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Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

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Abstract

The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Korea

    Irisappan Ganesh & Soon Ho Hong

  2. Department of Pharmaceutical Science and Technology, Catholic University of Daegu, 330, Geumrak 1-ri, Hayang-eup, Gyeongsan-si, Gyeongbuk, 712-702, Korea

    Sambandam Ravikumar

  3. Department of Environmental Engineering and Energy, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggido, 449-728, Korea

    Si Jae Park

  4. Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon, 305-600, Korea

    Seung Hwan Lee

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  1. Irisappan Ganesh
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  2. Sambandam Ravikumar
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  3. Si Jae Park
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  4. Seung Hwan Lee
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  5. Soon Ho Hong
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Correspondence to Soon Ho Hong.

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Ganesh, I., Ravikumar, S., Park, S.J. et al. Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli . Korean J. Chem. Eng. 30, 1443–1447 (2013). https://doi.org/10.1007/s11814-013-0061-4

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  • DOI: https://doi.org/10.1007/s11814-013-0061-4

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