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Competitive activity as a constitutive promoter in the 5′-proximal regulatory region of the Capsicum capsanthin–capsorubin synthase gene

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Abstract

Depending on the nature of the Capsicum capsanthin–capsorubin synthase gene (CaCcs) showing a chromoplast specificity, the 5′-upstream region of CaCcs from −2279 to +30 was isolated from the Korean red pepper genomic library for promoter analysis. The full-length 2.3-kb promoter and its deletion mutants similarly drove GUS expression in chromoplastic tissues such as anthers and styles in transgenic Arabidopsis flowers. However, the shortest 397-bp promoter (CaCcs-P1) containing the −367 to +30 region displayed histochemically high GUS expression in non-chromoplastic tissues such as sepals and leaves, as well as in chromoplastic tissues. The efficient constitutive expression of CaCcs-P1 was higher than that of the dual 35S promoter (d35S-P) with respect to mRNA expression (at least threefold) and enzyme activity (fourfold) and was maintained in the whole body of plants during all developmental stages. Ubiquitous GUS expression by CaCcs-P1 was elicited at the highest level in stems, followed by leaves, flowers, roots, young siliques, and mature seeds, with respect to both transcript levels and enzyme activity. More effective activity of CaCcs-P1 compared to d35S-P (1.4-fold higher) was also confirmed in transgenic tobacco plants. Together with the prediction of cis-acting motifs that allow diverse spatial specificity, we report a promoter displaying efficient constitutive characteristics with the advantages of a plant origin and size competitiveness compared with the 35S promoter.

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References

  • Bird CR, Smith CJS, Ray JA, Moureau P, Bevan MW, Bird AS, Hughes S, Morris PC, Grierson D, Schuch W (1988) The tomato polygalacturonase gene and ripening-specific expression in transgenic plants. Plant Mol Biol 11:651–662

    Article  CAS  PubMed  Google Scholar 

  • Bouvier F, Hugueney P, d’Harlingue A, Kuntz M, Camara B (1994) Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5, 6-epoxycarotenoid into ketocarotenoid. Plant J 6:45–54

    Article  CAS  PubMed  Google Scholar 

  • Bouvier F, Backhaus RA, Camara B (1998) Induction and control of chromoplast-specific carotenoid genes by oxidative stress. J Biol Chem 273:30651–30659

    Article  CAS  PubMed  Google Scholar 

  • Chung KJ, Hwang SK, Hahn BS, Kim KH, Kim JB, Kim YH, Yang JS, Ha SH (2008) Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis. Plant Cell Rep 27:29–37

    Article  CAS  PubMed  Google Scholar 

  • Dalal M, Chinnusamy V, Bansal KC (2010) Isolation and fuctional characterization of lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites. BMC Plant Biol 10:61

    Article  PubMed Central  PubMed  Google Scholar 

  • Deruère J, Bouvier F, Steppuhn J, Klein A, Camara B, Kuntz M (1994) Strructure and expression of two plant cells encoding chromoplast-specific proteins: occurrence of partially spliced transcripts. Biochem Biophys Res Commun 199:1144–1150

    Article  PubMed  Google Scholar 

  • Fang RX, Nagy F, Sivasubramaniam S, Chua NH (1989) Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. Plant Cell 1:141–150

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Grefen C, Donald N, Hashimoto K, Kudla J, Schumacher K, Blatt MR (2010) A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies. Plant J 64:355–365

    Article  CAS  PubMed  Google Scholar 

  • Ha SH, Lee SW, Kim JG, Hwang YS (1999) Expression patterns of genes involved in carotenoid biosynthesis in pepper. Agric Chem Biotechnol 42:92–96

    CAS  Google Scholar 

  • Ha SH, Kim JB, Park JS, Lee SW, Cho KJ (2007) A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper. J Exp Bot 58:3135–3144

    Article  CAS  PubMed  Google Scholar 

  • Ho MW, Ryan A, Cummins J (2000) Hazards of transgenic plants with the cauliflower mosaic viral promoter. Microb Ecol Health Dis 12:6–11

