Abstract
Two genotypes of rice (Oryza sativa L.), Azucena (iron tolerant) and IR64 (iron sensitive), were used to investigate the numbers and survival rates of root border cells (namely, in situ border cells) in plants that were exposed to excess iron (Fe2+). Additionally, we examined the changes in the root tip cell morphology and activities of protective enzymes in response to Fe2+ toxicity. The results showed that Fe2+ toxicity hindered the development of root border cells (RBCs) and that higher Fe2+ concentrations caused root cap cell walls to thicken. In the iron-sensitive rice variety, these changes lowered RBC survival rate and lead to programmed cell death. Low concentrations of Fe2+ were shown to facilitate the development of RBCs in the iron-tolerant rice variety and that the activities of the protective enzymes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were elevated in the iron-tolerant variety, thus suggesting that rice root tips could defend against Fe2+ toxicity by producing RBCs, root cap cells, and protective enzymes.
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Abbreviations
- RBC:
-
Root border cell
- POD:
-
Peroxidase
- CAT:
-
Catalase
- SOD:
-
Superoxide dismutase
- EM:
-
Electron microscopy
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Acknowledgments
The authors would like to thank the National Rice Medium-term Seed Storage Vault for providing rice seeds. This work was supported by a grant from the Natural Science Foundation of Zhejiang Province, China (Nos. Y305102, Y307408, Y5090339, Y5100390).
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Communicated by M. N. V. Prasad and A. K. Kononowicz.
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Zhang, Y., Zheng, G.H., Liu, P. et al. Morphological and physiological responses of root tip cells to Fe2+ toxicity in rice. Acta Physiol Plant 33, 683–689 (2011). https://doi.org/10.1007/s11738-010-0590-y
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DOI: https://doi.org/10.1007/s11738-010-0590-y