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Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

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Abstract

The present work deals with optimization of adventitious shoot culture of Bacopa monnieri for the production of biomass and bacoside A and has investigated the effects of macro elements (NH4NO3, KNO3, CaCl2, MgSO4 and KH2PO4) and nitrogen source [NH4 +/NO3 −] of Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) on accumulation of biomass and bacoside A content. Optimum number of adventitious shoots (99.33 shoots explant−1), fresh weight (1.841 g) and dry weight (0.150 g) were obtained in the medium with 2.0× strength of NH4NO3. The highest production of bacoside A content was also recorded in the medium of 2.0× NH4NO3, which produced 17.935 mg g−1 DW. The number of adventitious shoot biomass and bacoside A content were optimum when the NO3 − concentration was higher than that of NH4 +. Maximum number of shoots (70.00 shoots explant−1), biomass (fresh weight 1.137 g and dry weight 0.080 g) and also bacoside A content (27.106 mg g−1 DW) were obtained at NH4 +/NO3 − ratio of 14.38/37.60 mM. Overall, MS medium supplemented with 2.0× NH4NO3 is recommended for most efficient bacoside A production.

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Fig. 1

Abbreviations

ANOVA:

Analysis of variance

HPLC:

High performance liquid chromatography

Kin:

Kinetin

MS:

Murashige and Skoog (1962) medium

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Acknowledgments

This work was partially financed by Council of Scientific and Industrial Research [No. 38(1098)/04/EMR-II] and University Grants Commission under the Special Assistance Programme, New Delhi, India.

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Authors and Affiliations

  1. Department of Botany, Karnatak University, Dharwad, 580 003, India

    Poornananda Madhava Naik, Shirugumbi Hanamanthagouda Manohar & Hosakatte Niranjana Murthy

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  1. Poornananda Madhava Naik
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  2. Shirugumbi Hanamanthagouda Manohar
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  3. Hosakatte Niranjana Murthy
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Correspondence to Hosakatte Niranjana Murthy.

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Communicated by S. Lewak.

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Naik, P.M., Manohar, S.H. & Murthy, H.N. Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.). Acta Physiol Plant 33, 1553–1557 (2011). https://doi.org/10.1007/s11738-010-0675-7

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