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Shelf Life Assessment of Modified Atmosphere Packaged Turbot (Psetta maxima) Fillets: Evaluation of Microbial, Physical and Chemical Quality Parameters

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Abstract

Quality and safety of turbot fillets under modified atmosphere packaging (MAP) were assessed by microbial (total viable counts), physical (drip loss, pH, colour CIE Lab) and chemical parameters (total volatile basic nitrogen (TVBN), trimethylamine nitrogen (TMAN), biogenic amine contents). Three different atmospheres (MAP 1, 10/40/50 % O2/CO2/N2; MAP 2, 10/60/30 % O2/CO2/N2; MAP 3, 10/80/10 % O2/CO2/N2) were tested. Packaged turbot fillets were stored at 2 ± 1 °C and monitored over 30 days, at intervals of 5 days. Fillets from the control group, packaged with air (AIR), were the first to present signs of degradation reaching rejection threshold values for all evaluated parameters. In MAP fillets, total bacterial count was lower than 106 cfu/g for a longer period. After 10 days of storage, MAP and AIR fillets showed significant differences (p < 0.05) on the values of TVBN, TMAN and biogenic amines. MAP fillets presented a higher drip loss, and fillets in AIR became more yellowish (upper b* values) while those in MAP looked whitish for an increased period (upper L* values). All microbial, chemical and physical traits revealed the protective effect of the different MAP studied, especially those with a higher percentage of CO2. MAP application added, at least, 5 days to shelf life of turbot fillets.

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References

  • Antonacopoulos, N., & Vyncke, W. D. (1989). Determination of volatile basic nitrogen in fish: a third collaborative study by the West European Fish Technologists' Association (WEFTA). Zeitschrift für Lebensmitteluntersuchung und -Forschung A, 189(4), 309–316.

    Article  CAS  Google Scholar 

  • AOAC. (2000). AOAC official method of analysis 971.14. chapter 35.

  • Aubourg, S. P., Piñeiro, C., Gallardo, J. M., & Barros-Velazquez, J. (2005). Biochemical changes and quality loss during chilled storage of farmed turbot (Psetta maxima). Food Chemistry, 90(3), 445–452.

    Article  CAS  Google Scholar 

  • Buckley, M., Cowan, C., & McCarthy, M. (2007). The convenience food market in Great Britain: convenience food lifestyle (CFL) segments. Appetite, 49(3), 600–617.

    Article  Google Scholar 

  • Bulushi, I. A., Poole, S., Deeth, H. C., & Dykes, G. A. (2009). Biogenic amines in fish: roles in intoxication, spoilage, and nitrosamine formation—a review. Critical Reviews in Food Science and Nutrition, 49(4), 369–377.

    Article  Google Scholar 

  • Caleb, O. J. O., Opara, U. L., & Witthuhn, C. R. (2012). Modified atmosphere packaging of pomegranate fruit and arils: a review. Food Bioprocess Technology, 5, 15–30.

    Article  CAS  Google Scholar 

  • Campos, C. A., Losada, V., Rodríguez, Ó., Aubourg, S. P., & Barros-Velázquez, J. (2006). Evaluation of an ozone–slurry ice combined refrigeration system for the storage of farmed turbot (Psetta maxima). Food Chemistry, 97(2), 223–230.

    Article  CAS  Google Scholar 

  • Casal, S., Oliveira, M. B. P. P., & Ferreira, M. A. (2002). Determination of biogenic amines in coffee by an optimized liquid chromatographic method. Jounal of Liquid Chromatography & Related Technology, 25(16), 2535–2549.

    Article  CAS  Google Scholar 

  • Dalgaard, P. (1995). Qualitative and quantitative characterization of spoilage bacteria from packed fish. International Journal of Food Microbiology, 26(3), 319–333.

    Article  CAS  Google Scholar 

  • Dalgaard, P., Madsen, H. L., Samieian, N., & Emborg, J. (2006). Biogenic amine formation and microbial spoilage in chilled garfish (Belone belone belone)—effect of modified atmosphere packaging and previous frozen storage. Journal of Applied Microbiology, 101(1), 80–95.

    Article  CAS  Google Scholar 

  • Debevere, J., & Boskou, G. (1996). Effect of modified atmosphere packaging on the TVB/TMA-producing microflora of cod fillets. International Journal of Food Microbiology, 31(1–3), 221–229.

    Article  CAS  Google Scholar 

  • EC, C. R. (2005). No 2074/2005 of 5 December 2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004. L 338/27 Official Journal of the European Union.

