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A comparative cell wall proteomic analysis of cucumber leaves under Sphaerotheca fuliginea stress

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Abstract

Powdery mildew, caused by Sphaerotheca fuliginea (S. fuliginea), is the most devastating disease that hampers cucumber plants cultivation and productivity. Cell wall proteins (CWPs) play a crucial role in response to biotic stress as a frontline defense of plants. In this work, we present a comparative cell wall proteomic approach to explore differentially expressed proteins in both highly resistant and highly susceptible cucumber leaves after 24 h of exposure to S. fuliginea. After extraction conducted by a destructive procedure with salts, glucose-6-phosphate dehydrogenase (G6PDH) activity and SDS-PAGE assessments were performed to determine the cytosolic contamination. Label-free quantitative proteomics approach was used to gain a comprehensive understanding of differentially regulated CWPs between the two lines after S. fuliginea inoculation. Among more than 200 proteins identified, 71 were significantly altered between the two lines. Most of these identified proteins were predicted to be CWPs except some classical cytosolic proteins. These differentially expressed CWPs belonged to different functional categories including defense, metabolism, redox regulation and cell wall arrangement. The expression levels of seven proteins selected were determined using RT-PCR. We found that resistant cucumber line is believed to start a series of disease-resistant mechanisms against pathogen. This study provides useful information on cell wall proteomic changes between a resistant and a susceptible genotype under infected conditions.

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Acknowledgments

This study was funded by the Program for Excellent Talents in University of Liaoning Province (LR2014019) and the Talents Engineering of Liaoning Province (2014921040). The authors thank Shanghai Hoogen Biotechnology Co., Ltd. for the technology support.

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    Authors and Affiliations

    1. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

      Xiangnan Meng, Haiyan Fan, Yang Yu & Na Cui

    2. Liaoning Academy of Agricultural Sciences, Shenyang, 110866, People’s Republic of China

      Tiefeng Song & Juyong Zhao

    3. Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

      Haiyan Fan

    4. Foreign Languages Department, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

      Kexin Meng

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    1. Xiangnan Meng
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    Communicated by M. Stobiecki.

    X. Meng and T. Song contributed equally to this work.

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    11738_2016_2266_MOESM1_ESM.xls

    Supplementary material 1 (XLS 90 kb) Supplemental Information Additional Supporting Information may be found in the online version of this article: Table S1 Proteins were identified in B21-a-2-1-2 and B21-a-2-2-2 based on intensity-based absolute quantification method (iBAQ)

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    Meng, X., Song, T., Fan, H. et al. A comparative cell wall proteomic analysis of cucumber leaves under Sphaerotheca fuliginea stress. Acta Physiol Plant 38, 260 (2016). https://doi.org/10.1007/s11738-016-2266-8

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