Abstract
This study compared the growth and lipid accumulation properties of two oleaginous microalgae, namely, Scenedesmus sp. LX1 and Chlorella sp. HQ, under different nutrient conditions. Both algal species obtained the highest biomass, lipid content and lipid yield under low-nutrient conditions (mBG11 medium). The biomass, lipid content and lipid yield of Scenedesmus sp. LX1 were 0.42 g·L−1, 22.5% and 93.8 mg·L−1, respectively. These values were relatively higher than those of Chlorella sp. HQ (0.30 g·L−1, 17.1% and 51.3 mg·L−1, respectively). These algae were then cultivated in an SE medium that contained more nutrients; as a result, the biomass and lipid yield of Scenedesmus sp. LX1 reduced more significantly than those of Chlorella sp. HQ. Opposite results were observed in lipid and triacylglycerols (TAGs) contents. The cell sizes of both algal species under low-nutrient conditions were larger than those under high-nutrient conditions. Chlorella sp. HQ cells did not aggregate, but Scenedesmus sp. LX1 cells flocculated easily, particularly under low-nutrient conditions. In summary, low-nutrient conditions favour the growth and lipid production of both algae, but Scenedesmus sp. LX1 outperforms Chlorella sp. HQ.
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References
Chisti Y. Biodiesel from microalgae. Biotechnology Advances, 2007, 25(3): 294–306
Deng X D, Li Y J, Fei X W. Microalgae: a promising feedstock for biodiesel. African Journal of Microbiology Research, 2009, 3(13): 1008–1014
Schenk P M, Thomas-Hall S R, Stephens E, Marx U C, Mussgnug J H, Posten C, Kruse O, Hankamer B. Second generation biofuels: high-efficiency microalgae for biodiesel production. BioEnergy Research, 2008, 1(1): 20–43
Mata T M, Martins A A, Caetano N S. Microalgae for biodiesel production and other applications: a review. Renewable & Sustainable Energy Reviews, 2010, 14(1): 217–232
Griffiths M J, Harrison S T L. Lipid productivity as a key characteristic for choosing algal species for biodiesel production. Journal of Applied Phycology, 2009, 21(5): 493–507
Gouveia L, Oliveira A C. Microalgae as a raw material for biofuels production. Journal of Industrial Microbiology & Biotechnology, 2009, 36(2): 269–274
Pittman J K, Dean A P, Osundeko O. The potential of sustainable algal biofuel production using wastewater resources. Bioresource Technology, 2011, 102(1): 17–25
Li X, Hu H Y, Ke G, Sun Y X. Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresource Technology, 2010, 101(14): 5494–5500
Converti A, Casazza A A, Ortiz E Y, Perego P, Del Borghi M. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification, 2009, 48(6): 1146–1151
Solovchenko A E, Khozin-Goldberg I, Didi-Cohen S, Didi-Cohen S, Cohen Z, Merzlyak M N. Effects of light and nitrogen starvation on the content and composition of carotenoids of the green microalga Parietochloris incisa. Russian Journal of Plant Physiology: a Comprehensive Russian Journal on Modern Phytophysiology, 2008, 55(4): 455–462
Li X, Hu H Y, Gan K, Yang J. Growth and nutrient removal properties of a freshwater microalga Scenedesmus sp. LX1 under different kinds of nitrogen sources. Ecological Engineering, 2010b, 36(4): 379–381
Zhang Q, Hong Y. Effects of stationary phase elongation and initial nitrogen and phosphorus concentrations on the growth and lipidproducing potential of Chlorella sp. HQ. Journal of Applied Phycology, 2013, doi: 10.1007/s10811-013-0091-7
State Environmental Protection Administration. Monitoring Method of Water and Wastewater, 4th ed. Beijing: China Environmental Science Press; 2002, 105, 246–248, 255–257
Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 1959, 37(8): 911–917
Dou X, Lu X H, Lu M Z, Yu L S, Xue R, Ji J B. The effects of trace elements on the lipid productivity and fatty acid composition of Nannochloropis oculata. Journal of Renewable Energy, 2013, 671545: 1–6.
Zhou X P, Xia L, Ge H M, Zhang D L, Hu C X. Feasibility of biodiesel production by microalgae Chlorella sp. (FACHB-1748) under outdoor conditions. Bioresource Technology, 2013, 138: 131–135
Liu Z Y, Wang G C, Zhou B C. Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresource Technology, 2008, 99(11): 4717–4722
Yang K. Selection of high-level lipid microalgae and effect of different culture conditions on the fatty acid contents and components. Dissertation for the Master’s Degree. Suzhou: Su Zhou University, 2009 (in Chinese)
Li X. Coupled technology of advanced N, P removal in wastewater treatment and microalgal bioenergy production. Dissertation for the Doctoral Degree. Beijing: Tsinghua University, 2011 (in Chinese)
Wang B, Li Y, Wu N, Lan C Q. CO2 bio-mitigation using microalgae. Applied Microbiology and Biotechnology, 2008, 79(5): 707–718
Griffiths E W. Removal and utilization of wastewater nutrients for algae biomass. Dissertation for the Doctoral Degree. Utah: Utah State University, 2009
Yu Y, Hu H Y, Li X, Wu Y H, Zhang X, Jia S L. Accumulation characteristics of soluble algal products (SAP) by a freshwater microalga Scenedesmus sp. LX1 during batch cultivation for biofuel production. Bioresource Technology, 2012, 110: 184–189
Su Y Y, Mennerich A, Urban B. Comparison of nutrient removal capacity and biomass settleability of four high-potential microalgal species. Bioresource Technology, 2012, 124: 157–162
Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici M R. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnology and Bioengineering, 2009, 102(1): 100–112
Cakmak T, Angun P, Demiray Y E, Ozkan A D, Elibol Z, Tekinay T. Differential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtii. Biotechnology and Bioengineering, 2012, 109(8): 1947–1957
Siaut M, Cuiné S, Cagnon C, Fessler B, Nguyen M, Carrier P, Beyly A, Beisson F, Triantaphylidès C, Li-Beisson Y H, Peltier G. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnology, 2011, 11(1): 7–22
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant Journal, 2008, 54(4): 621–639
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Zhang, Q., Hong, Y. Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions. Front. Environ. Sci. Eng. 8, 703–709 (2014). https://doi.org/10.1007/s11783-014-0649-x
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DOI: https://doi.org/10.1007/s11783-014-0649-x