  • Emborg, J., Laursen, B. G., & Dalgaard, P. (2005). Significant histamine formation in tuna (Thunnus albacares) at 2 °C—effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. International Journal of Food Microbiology, 101(3), 263–279.

    Article  CAS  Google Scholar 

  • Esturk, O., Ayhan, Z., & Ustunel, A. I. (2011). Modified atmosphere packaging of “Napoleon” cherry: effect of packaging material and storage time on physical, chemical, and sensory quality. Food Bioprocess Technology. doi:10.1007/s11947-011-0561-3.

  • Garza-Gil, M. D., Varela-Lafuente, M., & Caballero-Miguez, G. (2009). Price and production trends in the marine fish aquaculture in Spain. Aquaculture Research, 40(3), 274–281.

    Article  Google Scholar 

  • Gram, L., & Huss, H. H. (1996). Microbiological spoilage of fish and fish products. International Journal of Food Microbiology, 33(1), 121–137.

    Article  CAS  Google Scholar 

  • Hansen, A. Å., Mørkøre, T., Rudi, K., Olsen, E., & Eie, T. (2007). Quality changes during refrigerated storage of MA-packaged pre-rigor fillets of farmed Atlantic cod (Gadus morhua L.) using traditional MAP, CO2 emitter, and vacuum. Journal of Food Science, 72(9), M423–M430.

    Article  CAS  Google Scholar 

  • ICMSF. (1986). Sampling for microbiological analysis: principles and specific applications. Toronto: University of Toronto Press.

    Google Scholar 

  • ISO 4833:2003 (2003). Microbiology of food and animal feeding stuffs—horizontal method for the enumeration of microorganisms—colony-count technique at 30 degrees C. International Standards Organization,

  • Malle, P., Valle, M., & Bouquelet, S. (1996). Assay of biogenic amines involved in fish decomposition. Journal of AOAC International, 79(1), 43–49.

    CAS  Google Scholar 

  • Mastromatteo, M., Conte, A., & Del Nobile, M. (2010). Combined use of modified atmosphere packaging and natural compounds for food preservation. Food Engineering Reviews, 2(1), 28–38.

    Article  CAS  Google Scholar 

  • Noseda, B., Dewulf, J., Goethals, J., Ragaert, P., Van Bree, I., Pauwels, D., Van Langenhove, H., & Devlieghere, F. (2010). Effect of food matrix and pH on the volatilization of bases (TVB) in packed North Atlantic gray shrimp (Crangon crangon): volatile bases in MAP fishery products. Journal of Agricultural and Food Chemistry, 58(22), 11864–11869.

    Article  CAS  Google Scholar 

  • Nuin, M., Alfaro, B., Cruz, Z., Argarate, N., George, S., Le Marc, Y., Olley, J., & Pin, C. (2008). Modelling spoilage of fresh turbot and evaluation of a time—temperature integrator (TTI) label under fluctuating temperature. International Journal of Food Microbiology, 127(3), 193–199.

    Article  Google Scholar 

  • Olsen, S. O., Scholderer, J., Brunsø, K., & Verbeke, W. (2007). Exploring the relationship between convenience and fish consumption: a cross-cultural study. Appetite, 49(1), 84–91.

    Article  Google Scholar 

  • Önal, A. (2007). A review: current analytical methods for the determination of biogenic amines in foods. Food Chemistry, 103(4), 1475–1486.

    Article  Google Scholar 

  • Özogul, Y., Özogul, F., Kuley, E., Özkutuk, A. S., Gökbulut, C., & Köse, S. (2006). Biochemical, sensory and microbiological attributes of wild turbot (Scophthalmus maximus), from the Black Sea, during chilled storage. Food Chemistry, 99(4), 752–758.

    Article  Google Scholar 

  • Paleologos, E. K., Savvaidis, I. N., & Kontominas, M. G. (2004). Biogenic amines formation and its relation to microbiological and sensory attributes in ice-stored whole, gutted and filleted Mediterranean sea bass (Dicentrarchus labrax). Food Microbiology, 21(5), 549–557.

    Article  CAS  Google Scholar 

  • Pantazi, D., Papavergou, A., Pournis, N., Kontominas, M. G., & Savvaidis, I. N. (2008). Shelf-life of chilled fresh Mediterranean swordfish (Xiphias gladius) stored under various packaging conditions: microbiological, biochemical and sensory attributes. Food Microbiology, 25(1), 136–143.

    Article  CAS  Google Scholar 

  • Piñeiro, C., Bautista, R., Rodríguez, Ó., Losada, V., Barros-Velázquez, J., & Aubourg, S. P. (2005). Quality retention during the chilled distribution of farmed turbot (Psetta maxima): effect of a primary slurry ice treatment. International Journal of Food Science and Technology, 40(8), 817–824.

