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Comparative response of two wucai (Brassica campestris L.) genotypes to heat stress on antioxidative system and cell ultrastructure in root

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Abstract

The effects on root growth, root antioxidant capacity, and cellular ultrastructure were investigated using two wucai genotypes (heat-tolerant WS-1 and heat-sensitive WS-6) under heat stress (40/30 °C) for 5 days. Heat stress caused decreases in root biomass, relative water content (RWC), root vigor, and root traits of two wucai genotypes. In addition, it resulted in reactive oxygen species (ROS) accumulation and increased hydrogen peroxide (H2O2) content, superoxide anion (O2 −) formation rate, and malondialdehyde (MDA) content, but the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were inhibited to different extents in two genotype wucai roots. However, these data indicated that the decline extent of WS-1 (heat tolerant) in root growth and antioxidant capacity was significantly lower than that of WS-6 (Heat sensitive). Microscopic analyses revealed that WS-1 (heat tolerant) showed a better cellular shape than WS-6 under heat stress and slightly oxidative damage; nuclear and mitochondria in WS-1 were of a better intact shape and clear bilayer membrane. Most importantly, the thicker root cell wall in heat-tolerant wucai genotype responding to heat stress was first reported. These results suggested that the ability of heat-tolerant wucai genotype to minimize the heat stress depended upon the higher self-regulation capacity and effectiveness of the antioxidant metabolism.

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Abbreviations

CAT:

Catalase

DW:

Dry weights

FW:

Fresh weights

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

O2 − :

Superoxide anion

POD:

Peroxidase

ROS:

Reactive oxygen species

RWC:

Relative water content

SOD:

Superoxide dismutase

TW:

Turgor weights

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Acknowledgments

This work was supported by the Agriculture Science Technology Achievement Transformation Fund of China (2014GB2C300018), the Provincial Natural Science Foundation of Anhui, China (1608085QC48), and Anhui College Youth Fund Project (2015ZD18).

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    Authors and Affiliations

    1. Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China

      Lingyun Yuan, Shan Liu, Shidong Zhu, Guohu Chen, Fan Liu, Mingqian Zou & Chenggang Wang

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    1. Lingyun Yuan
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    2. Shan Liu
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    3. Shidong Zhu
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    4. Guohu Chen
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    5. Fan Liu
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    Correspondence to Chenggang Wang.

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    Communicated by W. Wang.

    L. Yuan and S. Liu contributed equally to this paper.

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    Yuan, L., Liu, S., Zhu, S. et al. Comparative response of two wucai (Brassica campestris L.) genotypes to heat stress on antioxidative system and cell ultrastructure in root. Acta Physiol Plant 38, 223 (2016). https://doi.org/10.1007/s11738-016-2246-z

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    • DOI: https://doi.org/10.1007/s11738-016-2246-z

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