wagner, WR, “Simple Surface Modification of a Titanium Alloy with Silanated Zwitterionic Phosphorylcholine or Sulfobetaine Modifiers to Reduce Thrombogenicity.” Colloids Surf., B: Biointerfaces, 79 (2) 357–364 (2010)
Li, J, Zhao, Y, “Biocompatibility and Antibacterial Performance of Titanium by Surface Treatment.” J. Coat. Technol. Res., 9 (2) 223–228 (2012)
Pan, CJ, Hou, YH, Ding, HY, Dong, YX, “Enhancing Anticoagulation and Endothelial Cell Proliferation of Titanium Surface by Sequential Immobilization Of Poly (Ethylene Glycol) and Collagen.” Appl. Surf. Sci., 287 443–450 (2013)
Bakir, M, “Haemocompatibility of Titanium and Its Alloys.” J. Biomater. Appl., 27 (1) 3–15 (2012)
Pan, CJ, Hou, YH, Liu, HQ, Ding, HY, Dong, YX, “Improved Anticoagulation of Titanium by Sequential Immobilization of Oligo (Ethylene Glycol) and 2-Methacryloyloxyethyl Phosphorylcholine.” Colloids Surf., B: Biointerfaces, 112 508–512 (2013)
Vadillo-Rodríguez, V, Pacha-Olivenza, MA, Gónzalez-Martín, ML, Bruque, JM, Gallardo-Moreno, AM, “Adsorption Behavior of Human Plasma Fibronectin on Hydrophobic and Hydrophilic Ti6Al4V Substrata and Its Influence on Bacterial Adhesion and Detachment.” J. Biomed. Mater. Res., 101 (5) 1397–1404 (2013)
Yu, BY, Zheng, J, Chang, Y, Sin, MC, Chang, CH, Higuchi, A, Sun, YM, “Surface Zwitterionization of Titanium for a General Bio-Inert Control of Plasma Proteins, Blood Cells, Tissue Cells, and Bacteria.” Langmuir, 30 (25) 7502–7512 (2014)
Sin, DC, Kei, HL, Miao, X, “Surface Coatings for Ventricular Assist Devices.” Expert Rev. Med. Devices, 6 (1) 51–60 (2009)
Mitamura, Y, Hosooka, K, Matsumoto, T, Otaki, K, Sakai, K, Tanabe, T, Yuta, T, Mikami, T, “Development of a Ceramic Heart Valve.” J. Biomater. Appl., 4 (1) 33–55 (1989)
Hauert, R, “A Review of Modified DLC Coatings for Biological Applications.” Diam Rel. Mater., 12 (3) 583–589 (2003)
Ma, W, Yang, P, Li, J, Li, S, Li, P, Zhao, Y, Huang, N, “Immobilization of Poly (MPC) Brushes Onto Titanium Surface by Combining Dopamine Self-Polymerization and ATRP: Preparation, Characterization and Evaluation of Hemocompatibility in Vitro.” Appl. Surf. Sci., 349 445–451 (2015)
Pan, CJ, Hou, YH, Zhang, BB, Dong, YX, Ding, HY, “Blood Compatibility and Interaction with Endothelial Cells of Titanium Modified by Sequential Immobilization Of Poly (Ethylene Glycol) and Heparin.” J. Mater. Chem. B, 2 (7) 892–902 (2014)
Zhou, L, Tan, GX, Ning, CY, “Modification of Biomaterials Surface by Mimetic Cell Membrane to Improve Biocompatibility.” Front. Mater. Sci., 8 (4) 325–331 (2014)
Gao, B, Konno, T, Ishihara, K, “Cytocompatible and Spontaneously Forming Phospholipid Polymer Hydrogels.” Eur. Polym. J., 72 577–589 (2015)
Gong, M, Wang, YB, Li, M, Hu, BH, Gong, YK, “Fabrication and Hemocompatibility of Cell Outer Membrane Mimetic Surfaces on Chitosan by Layer by Layer Assembly with Polyanion Bearing Phosphorylcholine Groups.” Colloids Surf., B: Biointerfaces, 85 (1) 48–55 (2011)
Dang, Y, Quan, M, Xing, CM, Wang, YB, Gong, YK, “Biocompatible and Antifouling Coating of Cell Membrane Phosphorylcholine and Mussel Catechol Modified Multi-Arm PEGs.” J. Mater. Chem. B, 3 (11) 2350–2361 (2015)
Zhou, T, Zhu, Y, Li, X, Liu, X, Yeung, KWK, Wu, S, Wang, X, Cui, Z, Yang, X, Chu, PK, “Surface Functionalization of Biomaterials by Radical Polymerization.” Prog. Mater. Sci., 83 191–235 (2016)
Yao, Y, Fukazawa, K, Ma, W, Ishihara, K, Huang, N, “Platelet Adhesion-Resistance of Titanium Substrate with Mussel-Inspired Adhesive Polymer Bearing Phosphorylcholine Group.” Appl. Surf. Sci., 258 (14) 5418–5423 (2012)
Yu, J, Wei, W, Menyo, MS, Masic, A, Waite, JH, Israelachvili, JN, “Adhesion of Mussel Foot Protein-3 to TiO2 Surfaces: The Effect of pH.” Biomacromolecules, 14 (4) 1072–1077 (2013)
Xie, Y, Wang, R, Li, S, Xiang, T, Zhao, CS, “A Robust Way to Prepare Blood-Compatible and Anti-Fouling Polyethersulfone Membrane.” Colloids Surf., B: Biointerfaces, 146 326–333 (2016)