    Article  Google Scholar 

  • Poli, B. M., Messini, A., Parisi, G., Scappini, F., Vigiani, V., Giorgi, G., & Vincenzini, M. (2006). Sensory, physical, chemical and microbiological changes in European sea bass (Dicentrarchus labrax) fillets packed under modified atmosphere/air or prepared from whole fish stored in ice. International Journal of Food Science and Technology, 41(4), 444–454.

    Article  CAS  Google Scholar 

  • Rodríguez, Ó., Barros-Velázquez, J., Piñeiro, C., Gallardo, J. M., & Aubourg, S. P. (2006). Effects of storage in slurry ice on the microbial, chemical and sensory quality and on the shelf life of farmed turbot (Psetta maxima). Food Chemistry, 95(2), 270–278.

    Article  Google Scholar 

  • Roth, B., Schelvis-Smit, R., Stien, L. H., Foss, A., Nortvedt, R., & Imsland, A. (2007). Exsanguination of turbot and the effect on fillet quality measured mechanically, by sensory evaluation, and with computer vision. Journal of Food Science, 72(9), E525–E531.

    Article  CAS  Google Scholar 

  • Roth, B., Foss, A., & Imsland, A. K. (2009). Relationship between muscle pH and flesh color of Atlantic halibut. Journal of Food Science, 74(3), S123–S125.

    Article  CAS  Google Scholar 

  • Ruff, N., Fitzgerald, R. D., Cross, T. F., & Kerry, J. P. (2002). Comparative composition and shelf-life of fillets of wild and cultured turbot (Scophthalmus maximus) and Atlantic halibut (Hippoglossus hippoglossus). Aquaculture International, 10(3), 241–256.

    Article  CAS  Google Scholar 

  • Sivertsvik, M., Jeksrud, W. K., & Rosnes, J. T. (2002). A review of modified atmosphere packaging of fish and fishery products—significance of microbial growth, activities and safety. International Journal of Food Science and Technology, 37(2), 107–127.

    Article  CAS  Google Scholar 

  • Sivertsvik, M., Rosnes, J. T., & Kleiberg, G. H. (2003). Effect of modified atmosphere packaging and superchilled storage on the microbial and sensory quality of Atlantic salmon (Salmo salar) fillets. Journal of Food Science, 68(4), 1467–1472.

    Article  CAS  Google Scholar 

  • Sivertsvik, M., Rosnes, J. T., & Jeksrud, W. K. (2004). Solubility and absorption rate of carbon dioxide into non-respiring foods. Part 2: raw fish fillets. Journal of Food Engineering, 63(4), 451–458.

    Article  Google Scholar 

  • Wang, T., Sveinsdóttir, K., Magnússon, H., & Martinsdóttir, E. (2008). Combined application of modified atmosphere packaging and superchilled storage to extend the shelf life of fresh cod (Gadus morhua) loins. Journal of Food Science, 73(1), S11–S19.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank Instituto Politécnico de Viana do Castelo for providing the gases and the packaging equipment. Joana Santos and Francisca Lisboa are thankful to Fundação para a Ciência e Tecnologia for the PhD grants SFRH/BD/66476/2009 and SFRH/BDE/51385/2011 financed by POPH-QREN and subsidised by ESF and MCTES. This work had been supported by Fundação para a Ciência e Tecnologia through project no. PEst-C/EQB/LA0006/2011.

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Authors and Affiliations

  1. REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal

    Joana Santos, Francisca Lisboa, Nazaré Pestana, Susana Casal & M. Beatriz P. P. Oliveira

  2. REQUIMTE, ESTG, Instituto Politécnico de Viana do Castelo, Av. do Atlântico, Apartado 574, 4900-348, Viana do Castelo, Portugal

    M. Rui Alves

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  1. Joana Santos
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  2. Francisca Lisboa
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  3. Nazaré Pestana
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  4. Susana Casal
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  5. M. Rui Alves
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  6. M. Beatriz P. P. Oliveira
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Correspondence to Joana Santos.

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Santos, J., Lisboa, F., Pestana, N. et al. Shelf Life Assessment of Modified Atmosphere Packaged Turbot (Psetta maxima) Fillets: Evaluation of Microbial, Physical and Chemical Quality Parameters. Food Bioprocess Technol 6, 2630–2639 (2013). https://doi.org/10.1007/s11947-012-0900-z

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  • DOI: https://doi.org/10.1007/s11947-012-0900-z

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