    Article  CAS  Google Scholar 

  • Kuntz M (2002) Gene promoter sequences and uses thereof. US Patent 6,437,221 B1, Aug 20, 2002

  • Lewin A, Jacob D, Freytag B, Appel B (1998) Gene expression in bacteria directed by plant-specific regulatory sequences. Transgenic Res 7:403–411

    Article  CAS  Google Scholar 

  • Liang YS, Bae H-J, Kang S-H, Lee T, Kim MG, Kim Y-M, Ha SH (2009) The Arabidopsis beta-carotene hydroxylase gene promoter for a strong constitutive expression of transgene. Plant Biotechnol Rep 3:325–331

    Article  Google Scholar 

  • Liang YS, Jeon YA, Lim SH, Kim JK, Lee JY, Kim YM, Lee YH, Ha SH (2011) Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter. Plant Cell Rep 30:973–980

    Article  CAS  PubMed  Google Scholar 

  • Lim SH, Ha SH (2013) Marker development for the identification of rice seed color. Plant Biotechnol Rep 7:391–398

    Article  Google Scholar 

  • Lim SH, Sohn SH, Kim DH, Kim JK, Lee JY, Kim YM, Ha SH (2012) Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant. Plant Biotechnol Rep 6:203–211

    Article  Google Scholar 

  • Lim SH, Kim DH, Kim JK, Lee JY, Kim YM, Sohn SH, Kim DH, Ha SH (2013) Petal-specific activity of the promoter of an anthocyanin synthase gene of tobacco (Nicotiana tabacum L.). Plant Cell Tiss Organ Cult 114:373–383

    Article  CAS  Google Scholar 

  • Malik K, Wu K, Li XQ, Martin-Heller T, Hu M, Foster E, Tian L, Wang C, Ward K, Jordan M, Brown D, Gleddie S, Simmonds D, Zheng S, Simmonds J, Miki B (2002) A constitutive gene expression system derived from the tCUP cryptic promoter elements. Theor Appl Genet 105:505–514

    Article  CAS  PubMed  Google Scholar 

  • Odell JT, Nagy F, Chua NH (1985) Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313:810–812

    Article  CAS  PubMed  Google Scholar 

  • Omirulleh S, Ábrahám M, Golovkin M, Stefanov I, Karabev MK, Mustárdy L, Mórocz S, Dudits D (1993) Activity of a chimeric promoter with the doubled CaMV 35S enhancer element in protoplast-derived cells and transgenic plants in maize. Plant Mol Biol 21:415–428

  • Park SH, Jeong JS, Han EH, Redillas MCFR, Bang SW, Jung H, Kim YS, Kim JK (2013) Characterization of the root-predominant gene promoter HPX1 in transgenic rice plants. Plant Biotechnol Rep 7:339–344

    Article  Google Scholar 

  • Podevin N, du Jardin P (2012) Possible consequences of the overlap between the CaMV 35S promoter regions in plant transformation vectors used and the viral gene VI in transgenic plants. GM Crops Food 3:296–300

    Article  PubMed  Google Scholar 

  • Ramchiary N, Kehie M, Brahma V, Kumaria S, Tandon P (2014) Application of genetics and genomics towards Capsicum translational research. Plant Biotechnol Rep 8:101–123

    Article  Google Scholar 

  • Xiao K, Zhang C, Harrison M, Wang ZY (2005) Isolation and characterization of a novel plant promoter that directs strong constitutive expression of transgene in plants. Mol Breed 15:221–231

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Grants from the Next-Generation BioGreen 21 Program (PJ011286012015 and PJ011094012015), Rural Development Administration, Republic of Korea, and by the Basic Science Research Program through the National Research Foundation of Korea (NRF-2013R1A1A2062998).

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Correspondence to Sun-Hwa Ha.

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Ha, SH. Competitive activity as a constitutive promoter in the 5′-proximal regulatory region of the Capsicum capsanthin–capsorubin synthase gene. Plant Biotechnol Rep 9, 259–267 (2015). https://doi.org/10.1007/s11816-015-0362-7

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