Cheng, C, Khoshdel, E, Wooley, KL, “Facile One-Pot Synthesis of Brush Polymers Through Tandem Catalysis Using Grubbs’ Catalyst for Both Ring-Opening Metathesis and Atom Transfer Radical Polymerizations.” Nano Lett., 6 (8) 1741–1746 (2006)
Vogt, AP, Sumerlin, BS, “Temperature and Redox Responsive Hydrogels from ABA Triblock Copolymers Prepared by RAFT Polymerization.” Soft Matter, 5 (12) 2347–2351 (2009)
Matyjaszewski, K, Tsarevsky, NV, “Macromolecular Engineering by Atom Transfer Radical Polymerization.” J. Am. Chem. Soc., 136 (18) 6513–6533 (2014)
Kakuchi, R, “Multicomponent Reactions in Polymer Synthesis.” Angew. Chem. Int. Ed., 53 (1) 46–48 (2014)
Qin, H, Sun, C, He, C, Wang, D, Cheng, C, Nie, S, Sun, S, Zhao, C, “High Efficient Protocol for the Modification of Polyethersulfone Membranes with Anticoagulant and Antifouling Properties Via in Situ Cross-Linked Copolymerization.” J. Membr. Sci., 468 172–183 (2014)
Waite, JH, “Surface Chemistry: Mussel Power.” Nat. Mater., 7 (1) 8–9 (2008)
Heo, S, Jeon, YS, Kim, YJ, Kim, SH, Kim, JH, “Bioinspired Self-Adhesive Polymer for Surface Modification to Improve Antifouling Property.” J. Coat. Technol. Res., 10 (6) 811–819 (2013)
Takeuchi, M, Abe, Y, Yoshida, Y, Nakayama, Y, Okazaki, M, Akagawa, Y, “Acid Pretreatment of Titanium Implants.” Biomaterials, 24 (10) 1821–1827 (2003)
Nebe, JB, Müller, L, Lüthen, F, Ewald, A, Bergemann, C, Conforto, E, Müller, FA, “Osteoblast Response to Biomimetically Altered Titanium Surfaces.” Acta Biomater., 4 (6) 1985–1995 (2008)
Sun, M, Su, Y, Mu, C, Jiang, Z, “Improved Antifouling Property of PES Ultrafiltration Membranes Using Additive of Silica–PVP Nanocomposite.” Ind. Eng. Chem. Res., 49 (2) 790–796 (2009)
Yoshimoto, K, Hirase, T, Madsen, J, Armes, SP, Nagasaki, Y, “Non-Fouling Character of Poly [2-(methacryloyloxy) Ethyl Phosphorylcholine]-Modified Gold Surfaces Fabricated by the ‘Grafting to’ Method: Comparison of Its Protein Resistance with Poly (Ethylene Glycol)-Modified Gold Surfaces.” Macromol. Rapid Commun., 30 (24) 2136–2140 (2009)
Feng, W, Zhu, S, Ishihara, K, Brash, JL, “Protein Resistant Surfaces: Comparison of Acrylate Graft Polymers Bearing Oligo-Ethylene Oxide and Phosphorylcholine Side Chains.” Biointerphases, 1 (1) 50–60 (2006)
Agnihotri, A, Soman, P, Siedlecki, CA, “AFM Measurements of Interactions Between the Platelet Integrin Receptor GPIIbIIIa and Fibrinogen.” Colloids Surf., B: Biointerfaces, 71 (1) 138–147 (2009)
Yao, Y, Fukazawa, K, Huang, N, Ishihara, K, “Bioinspired Phospholipid Polymer for Improvement of Biofouling on Titanium Alloy Substrate.” Trans. Mater. Res. Soc. Jpn., 36 (4) 573–576 (2011)
Wei, Q, Krysiak, S, Achazi, K, Becherer, T, Michael Noeske, PL, Paulus, F, Liebe, H, Grunwald, I, Dernedde, J, Hartwig, A, Hugel, T, Haag, R, “Multivalent Anchored and Crosslinked Hyperbranched Polyglycerol Monolayers as Antifouling Coating for Titanium Oxide Surfaces.” Colloids Surf., B: Biointerfaces, 122 684–692 (2014)
Chen, H, Yuan, L, Song, W, Wu, Z, Li, D, “Biocompatible Polymer Materials: Role of Protein–Surface Interactions.” Prog. Polym. Sci., 33 (11) 1059–1087 (2008)
Liu, X, Yuan, L, Li, D, Tang, Z, Wang, Y, Chen, G, Chen, H, Brash, JL, “Blood Compatible Materials: State of the Art.” J. Mater. Chem. B., 2 (35) 5718–5738 (2014)
Acknowledgments
This work was sponsored by the International Science and Technology Cooperation Program of China (Grant No. 2012DFA51690); the National Natural Science Foundation of China (Grant Nos. 31370970, 51303125); and the Tianjin Research Program of Application Foundation and Advanced Technology (Grant Nos. 13JCZDJC33300, 14JCZDJC38500).
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Wu, C., Chang, W., Qi, H. et al. A facile technique for fabricating poly (2-methacryloyloxyethyl phosphorylcholine) coatings on titanium alloys. J Coat Technol Res 14, 1127–1135 (2017). https://doi.org/10.1007/s11998-016-9900-7
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DOI: https://doi.org/10.1007/s11998-016-9900-7