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A micropropagation protocol for Cassia angustifolia Vahl. from root explants

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Abstract

The aim of this study was to develop a new micropropagation system for Cassia angustifolia Vahl., an important medicinal legume using root explant as starting material. Root explants taken from 30-day-old aseptic seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators: 6-benzyladenine (BA), kinetin (Kn), and thidiazuron (TDZ). Organogenic nodular calli obtained on MS + TDZ (1.0 μM) were transferred to shoot regeneration medium supplemented with different cytokinins (BA, Kn or TDZ) either alone or in combination with auxin:indole-3-acetic acid or α-naphthalene acetic acid. Maximum shoot regeneration frequency (90%) was obtained on MS + BA (2.5 μM) + NAA (0.6 μM) wherein a maximum of 42.76 ± 1.47 shoot buds per explant were induced with a maximum conversion rate of 35.63 ± 0.75 shoots per explant and average shoot length of 5.43 ± 0.20 cm. Elongated microshoots were successfully rooted under ex vitro conditions by pulse treatment in 200 μM of indole-3-butyric acid for half an hour. Microshoots were rooted, acclimatized and hardened off simultaneously in sterilized soilrite inside the growth room and then established in pots containing sterilized soil and manure (1:1) and grown under greenhouse condition with 90% survival rate. The histological sections at different developmental stages of shoot buds revealed the organization of nodular meristematic zone leading to the orientation and differentiation of shoot buds in large number and thereafter conversion into healthy shoots.

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Acknowledgments

The author Anwar Shahzad greatly acknowledged the financial support promoted by the Department of Science and Technology (DST), Government of India, New Delhi in the form of SERC Fast Track Scheme Vide no. SR/FT/L-23/2006. The authors are also thankful to the Department of Science and Technology (DST) Government of India, New Delhi for providing research assistance under DST-FIST Programme 2005 (Project No. SR/FST/LSI-085/2005).

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  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202002, UP, India

    Shahina Parveen & Anwar Shahzad

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  1. Shahina Parveen
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  2. Anwar Shahzad
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Correspondence to Anwar Shahzad.

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Communicated by B. Borkowska.

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Parveen, S., Shahzad, A. A micropropagation protocol for Cassia angustifolia Vahl. from root explants. Acta Physiol Plant 33, 789–796 (2011). https://doi.org/10.1007/s11738-010-0603-x

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  • DOI: https://doi.org/10.1007/s11738-010-0603-x

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